Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE

Fabric phase sorptive extraction for the fast isolation of sulfonamides residues from raw milk followed by high performance liquid chromatography with ultraviolet detection

“…For the isolation of sulfonamides in milk, various sample pretreatment techniques have been proposed including solid phase extraction (SPE) (Hou et al, 2014;Koesukwiwat, Jayanta, & Leepipatpiboon, 2007a, 2007bSamanidou, Tolika, & Papadoyannis, 2008), stir bar sorptive extraction (SBSE) based on polymer monolith material (Huang, Qiu, & Yuan, 2009), molecularly imprinted polymer (RA-MIP) with restricted access and selectivity for sulfonamides (Xu et al, 2010), a miniaturized graphene-based pipette tip extraction (M-G-PTE) (Yan, Sun, Liu, Ho, & Song, 2014) and liquid-liquid extraction (Nebot, Regal, Miranda, Fente, & Cepeda, 2013;Tolika et al, 2011).…”

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

“…Determination of SAs was initially performed by thin-layer chromatography [12], but at present, separation and quantification of sulfonamide residues are commonly achieved by high-performance liquid chromatography [13][14][15][16][17][18][19][20][21][22][23]. For analysis of feed samples, which compared with animal tissues are a quite complex matrix, use of mass spectrometry allows multiresidue detection of SAs, ensuring accurate and sensitive determinations [24]. Nevertheless, for official control analysis of feed samples, LC coupled with UV detection represents a valid alternative, providing good results in terms of selectivity, sensitivity, instrument cost, and simplicity.…”

Food Sample Preparation for the Determination of Sulfonamides by High-Performance Liquid Chromatography: State-of-the-Art

“…SPE [55], MSPE [57], and ATPS extraction [53,54] were the most reported approaches for the extraction of SAs from milk samples. Other approaches include LLE [49], DLLME [50], as well as a modified QuEChERS extraction protocol that employed C18, MgSO 4 and PSA [50], SALLE combined with SPE [51], and a miniaturized SALLE protocol [52]. Interesting approaches include FPSE [60], GMeS microextraction [61], and mini-SAE [62].…”

“…The combined organic phases were evaporated to dryness under nitrogen stream at 40 • C and the residue was dissolved in acidified MeOH (100 µL) and filtered prior to HPLC-MS/MS analysis. The authors emphasized the reduced sample and reagent requirements of the developed sample preparation protocol, while a SPE clean-up step was omitted in order to simplify and reduce the cost of the protocol [49]. DLLME and modified QuEChERS extraction, were reported for the extraction of nine SAs from milk samples.…”