Rapid and selective separation for mixed proteins with thiol functionalized magnetic nanoparticles

Abstract

Thiol group functionalized silica-coated magnetic nanoparticles (Si-MNPs@SH) were synthesized for rapid and selective magnetic field-based separation of mixed proteins. The highest adsorption efficiencies of binary proteins, bovine serum albumin (BSA; 66 kDa; pI = 4.65) and lysozyme (LYZ; 14.3 kDa; pI = 11) were shown at the pH values corresponding to their own pI in the single-component protein. In the mixed protein, however, the adsorption performance of BSA and LYZ by Si-MNPs@SH was governed not only by pH but also by the molecular weight of each protein in the mixed protein.

3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by <em>Opisthorchis felineus</em>

“…[17][18][19] One of the most effective methods of modification is the covalent modification of MNP surface using various alkoxysilane reagents. 20 Alkoxysilanes are commercially available reagents that contain various functional groups such as amino, [21][22][23][24][25][26][27][28] mercapto, [26][27][28][29] glycidyloxy, 26,30,31 cyano, 27,32 carboxylic, aldehyde, and isocyanate 27 groups. Following the modification, alkoxysilanes form a cross-linked silicone layer with functional groups covering the surface of nanoparticles.…”

Plasma polymerization of sulfur-rich and water-stable coatings on silica particles

“…The slight mass of adsorbed water was found to be related to the filter paper placed at the bottom of the test tube. As suggested by both XPS and ToF-SIMS results, the highly hydrophobic behaviour of PPT-coated particles is mainly due to the presence of nonpolar hydrocarbon (−C x H y ) functionalities [23] and also thiol (− SH) groups [5] in the structure of PPT film. From the water stability and hydrophobicity test results, it can be concluded that the produced sulfur-rich particulate surfaces are highly stable in aqueous media.…”

“…Among surface functionalities introduced on particles, sulfur-containing groups are of particular interest for the encapsulation of nanoparticles, protein separation, and water treatment [5][6][7]. The efficient removal of heavy metals such as Hg, Cd, and Pb via sulfur-functionalized particles has been well documented in the literature [7][8][9][10].…”

“…Such a wet-chemistry process is however complex and highly time consuming with stirring times as long as 16-72 h in organic solvents [11,14,15]. The wet-chemistry process also has low efficiency due to the low sulfur atomic concentrations obtained on the surface as well as inhomogeneous distribution of sulfur-containing functionalities [5,16]. More importantly, the silane chemistry method is highly substrate dependent and therefore only applicable for the modification of particles with a high concentration of silanol (− Si-OH) groups.…”

Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles

“…Compared to conventional separation, the advantages of magnetic separation are attributed to its speed, accuracy, and simplicity [3]. The surface of Fe 3 O 4 nanoparticles can be modified with functional molecules such as silane compounds and crosslinkers such as thioglycolic acid, oleic acids and N,N′-methylenebisacrylamide for enhancing their stability, dispersity and adjusting surface properties like hydrophilicity [4][5][6].…”