Progress of the Electrochemiluminescence Biosensing Strategy for Clinical Diagnosis with Luminol as the Sensing Probe

Chen, Fang; Li, Huiling; Yan, Jilin; Guo, Heming; Tu, Yifeng

doi:10.1002/celc.201700465

Cited by 54 publications

(32 citation statements)

References 121 publications

(156 reference statements)

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“…It can emit ECL under a wide range of experimental conditions and has the advantages of high quantum yield, good water solubility and low cost. 45 Luminol has been widely used in inorganic analysis, organic analysis and immunoassay. 46 More significantly, the use of luminol is sufficiently permissive without any limit of patent right as other luminescent probes, such as tris(2,2-bipyridyl) ruthenium.…”

Section: Resultsmentioning

confidence: 99%

A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody

et al. 2019

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An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.

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Section: Resultsmentioning

confidence: 99%

A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody

et al. 2019

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“…Luminol is often produced in alkaline solution via a co-reactant pathway with hydrogen peroxide or other reactive oxygen species (ROS) as the co-reactant [2,[49][50]. Emission of ECL results from the excited state in 3-aminophtalate, which has maximum wavelengths at 420 nm [34,48].…”

Section: Luminolmentioning

confidence: 99%

Electrogenerated Chemiluminescence for Immunoassay Applications

et al. 2021

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Electrogenerated chemiluminescence (ECL) has recently become one of the most prominent and well-established transducers for immunoassay techniques. ECL relates a luminophore concentration in solution with the emission of light triggered by an electrochemical stimulus. ECL immunoassay (ECLIA) performance depends on the parameters of its light generation, including the luminophore, the species that emit light called labels in ECLIA; co-reactants, which are added reagents that support the luminophore to undergo the excited state; electrodes, which are the place for the ECL reactions to take place; and the format of the immunoassay. This review discusses the behaviour of ECLIA parameters, the required instrumentations, and some important examples of detections based on ECLIA.

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“…Another factor to consider in the case of ECL biosensors is the luminophore, which can be trapped together with the enzymes on the electrode surface or found in solution. Three main luminophores are used in enzyme biosensor such as [Ru(bpy) 3 ] 2+ , luminol [ 97 ] and quantum dots (QDs) [ 98 ], being luminol the most used in oxidase enzyme biosensors. The light emission of luminol at the electrodes was first used in 1929 [ 99 ].…”

Section: Ecl Enzymatic Biosensorsmentioning

confidence: 99%

“…Because many oxide reductase enzymes can produce H 2 O 2 during their catalytic activity, ECL enzyme biosensors can be carried out by coupling the light-emitting reaction produced by luminol with enzyme-catalyzed reactions that generate H 2 O 2 . Hence, luminol and its derivatives have been coupled with oxidase enzymes for the generation of ECL [ 97 ].…”

Section: Ecl Enzymatic Biosensorsmentioning

confidence: 99%

Electrochemiluminescence Biosensors Using Screen-Printed Electrodes

et al. 2020

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Electrogenerated chemiluminescence (also called electrochemiluminescence (ECL)) has become a great focus of attention in different fields of analysis, mainly as a consequence of the potential remarkably high sensitivity and wide dynamic range. In the particular case of sensing applications, ECL biosensor unites the benefits of the high selectivity of biological recognition elements and the high sensitivity of ECL analysis methods. Hence, it is a powerful analytical device for sensitive detection of different analytes of interest in medical prognosis and diagnosis, food control and environment. These wide range of applications are increased by the introduction of screen-printed electrodes (SPEs). Disposable SPE-based biosensors cover the need to perform in-situ measurements with portable devices quickly and accurately. In this review, we sum up the latest biosensing applications and current progress on ECL bioanalysis combined with disposable SPEs in the field of bio affinity ECL sensors including immunosensors, DNA analysis and catalytic ECL sensors. Furthermore, the integration of nanomaterials with particular physical and chemical properties in the ECL biosensing systems has improved tremendously their sensitivity and overall performance, being one of the most appropriates research fields for the development of highly sensitive ECL biosensor devices.

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Progress of the Electrochemiluminescence Biosensing Strategy for Clinical Diagnosis with Luminol as the Sensing Probe

Cited by 54 publications

References 121 publications

A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody

A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody

Electrogenerated Chemiluminescence for Immunoassay Applications

Electrochemiluminescence Biosensors Using Screen-Printed Electrodes

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