Quantitative polymerase chain reaction‐based detection of HPV 16 E6 and E7 DNA in oral squamous cell carcinoma

Thammaiah, Smitha; Venkobarao, Mohan C.; Hemavathy, S; Mirnalini, Angeline S.

doi:10.1111/jop.12770

Cited by 9 publications

(6 citation statements)

References 26 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the TSCC cell line is the most common oral cancer and the site with the highest HPV infection rate in oral cancer, it is necessary to establish this type of cell line. But TSCC cell lines transfected with HPV16 E6E7 were inexistent in the decades, which was really a developmental limitation for a further study on the pathological mechanism, preventive measures, and treatment protocols of oral cancers, with the finding that E6 and E7 showed increasing expression as the HPV16 infection increased [ 27 ]. In this study, we transfer the pEGFP/HPV16 E6E7 and the pEGFP/no HPV16 E6E7 into TSCC cells separately, to establish a stable HPV-transferred TSCC cell line and further confirm the possible role of E6 and E7.…”

Section: Discussionmentioning

confidence: 99%

Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

Lan

Jia

Guo

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Background. Human tongue squamous cell carcinoma (TSCC) is the most common oral cancer with the highest human papillomavirus (HPV) infection rate in oral cancer. The purpose of this study was to research the correlation between HPV and TSCC. Method. Plasmid pEGFP/HPV16 E6E7 and plasmid pEGFP/no HPV16 E6E7 were constructed. TSCC cell lines SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic. Fluorescence imaging, PCR, and Western blot were used to detect the expression of HPV16 E6E7 in cells. The biological characteristics were detected by CCK-8, wound healing assay, qRT-PCR, and Western blot. Result. TSCC cell lines transfected with HPV16 E6E7 gene were successfully established and identified. And the proliferation and migration ability of the TSCC cell lines infected with HPV16 E6E7 gene were significantly stronger than that of the blank group. Conclusion. TSCC cell lines infected with HPV16 E6E7 with significantly higher ability of proliferation and migration were more malignant than those not infected with HPV16 E6E7.

show abstract

Section: Discussionmentioning

confidence: 99%

Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

Lan

Jia

Guo

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Whether and how HPV plays an effective regulatory role in changing the survival rate of human oral squamous cell carcinoma patients has not been clari ed [11,12]. However, the detection of biomarkers for the sensitivity and speci city of human oral squamous cell carcinoma associated with HPV has been compared and clari ed in this process, among which HPV16, E6 and E7 have been studied and discussed most frequently [13,14]. Multiple pathogenic factors and signaling pathways such as P16, P53, RB, E6, E7 and EGFR were studied in clinical cases and experimental observations [15].…”

mentioning

confidence: 99%

WITHDRAWN: Model of human tongue squamous cell line stably transfected with human papillomavirus（HPV) 16 E6 and E7 genes and biological characteristic analysis

Lan

Jia

Guo

et al. 2020

Preprint

View full text Add to dashboard Cite

Human tongue squamous carcinoma cell lines transfected with HPV16E6E7 gene were established to provide a model for further study of HPV16 E6E7-related human tongue squamous carcinoma cell lines .Plasmid pEGFR/HPV16 of E6E7 and plasmid pEGFR/HPV16 of No E6E7 were constructed.Human tongue squamous carcinoma cell lines including SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic .Fluorescence imaging, RT-PCR and Westernblot were used to detect the expression of HPV16 E6E7 in cells.The biological characteristics of human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were detected by CCK-8 and wound healing assay. The human tongue squamous carcinoma cell lines transfected with HPV16 E6E7 gene were successfully established and identified, and the proliferation and migration ability of the human tongue squamous carcinoma cell lines infected with HPV16 E6E7 gene was significantly stronger than that of the blank group.Human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were more malignant, and their proliferation and migration ability were higher than those not infected with HPV16 E6E7.

show abstract

“…In the comparison among p16 immunoexpression, conventional PCR, and qPCR, Thammaiah et al. (2018) highlight that qPCR is a fast, reliable, and accurate technique which provides valuable information about the infection status in terms of viral load. In the opinion of Rodríguez‐Santamarta et al.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of HPV and EBV in OSCC and the expression of p53, p16, E‐cadherin, COX‐2, MYC, and MLH1

et al. 2021

View full text Add to dashboard Cite

Objective This study aimed to evaluate the presence of human papillomavirus (HPV) and Epstein–Barr virus (EBV) and the expression of p53, p16, E‐cadherin, COX‐2, MLH1, and MYC in oral squamous cell carcinoma (OSCC). Materials and Methods One hundred OSCC specimens were submitted to in situ hybridization for HPV and EBV, and immunohistochemistry for detection of the human proteins. Results Thirty‐one cases showed HPV in tumor tissue. EBV was not detected in any case investigated. The HPV(+) group demonstrated an increase of staining scores for nuclear p16 (p = .047), cytoplasmic MYC (p = .002), while a decrease for nuclear MLH1 (p = .048), suggesting that HPV may upregulate the expression of the first two proteins and down‐regulate the latter. Conclusion Our findings reinforce the hypothesis of the HPV‐related oral carcinogenesis involving the expression of p16 and MYC, and MLH1 suppression. Exclusively cytoplasmic stainings for p16, MLH1, and MYC were also associated with more advanced tumors. Finally, in view of the lack of studies correlating the HPV or EBV infection to the expression of oncoproteins, more researches assessing a broader panel of markers and employing different approaches are still necessary in order to understand the role of these viruses as well as the molecular mechanisms involved in the development and progression of oral carcinomas.

show abstract

Quantitative polymerase chain reaction‐based detection of HPV 16 E6 and E7 DNA in oral squamous cell carcinoma

Abstract: qPCR detection of HPV infection is a fast, reliable, and accurate technique gives valuable information about the infection status in terms of viral load.

Cited by 9 publications

References 26 publications

Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

WITHDRAWN: Model of human tongue squamous cell line stably transfected with human papillomavirus（HPV) 16 E6 and E7 genes and biological characteristic analysis

Evaluation of HPV and EBV in OSCC and the expression of p53, p16, E‐cadherin, COX‐2, MYC, and MLH1

Contact Info

Product

Resources

About