Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles

Maltaş, Esra; Özmen, Mustafa

doi:10.1016/j.msec.2015.05.007

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…A broad scale of materials such as chitosan, sporopollenin, magnetic nanoparticles and polymers by different functional groups have been widely used in drug delivery systems [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] . Our group has focus on development of drug carrier systems having proteins [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] . One of our previous studies has been on immobilization of hemoglobin onto sporopollenin which was showed to be high affinity to the protein, so it is possible to develop a new drug delivery system based on sporopollenin-protein [14] .…”

Section: Resultsmentioning

confidence: 99%

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Development of doxorubicin loading platform based albumin-sporopollenin as drug carrier

Maltaş

Gübbük

Yıldız

2016

Biochemistry and Biophysics Reports

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Albumin is thought as an drug carrier for doxorubicin (DOX). The binding of doxorubicin to albumin was studied on the surface of sporopolleninin (SP) to produce a new drug system based natural materials. Human serum albumin (HSA) was immobilized on SPIONs in 20 mM Tris buffer, 7.4 of pH. Data showed that binding amount of HSA has been found to be as 285.53 µg to the 25 mg of Sporopolleninin which also bounded 319.76 µM of DOX. Binding of protein and drug to Sp were clarified by SEM, EDX and FT-IR analysis.

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Section: Resultsmentioning

confidence: 99%

“…1 mM of doxorubicin was dissolved in mL of 20 mM Tris–HCl buffer (pH 7.4) [26] . The DOX solution was added to the 5–25 mg of protein immobilized sporopollenin in 20 mM Tris–HCl buffer (pH 7.4) in order to measure DOX loading.…”

Section: Methodsmentioning

confidence: 99%

Development of doxorubicin loading platform based albumin-sporopollenin as drug carrier

Maltaş

Gübbük

Yıldız

2016

Biochemistry and Biophysics Reports

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“…Excitation and emission slit widths were kept at 10.0 nm. Molecular docking was carried out using Autodock Vina (Maltas and Ozmen, 2015). …”

Section: Methodsmentioning

confidence: 99%

“…On entering the body, drugs can interact with hemoglobin affecting their distribution and efficacy, and also forming the basis of potential toxicity (Messori et al, 2006). Hemoglobin has also been extensively used for studies in protein-ligand interactions with molecules for studies in toxicity and safety (Basu and Kumar, 2015; Fennell et al, 2015; Liu and Liu, 2015; Maltas and Ozmen, 2015). …”

Section: Introductionmentioning

confidence: 99%

A Study of the Interaction of Bovine Hemoglobin with Synthetic Dyes Using Spectroscopic Techniques and Molecular Docking

2017

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Synthetic dyes are a very efficient class of dyes that are ingested or come into contact with the skin from numerous sources (cosmetics, textiles, leather, paper, and drugs). An important component of their safety profile is the interactions that they form after they enter the body. Hemoglobin is a functionally important protein that can form multiple interactions with soluble compounds present in the blood, and hence forms an important aspect of the toxicological or safety profile of the dyes. Here we study the interaction between bovine hemoglobin and organic dyes using UV-Vis absorbance and fluorescence spectroscopy. Molecular modeling was used to visualize the binding site and partners of the dye molecules, within the hemoglobin molecule. We find that all four dyes studied form sufficiently strong interactions with hemoglobin to allow for the formation of potentially toxic interactions. Molecular modeling showed that all four dyes bind within the central cavity of the hemoglobin molecule. However, binding partners could not be identified as multiple binding conformations with very similar energies were possible for each dye.

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“…In recent years, considerable effort has been focused on improving the control of size, shape, and surface modifications to gain better behavior as well as improved functionalization. Magnetic nanoparticles (NPs) have become widely studied due to the rapid increase in the number of applications in the biomedical field, such as magnetic resonance imaging [1], hyperthermia [2], drug delivery [3,4], gene therapy [5,6], protein immobilization [7,8], and immunoassay [9]. Among other applications of magnetic NPs, for the immunoassay, the conjugation of NPs with antibodies is the critical step and severely limited by low efficiency of antibodies grafted on the NP surface.…”

Section: Introductionmentioning

confidence: 99%

Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating

Villegas-Serralta

Zavala

García-Casillas

et al. 2018

Journal of Nanomaterials

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Considerable effort has been focused on improving the control of size, shape, and surface modifications to detect proteins. The purpose of this study was to compare the efficiencies of aminosilane-coated magnetite (As-M) nanoparticles (NPs), dextran-coated magnetite nanoparticles (Dx-M), and bare nanoparticles for conjugating single-chain variable fragment antibodies (scFvs) with the aim of detecting the human epidermal growth factor receptor 2 (HER2) protein. Dx-M and As-M NPs were characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Raman spectroscopy. Dx-M and As-M were conjugated with a monoclonal scFv for active targeting of the HER2 antigen. Aminosilane surface coating enhanced the scFv conjugation efficiency over twofold compared to that of the dextran-coated magnetite NPs for the detection of HER2 proteins.

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Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles

Cited by 23 publications

References 28 publications

Development of doxorubicin loading platform based albumin-sporopollenin as drug carrier

Development of doxorubicin loading platform based albumin-sporopollenin as drug carrier

A Study of the Interaction of Bovine Hemoglobin with Synthetic Dyes Using Spectroscopic Techniques and Molecular Docking

Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating

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