Electrochemiluminescence Biosensors Using Screen-Printed Electrodes

Abstract

Electrogenerated chemiluminescence (also called electrochemiluminescence (ECL)) has become a great focus of attention in different fields of analysis, mainly as a consequence of the potential remarkably high sensitivity and wide dynamic range. In the particular case of sensing applications, ECL biosensor unites the benefits of the high selectivity of biological recognition elements and the high sensitivity of ECL analysis methods. Hence, it is a powerful analytical device for sensitive detection of different analytes of interest in medical prognosis and diagnosis, food control and environment. These wide range of applications are increased by the introduction of screen-printed electrodes (SPEs). Disposable SPE-based biosensors cover the need to perform in-situ measurements with portable devices quickly and accurately. In this review, we sum up the latest biosensing applications and current progress on ECL bioanalysis combined with disposable SPEs in the field of bio affinity ECL sensors including immunosensors, DNA analysis and catalytic ECL sensors. Furthermore, the integration of nanomaterials with particular physical and chemical properties in the ECL biosensing systems has improved tremendously their sensitivity and overall performance, being one of the most appropriates research fields for the development of highly sensitive ECL biosensor devices.

Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

“…Compared with the traditional methods used to determine certain compounds, the great versatility of SPEs is due to the wide range of strategies in which the working electrode surface can be modified [27]. In addition to the possibility of using a wide variety of compounds to change the composition in order to control the electrochemical behavior, different strategies for modifying the surface of SPEs have allowed the employment of these electrodes in a variety of sensors and biosensors [28]. Another key factor of SPE modification was the appearance of a great variety of nanomaterials.…”

An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma

“…Electrochemiluminescence resonance energy transfer (ECL-RET) biosensor is a promising technology with high sensitivity and a large concentration response range for the detection and quantification of different analytes including nucleic acids, antigens, metabolites, and whole cells ( Chen et al., 2014 ; Chen et al., 2019 ; Chen et al., 2020a ; Tian et al., 2012 ; Wu et al., 2011 ; Zhang et al., 2008 ; Martínez-Periñán et al., 2020 ). In ECL-RET biosensors designed for DNA detection, semiconductor quantum dots (QDs) such as eco-friendly SiQDs or graphitic-carbon nitride QDs (g-CNQDs) coated on a glassy carbon electrode (GCE) generate an electrochemiluminescence (ECL) signal ( Scheme 1 ) ( Liu et al., 2019 ; Dong et al., 2017 ).…”

Carbon nanodot–based electrogenerated chemiluminescence biosensor for miRNA-21 detection

“…The energy from the excitation source in ECL assays has a different nature than the signal, compared to fluorescence, giving ECL certain advantages such as a high signal to noise ratio. It therefore has great potential as a detection system for (bio)sensors [14,15]. ECL-based biosensors have demonstrated remarkable properties and advantages such as controllability, high sensitivity, and low background noise.…”