Rapid analysis of N-methylpyrrolidine in cefepime with thermal desorption ion mobility spectrometry

Reynolds, James C.; Giddings, Laura; Usen, Ime C.; Cheng, Haodong; Shan, Junmin; Turner, Matthew A.; Creaser, Colin S.

doi:10.1007/s12127-016-0210-7

Cited by 2 publications

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References 30 publications

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“…Cefepime chemically 7--(2-aminothiazol-4-yl)-(z)methoxyiminoacetamido]-3-(1methylpyrrolidino)-methyl-3-cephem-4-carboxylate [1], as shown in figure 1. CFM is a third-generation cephalosporin bactericidal activity spectrum in vivoandin vitroagainstaerobic gram-negative and gram-positive micro-organisms [2,3], including penicillin resins, For the analysis of Tazo-bactam (TZB) in pharmaceutical preparations [4,5] and plasma [6] either alone or in combination with other products, various analytical methods such as spectrophotometry [7,8]HPLC8 [9] TLC [10] exist for the analysis of cefepime (CFM) and similarly different HPLC methods are available. Diazotization method inexpensive and very simple method, the Azo-dye it is very important families of dye, that contain -N=Nband [11,12].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Estimation of Cefepime by using Batch, Cloud Point extraction and Flow Injection Analysis methods

2021

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New simple sensitive spectrophotometric methods are developed for determination of cefepime (CFM) in pure and Pharmaceutical Formulations. The first method conversion primary amine to azo-dye by react cefepime with sodium nitrite and hydrochloric acid followed by coupling with 2,6-dimethylphenol in alkaline medium to obtain a stable reddish-orange colored dye at λ max 515nm. The concentration ranges 2-50 μg / mL, Beer's law is obeyed, correlation coefficient was 0.9996, molar absorptivity was 0.826×10 4 L.mol -1 .cm -1 and the detection limit was 0.192 μg/mL. Cloud point extraction (CPE) second method to estimation a trace amount in aqueous solution product from diazotization and measuring with a UV-visible spectrophotometer at λ max 515 nm. The concentration range obeyed the Beer's law was 0.25-10 μg / mL, correlation coefficient was 0.9997, molar absorptivity was 1.169×10 5 L.mol -1 .cm -1 , detection limit was 0.027 μg/mL, Pre-concentration factor was 25 and Distribution coefficient(D) was 332.35. Last methods flow injection analysis it's simple for estimation the cefepime .The concentration ranges 1-150 μg / mL, Beer's law is obeyed, correlation coefficient was 0.9997, molar absorptivity was 0.341×10 4 L.mol -1 .cm -1 and the detection limit was 0.464μg/mL. The proposed methods were successfully, applied to the determination cefepime in pharmaceutical formulation.

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Section: Introductionmentioning

confidence: 99%