Reactive oxygen species and cell morphology of MC3T3E1 pre-osteoblast cell line exposed to methylgyoxal by laser scanning confocal microscopy

Akbar, Izaak Zoelkarnain; Permatasari, Nur; Soeatmadji, Djoko Wahono; Hidayat, M. Taufik; Kalim, Handono

doi:10.5455/oams.050313.br.006

Cited by 4 publications

(3 citation statements)

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“…MC3T3E1 preosteoblast cell line subclone 4 was obtained from American Type Culture Cell Collection (ATCC). MC3T3E1 preosteoblast cell line was exposed to MG at concentration 5 µM for 6 hours of incubation time [12]. Oxidant pathway obtained using blockade of superoxide dismutase blockade (Diethylthiocarbamoic acid/DETCA), blockade of gluthatione peroxidase (mercaptosuccinate/ MS), and iron chelating (defferoxamine/DFX).…”

Section: Cell Linementioning

confidence: 99%

EFFECT OF METHYLGLYOXAL ON SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS (sRAGE) OF PRE-OSTEOBLAST MC3T3E1 CELL LINE

Akbar¹,

Permatasari²,

Soeatmadji³

et al. 2014

Gulhane Med J

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Methylgyoxal (MG) is reactive dicarbonyl compound was found in high level in blood of diabetic patients. Methlyglyoxal was more reactive compared to glucose and have toxic effect through AGEs. Soluble Receptor for Advanced Glycation End Products (sRAGE) act as a scavenger and decoy receptor for AGEs. This study aimed to investigate the effect of changes intracellularly redox status on sRAGE levels in preosteoblast MC3T3E1 exposed to MG. The changes of redox status was obtained by blockade of superoxide dismutase (Diethylthiocarbamoic acid/DETCA), blockade of gluthatione peroxidase (mercapto succinate/MS), and chelating of iron (defferoxamine/DFX). sRAGE levels was evaluated by enzyme linked imunnosorbent assay technique. The levels of sRAGE in MG-exposed group or DETCA-exposed group was significantly increased than that in control group (P < 0.05). The levels of sRAGE in MG+DETCA-exposed group was significantly decrease compared with DETCA-exposed group (P < 0.05). The levels of sRAGE was significantly increased in MS-exposed group or MG+MS-exposed group than that in control group, respectively (P < 0.05). Concluded that the change of redox status by antioxidant blocker or MG exposure would increases sRAGE levels in preosteoblast MC3T3E1 cell line. The modulation of redox state by antioxidant blocker does not affect sRAGE level in the presence of MG. Iron chelating does not change the sRAGE level.

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Section: Cell Linementioning

confidence: 99%

EFFECT OF METHYLGLYOXAL ON SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS (sRAGE) OF PRE-OSTEOBLAST MC3T3E1 CELL LINE

Akbar¹,

Permatasari²,

Soeatmadji³

et al. 2014

Gulhane Med J

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“…AGEs consist of several very heterogeneous components, where their number will increase in patients with diabetes, kidney failure, Alzheimer's disease, and in the aging process. The formation of AGE consists of several processes; Maillard reactions are characterized by the formation of a brown substance produced from the non-enzymatic reaction of the reduction of sugars and amino acids on proteins [1], [2]. This reaction evolved from the bond between the amino group of proteins with the carbonyl group of sugars to form the Schiff bases and Amadori components, thus leading to the formation of various AGE derivatives [3], [4].…”

Section: Introductionmentioning

confidence: 99%

Virtual Screening of the Active Components of Garcinia mangostana Linn. Potentially Inhibiting the Interaction of Advanced Glycation End-products and their Receptor

Faisal

Oktaviyanti

Sujuti

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Mangosteen (Garcinia mangostana L.) is a plant that contains various secondary metabolite compounds, one of which is xanthone. Xanthone in mangosteen has a variety of beneficial biological and medical effects, one of which is an antioxidative, anti-inflammatory, and antiapoptotic agent. AIM: The aim of the study was to perform the selection of any xanthone in mangosteen pericarp that have potentially inhibit the interaction of AGEs and RAGE. METHODS: The analysis was made in silico by docking method using software Hex 8.0. The docking was done between AGEs-RAGE, also between nine active compounds of G. mangostana with RAGE. The active compounds analyzed here were including α-mangostin, β-mangostin, γ-mangostin, mangostanol, garcinone D, 1,6-Dihydroxy-3,7-dimethoxy-2-(3-methylbut-2-enyl)-xanthone, gartanin, 1-isomangostin, and 3-isomangostin. Further analysis was performed to see the interactions formed between ligands with their receptors using software LigPlus+ and Discovery Studio 4.1. RESULTS: 1-isomangostin, 3-isomangostin, γ-mangostin, mangostanol, D-garcinone, and gartanin have potentially could inhibit the interaction and activity of imidazole in RAGE through a competitive binding mechanism. CONCLUSIONS: The inhibition of imidazole-RAGE activity by the mangosteen active components may inhibit the pathobiology of AGEs-RAGE axis.

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“…Non-enzymatic glycation product and advanced glycation end products (AGEs) acquire gradually in a wide diversity of environments. Maillard reaction firstly characterized by the formation of brown-colored substances resulted from nonenzymatic reaction come off between reducing sugars and amino acids of protein ( 5 , 6 ). This reaction is develop from chemical linkage between the amino group of protein and the carbonyl groups of sugar to form Schiff bases and Amadori compounds, resulting in heterogeneous derivatives termed AGEs ( 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

Fatchiyah¹,

Hardiyanti²,

Widodo³

2015

Acta Inform Med

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Background:Advanced Glycation End Products (AGE) play a pivotal role in the development various degenerative diseases such as diabetes, cardiovascular disease, stroke, neuropathy, and nephropathy. Different studies have been done to employ AGEs as drug targets for the diseases therapy. In previous study, we have found bioactive peptide from Ethawah goat milk for anti-diabetic that may work through inhibition of AGE receptor function. However, the mechanism of bioactive peptides inhibits AGE- AGE receptor (RAGE) bonding still not clear yet. Therefore we investigated the inhibition mechanism by calculate the potential energy binding among the peptides, AGEs and RAGE using molecular docking system.Methods:Modeling 3D-structure was predicted by SWISS-MODEL web server. The virtual interaction was analyzed by docking system using HEX 8.0, Pymol and Discovery Studio 4.0 software.Results:this study showed that AGEs (Argypirimidine, Imidazole, Pentosidine and Pyrraline) bind to C-domain of RAGE. The total energy binding of RAGE with Argypirimidine, Imidazole, Pentosidine and Pyrraline were 378.35kJ/mol, -74.57kJ/mol, -301.25kJ/mol and -400.72kJ/mol, respectively. We have found three peptides among eight peptides from Ethawah goat milk, which are able bind to C-domain of RAGE, there are CSN1S2 f41-47, CSN1S2 f182-189, and CSN1S2 f214-221. The CSN1S22 f41-47 at arginine residue 47 interacts with proline162, leusine163 and leusine158 of RAGE. The total binding energy between CSN1S2 f41-47, CSN1S2 f182-189, and CSN1S2 f214-221 with RAGE were -378.35 kJ/mol, -359.97kJ/mol, -356.78 kJ/mol, respectively. Total binding energy and binding pattern indicated that RAGE more prefer bind with peptide and block AGE bind to functional site of RAGE. Further analysis showed that complex peptide-RAGE shifted binding site of AGE on function domain RAGE.Conclusion:This study suggested that the peptides from Ethawah goat milk may act as an inhibitor of AGEs-RAGE interaction that impaired signal transduction cascade at the cellular level.

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Reactive oxygen species and cell morphology of MC3T3E1 pre-osteoblast cell line exposed to methylgyoxal by laser scanning confocal microscopy

Cited by 4 publications

References 0 publications

EFFECT OF METHYLGLYOXAL ON SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS (sRAGE) OF PRE-OSTEOBLAST MC3T3E1 CELL LINE

EFFECT OF METHYLGLYOXAL ON SOLUBLE RECEPTOR FOR ADVANCED GLYCATION END PRODUCTS (sRAGE) OF PRE-OSTEOBLAST MC3T3E1 CELL LINE

Virtual Screening of the Active Components of Garcinia mangostana Linn. Potentially Inhibiting the Interaction of Advanced Glycation End-products and their Receptor

Selective Inhibition on RAGE-binding AGEs Required by Bioactive Peptide Alpha-S2 Case in Protein from Goat Ethawah Breed Milk: Study of Biological Modeling

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