Food Sample Preparation for the Determination of Sulfonamides by High-Performance Liquid Chromatography: State-of-the-Art

Bitas, Dimitrios; Kabir, Abuzar; Locatelli, Marcello; Samanidou, Victoria

doi:10.3390/separations5020031

Cited by 40 publications

(22 citation statements)

References 60 publications

(130 reference statements)

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“…However, the improper administration of sulfa drugs in dairy husbandry and the insufficient withdrawal periods can lead to noncompliant residues in animal originated foods, a fact which can contribute to several concerns in the dairy industry and public health [4].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides

2019

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In the present study, a novel, simple, and fast sample preparation technique is described for the determination of four sulfonamides (SAs), namely Sulfathiazole (STZ), sulfamethizole (SMT), sulfadiazine (SDZ), and sulfanilamide (SN) in cow milk prior to HPLC. This method takes advantage of a novel material that combines the extractive properties of graphene oxide (GO) and the known properties of common polyurethane sponge (PU) and that makes sample preparation easy, fast, cheap and efficient. The PU-GO sponge was prepared by an easy and fast procedure and was characterized with FTIR spectroscopy. After the preparation of the sorbent material, a specific extraction protocol was optimized and combined with HPLC-UV determination could be applied for the sensitive analysis of trace SAs in milk. The proposed method showed good linearity while the coefficients of determination (R2) were found to be high (0.991–0.998). Accuracy observed was within the range 90.2–112.1% and precision was less than 12.5%. Limit of quantification for all analytes in milk was 50 μg kg−1. Furthermore, the PU-GO sponge as sorbent material offered a very clean extract, since no matrix effect was observed.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides

2019

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“…Despite the extensive use of antibiotics in animals, reliable data about the pattern of use and the quantity of these compounds are not always available [ 22 ]. That is why it is important to monitor the concentration levels of antibiotic residues in animals and in food products of animal origin [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. Studies on the quantitative risk assessment of foods provide important frameworks to address some of the issues related to the risk to human health from the intake of various residues via food [ 32 , 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary Exposure Assessment of Veterinary Antibiotics in Pork Meat on Children and Adolescents in Cyprus

Kyriakides

Lazaris

Arsenoglou

et al. 2020

Foods

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In recent years, huge amounts of antibiotics have been administered to farm animals, and as a result, residues of these antibiotics can accumulate in livestock products and, once consumed, may be transmitted to humans. Farm animals’ antibiotic treatment may therefore present a risk for consumers health, especially for children and adolescents. In children, the immune system is not fully developed, and thus, they are more susceptible than adults to resistant bacteria. A dietary exposure assessment was conducted on veterinary antibiotics found in raw pork meat among children and adolescents in Cyprus, since pork is the most consumed red meat in Cypriot population. The study was based on the results of the occurrence of 45 residual antibiotics in raw pork meat samples in Cyprus between 2012 and 2017 in combination with data on the consumption of pork meat on children and adolescents taken from the latest demographic report in Cyprus. Estimated daily intake (EDI) values of veterinary antibiotics for children aged 6–9 years old, were higher compared to EDI values for adolescents aged 10–17 years old. The percentage ratio of the estimated daily intake to the acceptable daily intake for all the veterinary antibiotic residues was less than 5.6. The results indicate that antibiotic residues in pork meat of inland production are below the acceptable daily intake and are of low risk to human health related to the exposure of antibiotics. Nevertheless, continuous exposure to low levels of antibiotic residues in respect to age vulnerability should be of a great concern.

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“…To overcome these problems, chromatographic techniques, such as high-performance liquid chromatography (HPLC), was developed and known to be the most reliable, sensitive and robust method [21–23]. Available litreture showed that, HPLC was able to determine sulfonamides from food samples [24] and also HPLC method was successfully used to detect the antibiotic residues at lower than their respective maximum residue limits values [25]. Thus, in the present study the microbiological assay was first carried out to detect the presence of antibiotic residues in the cow milk samples collected from different farms in Dharwad district of Karnataka, India.…”

Section: Introductionmentioning

confidence: 99%

Detection and determination of stability of the antibiotic residues in cow’s milk

et al. 2019

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In the present study, antibiotic residues were detected in milk samples collected from the dairy herds located in Karnataka, India, by microbiological assay. Subsequently, the detected antibiotics were identified as azithromycin and tetracycline, by high-performance liquid chromatography, further both the antibiotics detected in the cow milk samples were found to be at high concentration (9708.7 and 5460 μg kg-1, respectively). We then investigated the effects of temperature and pH on the stabilities of azithromycin and tetracycline to determine the degradation rate constant k using first-order kinetic equation. Results indicated that significant reduction in stability and antibacterial activity of azithromycin solution when subjected to 70 and 100°C for 24 h. While stability of tetracycline was significantly reduced when subjected to 70 and 100°C for 24 h. However no significant reduction in antibacterial activity of tetracycline was observed at respective temperatures when compared with that of control. In addition, the stabilities of azithromycin and tetracycline were found to be decreased in acidic pH 4–5. The results of the present study revealed the high risk of contamination of milk sample with veterinary antibiotics and also demonstrated the effect of temperature and pH on stability of antibiotics. Therefore the study suggest that the qualitative and quantitative screening of milk for the presence of antibiotics need to be strictly performed to ensure safe drinking milk for consumers.

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Food Sample Preparation for the Determination of Sulfonamides by High-Performance Liquid Chromatography: State-of-the-Art

Cited by 40 publications

References 60 publications

Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides

Synthesis of Graphene Oxide Based Sponges and Their Study as Sorbents for Sample Preparation of Cow Milk Prior to HPLC Determination of Sulfonamides

Dietary Exposure Assessment of Veterinary Antibiotics in Pork Meat on Children and Adolescents in Cyprus

Detection and determination of stability of the antibiotic residues in cow’s milk

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