Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability

Fonseca, Dania A.; Gutiérrez, Humberto; Collins, Kenneth E.; Collins, Carol H.

doi:10.1016/j.chroma.2003.12.033

Cited by 23 publications

(16 citation statements)

References 32 publications

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“…These more drastic conditions have permitted rapidly obtaining results of column lifetimes without loss of information, when compared to stability tests that use neutral mobile phases. 91 This method has made it possible to quantify the improved stability of metalized silica-based stationary phases in relation to bare silica-based phases having the same immobilized polymer. These authors showed that a zirconized silica-based PMOS phase was stable to the passing of 600 mL of mobile phase at pH 10, while the bare silica-based counterparts collapsed after 150 mL.…”

Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

“…In addition, residual zirconol (Zr-OH) and titanol (Ti-OH) groups may behave as Lewis acid sites. [81][82][83][84][85][86][87][88][89][90][91][92][93][94] Fonseca 95 observed that separation of a test mixture containing phenol, N,N-dimethylaniline, naphthalene and acenaphthene with a titanized-silica based PMOS phase showed inversion of the elution order of N,N-dimethylaniline and naphthalene peaks due to the strong interaction between the basic analyte and residual groups. This interaction was more pronounced with lower immobilization times or temperatures of the thermal treatment used to prepare the PMOS phases.…”

Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

“…Fonseca et al 91 developed an accelerated stability test, using higher temperatures and mobile phases containing inorganic salts to evaluate the chemical stability of the stationary phases prepared at LabCrom. These conditions were used to accelerate the degradation of the stationary phase, reducing the evaluation time and, principally, the consumption of the solvents used in the stability tests.…”

Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

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State-of-the-art in immobilized polymer stationary phases for high-performance liquid chromatography

Faria¹,

Jardim²

2009

J. Braz. Chem. Soc.

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O aumento da estabilidade química e a redução da alta atividade de grupos residuais têm sido, nas últimas quatro décadas, os principais focos de pesquisas na preparação de fases estacionárias para cromatografia líquida de alta eficiência na modalidade de fase reversa (CLAE-FR). Novas e eficientes estratégias de modificação da superfície da sílica e a introdução de novos materiais como suportes cromatográficos têm minimizado estas limitações e possibilitado uma crescente popularização da CLAE-FR. Neste trabalho será apresentada uma visão geral do estado da arte de uma das estratégias de preparo de fases estacionárias que vem contornando tais limitações da CLAE-FR; as fases estacionárias baseadas na imobilização de polímeros orgânicos pré-sintetizados sobre partículas de sílica e sílica metalizada (zirconizada e titanizada). Uma consideração especial será dada ao desenvolvimento destas fases estacionárias ocorrido no Brasil nos últimos 15 anos.Increasing stability and reducing the high activity of residual groups from stationary phases have been the main focuses of research on the preparation of reversed-phase high-performance liquid chromatography (RP-HPLC) phases over the last four decades. New and more efficient strategies for modifying the silica surface as well as the introduction of new chromatographic supports have minimized these effects and have enabled the increasing popularization of RP-HPLC. In this paper, an overview will be given of the state-of-the-art in stationary phases for RP-HPLC based on immobilization of pre-synthesized organic polymers on both silica and metalized-silica supports. Special consideration is given to the development of HPLC stationary phases in Brazil in the past 15 years.

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Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

Section: Metalized Silica-based Immobilized Polymer Stationary Phasesmentioning

confidence: 99%

See 1 more Smart Citation

State-of-the-art in immobilized polymer stationary phases for high-performance liquid chromatography

Faria¹,

Jardim²

2009

J. Braz. Chem. Soc.

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“…Some silanol groups usually remain uncovered and some of the support stays exposed to alkaline mobile phase attack. Thus, the chemical stability of these stationary phases is not significantly better than that of the bonded phases [24,25]. Stationary phases with improved chemical stability in high-pH mobile phases have been obtained with polymer coatings of alternative oxide particles [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Immobilized polymeric stationary phases using metalized silica supports

Faria

Jardim

Collins

2006

J of Separation Science

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Immobilized presynthesized polymers on porous metalized (zirconized or titanized) silica particles as new stationary phases with improved chemical stability for RP-HPLC are reviewed. The preparations using different polymers, such as poly(methyloctylsiloxane), poly(methyltetradecylsiloxane), and poly(butadiene), different immobilization steps (gamma radiation, thermal treatment, and microwave radiation), and the chromatographic performances of these phases for polar, apolar, acidic, and basic compounds are discussed. The stability of some of these stationary phases using alkaline mobile phases is also presented.

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“…The mobile phase elution at high pH and high salt concentration promotes fast degradation of the material, resulting in a rapid stability test and a low consumption of solvent. 6,32 The bare silica and aluminized silica supports were accurately measured before and after each elution step with the alkaline mobile phase. The percentages of remaining mass of bare silica and aluminized silica after passing 500 mL of the alkaline mobile phase were 48.9 ± 2.7 and 99.7 + 0.2%, respectively.…”

Section: Stability Evaluationmentioning

confidence: 99%

New Support for High-Performance Liquid Chromatography Based on Silica Coated with Alumina Particles

2014

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A new material based on silica coated with alumina nanoparticles was proposed for use as a chromatographic support for reversed-phase high-performance liquid chromatography. Alumina nanoparticles were synthesized by a sol-gel process in reversed micelles composed of sodium bis(2-ethylhexyl)sulfosuccinate, and the support material was formed by the self-assembly of alumina layers on silica spheres. Spectroscopic and 29 Si nuclear magnetic resonance results showed evidence of chemical bonds between the alumina nanoparticles and the silica spheres, while morphological characterizations showed that the aluminized silica maintained the morphological properties of silica desired for chromatographic purposes after alumina incorporation. Stability studies indicated that bare silica showed high dissolution (~83%), while the aluminized silica remained practically unchanged (99%) after passing one liter of the alkaline mobile phase, indicating high stability under alkaline conditions. The C18 bonded aluminized silica phase showed great potential for use in high-performance liquid chromatography to separate basic molecules in the reversed-phase mode.

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Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability

Cited by 23 publications

References 32 publications

State-of-the-art in immobilized polymer stationary phases for high-performance liquid chromatography

State-of-the-art in immobilized polymer stationary phases for high-performance liquid chromatography

Immobilized polymeric stationary phases using metalized silica supports

New Support for High-Performance Liquid Chromatography Based on Silica Coated with Alumina Particles

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