Rapid and highly sensitive detection of pyocyanin biomarker in differentPseudomonas aeruginosainfections using gold nanoparticles modified sensor

Abstract

Successful antibiotic treatment of infections relies on accurate and rapid identification of the infectious agents. Pseudomonas aeruginosa is implicated in a wide range of human infections that almost complicated and become life threating especially in immunocompromised and critically ill patients. Conventional microbiological methods take more than 3 days to obtain accurate results. Pyocyanin is a distinctive electroactive biomarker for Pseudomonas aeruginosa.Here, we have developed a rapid diagnostic (polyaniline) PANI gold nanoparticles (Au NPs) modified indium tin oxide (ITO) electrode that showed 100% sensitivity for pyocyanin in culture of Pseudomonas aeruginosa clinical isolates and high selectivity for pyocyanin at low concentration when measured in the presence of other substances like ascorbic acid, uric acid, and glucose as interferences. The constructed electrode was characterized using scanning electron microscopy and cyclic voltammetry. The determined linear range for pyocyanin detection was from 238 µM to 1.9 µM with a detection limit of 500 nM. Compared to the screenprinted electrode used before, the constructed electrode showed a 4-fold enhanced performance.

Electrochemical Detection of Pyocyanin as a Biomarker for Pseudomonas aeruginosa: A Focused Review

“…Several sensor designs have been described in the literature, ranging from clean-room fabricated nanograss sensors, conventional rod-electrode setups to cheaper models screen-printed on paper [ 23 , 31 , 32 ]. The fabrication techniques included shadow-printing, ink-jet printing, atomic layer deposition, electrodeposition and electron-beam deposition, while the electrodes where modified with nanoparticles, carbon-nanotubes and nanoalloys in addition to biological modifications to optimize the sensitivity and detection limit [ 29 , 30 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ].…”

Emerging Technologies for the Electrochemical Detection of Bacteria

“…[ 68 ] PANI/Au nanostructures modified ITO sensor has been designed for early detection and quantification of PYO. [ 71 ] A wide range of PYO concentrations from 238 to 1.9 µ m has been tested, and the CV technique has been used to assess the selectivity of the electrode ( Figure 7 ). [ 71 ] The composite biosensor showed high selectivity toward PYO in the presence of urea, glucose, and vitamin c and a low LOD of about 500 n m .…”

“…[ 71 ] A wide range of PYO concentrations from 238 to 1.9 µ m has been tested, and the CV technique has been used to assess the selectivity of the electrode ( Figure 7 ). [ 71 ] The composite biosensor showed high selectivity toward PYO in the presence of urea, glucose, and vitamin c and a low LOD of about 500 n m . The applicability of this sensor has been further confirmed in culture of clinical isolates obtained from cases of PA infections.…”