Rapid detection of four mycotoxins in corn using a microfluidics and microarray-based immunoassay system

Chen, Yiqi; Meng, Xiangrui; Zhu, Yunzeng; Mengyu, Shen; Lü, Ying; Cheng, Jing; Xu, Youchun

doi:10.1016/j.talanta.2018.04.064

Cited by 35 publications

(23 citation statements)

References 20 publications

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“…In contrast, our assays implemented on microfluidic biochips overcome these shortages by being automated and more than 14 times faster. More recently, Chen et al (2018) successfully developed an automated microfluidic microarray to detect four mycotoxins. Detection of T-2 and AFB1 was achieved in 30 min with a prototyping non-portable fluorescence detection platform providing slightly better LODs than the ones reported here.…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

et al. 2019

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Phycotoxins and mycotoxins, such as paralytic shellfish poisoning toxins, type A trichothecenes, and aflatoxins are among the most toxic low molecular weight toxins associated with human poisoning incidents through the consumption of naturally contaminated food. Therefore, there is an utmost need for rapid and sensitive on-site detection systems. Herein, an electrochemical biochip for fast detection of saxitoxin, T-2 toxin as well as aflatoxin M1 and their corresponding congeners, respectively, using a portable and fully automated detection platform (pBDi, portable BioDetector integrated) was developed. Toxin analysis is facilitated upon the biochip via an indirect competitive immunoassay using toxin-specific antibodies combined with anti-idiotypic antibodies. The developed biochips enable detection in the low ng/mL-range within 17 min. Moreover, the assays cover a wide linear working range of 2–3 orders of magnitude above the limit of detection with an inter-chip coefficient of variation lower than 15%. The broad specificity of the employed antibodies which react with a large number of congeners within the respective toxin group allows efficient screening of contaminated samples for the presence of these low molecular weight toxins. With respect to the analysis of human urine samples, we focused here on the detection of saxitoxin, HT-2 toxin, and aflatoxin M1, all known as biomarkers of acute toxin exposure. Overall, it was proved that the developed biochip assays can be used to rapidly and reliably identify severe intoxications caused by these low molecular weight toxins.

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Section: Discussionmentioning

confidence: 99%

Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

et al. 2019

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“…Chen et al, have developed a simultaneous detection strategy of four mycotoxins, i.e. T2-toxin, AFB 1 , ochratoxin A, Zearalenone based on microfluidic and protein three-layered microarray consisting of PMMA (3 mm), adhesive (0.1 mm) and glass layer (1.1 mm) (Chen et al 2018). Microfluidic based AFB 1 sensor works in the range 0.12-7.19 ng/mL and detection limit of 0.03 ng/mL and limit of quantification of 0.12 ng/mL.…”

Section: -D Electrochemical Biosensor Voltammetry Biosensormentioning

confidence: 99%

Recent advances in nanomaterials integrated immunosensors for food toxin detection

2021

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For the management and prevention of many chronic and acute diseases, the rapid quantification of toxicity in food and feed products have become a significant concern. Technology advancements in the area of biosensors, bioelectronics, miniaturization techniques, and microfluidics have shown a significant impact than conventional methods which have given a boost to improve the sensing performance towards food analyte detection. In this article, recent literature of Aflatoxin B 1 (AFB 1 ), worldwide permissible limits, major outbreaks and severe impact on healthy life have been discussed. An improvement achieved in detection range, limit of detection, shelf-life of the biosensor by integrated dimensional nanomaterials such as zero-dimension, one-dimension and two-dimension for AFB 1 detection using electrical and optical transduction mechanism has been summarized. A critical overview of the latest trends using paper-based and micro-spotted array integrated with the anisotropic shape of nanomaterials, portable microfluidic devices have also been described together with future perspectives for further advancements.

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“…To overcome these shortcomings, microfluidic technology can be used for the automation and integration of multistep operations by using small cassettes, chips or disks. Several microfluidic platforms [36][37][38][39] have been developed to reduce the detection time and manual operations. On the other hand, protein microarrays have been tested in our previous studies to monitor and quantify multiple targets in human serum and urine [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers

Mengyu

2021

Micromachines

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Protein biomarkers are indicators of many diseases and are commonly used for disease diagnosis and prognosis prediction in the clinic. The urgent need for point-of-care (POC) detection of protein biomarkers has promoted the development of automated and fully sealed immunoassay platforms. In this study, a portable microfluidic system was established for the POC detection of multiple protein biomarkers by combining a protein microarray for a multiplex immunoassay and a microfluidic cassette for reagent storage and liquid manipulation. The entire procedure for the immunoassay was automatically conducted, which included the antibody–antigen reaction, washing and detection. Alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA125) were simultaneously detected in this system within 40 min with limits of detection of 0.303 ng/mL, 1.870 ng/mL, and 18.617 U/mL, respectively. Five clinical samples were collected and tested, and the results show good correlations compared to those measured by the commercial instrument in the hospital. The immunoassay cassette system can function as a versatile platform for the rapid and sensitive multiplexed detection of biomarkers; therefore, it has great potential for POC diagnostics.

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Rapid detection of four mycotoxins in corn using a microfluidics and microarray-based immunoassay system

Cited by 35 publications

References 20 publications

Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

Recent advances in nanomaterials integrated immunosensors for food toxin detection

A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers

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