Quantitative trait loci associated with longevity of lettuce seeds under conventional and controlled deterioration storage conditions

Abstract

Lettuce (Lactuca sativa L.) seeds have poor shelf life and exhibit thermoinhibition (fail to germinate) above ∼25°C. Seed priming (controlled hydration followed by drying) alleviates thermoinhibition by increasing the maximum germination temperature, but reduces lettuce seed longevity. Controlled deterioration (CD) or accelerated ageing storage conditions (i.e. elevated temperature and relative humidity) are used to study seed longevity and to predict potential seed lifetimes under conventional storage conditions. Seeds produced in 2002 and 2006 of a recombinant inbred line (RIL) population derived from a cross between L. sativa cv. Salinas×L. serriola accession UC96US23 were utilized to identify quantitative trait loci (QTLs) associated with seed longevity under CD and conventional storage conditions. Multiple longevity-associated QTLs were identified under both conventional and CD storage conditions for control (non-primed) and primed seeds. However, seed longevity was poorly correlated between the two storage conditions, suggesting that deterioration processes under CD conditions are not predictive of ageing in conventional storage conditions. Additionally, the same QTLs were not identified when RIL populations were grown in different years, indicating that lettuce seed longevity is strongly affected by production environment. Nonetheless, a major QTL on chromosome 4 [Seed longevity 4.1 (Slg4.1)] was responsible for almost 23% of the phenotypic variation in viability of the conventionally stored control seeds of the 2006 RIL population, with improved longevity conferred by the Salinas allele. QTL analyses may enable identification of mechanisms responsible for the sensitivity of primed seeds to CD conditions and breeding for improved seed longevity.

Biochemical changes in fiber naturally colored cottonseeds during storage

“…These results confi rm the assumption that the storage period affects seed deterioration by causing a reduction in reserve compounds, a phenomenon that has also been observed in other oilseed species, such as soybean and sunfl ower (Balešević-Tubić et al, 2005, Sharma et al, 2011. In general, there was a decrease in the concentration of fatty acids with the progress of seed aging, with the most marked decreases in unsaturated fatty acids such as oleic ( Figure 7C) and linoleic fatty acids ( Figure 7D).…”

“…Based on these results can be affi rmed that the rate of deterioration depends on initial seed physiological quality and environmental conditions (Walters et al, 2010). When seeds are kept at higher temperatures, deterioration is more intense due to lipid peroxidation, which cause damages in cellular membrane system, thus reducing seed viability and vigor (Schwember and Bradford, 2010). According with Lins et al (2014) the deterioration of oil seeds can occur when the seed mass is heated.…”

Dormancy and enzymatic activity of rice cultivars seeds stored in different environments

“…In the storage conditions, temperature and relative humidity are key factors in maintaining seed quality (Tonin and Perez, 2006), influencing the speed of the biochemical processes and indirectly interfering in water content of seeds. The process of deterioration in seeds comprises a sequence of biochemical and physiological changes initiated immediately after physiological maturity, which lead to reduced vigor, culminating in the loss of germination capacity.…”

Electrospray Facilitates the Germination of Plant Seeds

“…The shelf life test was performed on lettuce seeds that had undergone the NP electrospray treatment, in order to evaluate the practical potential of the proposed method (Schwember and Bradford, 2010). Aged lettuce seeds treated by NP electrospray were sealed and stored in the dark and at room temperature for various time periods (i.e., 1 day, 1 week, and 1 month) prior to incubation.…”