Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

Abstract

Background. Human tongue squamous cell carcinoma (TSCC) is the most common oral cancer with the highest human papillomavirus (HPV) infection rate in oral cancer. The purpose of this study was to research the correlation between HPV and TSCC. Method. Plasmid pEGFP/HPV16 E6E7 and plasmid pEGFP/no HPV16 E6E7 were constructed. TSCC cell lines SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic. Fluorescence imaging, PCR, and Western blot were used to detect the expression of HPV16 E6E7 in cells. The biological characteristics were detected by CCK-8, wound healing assay, qRT-PCR, and Western blot. Result. TSCC cell lines transfected with HPV16 E6E7 gene were successfully established and identified. And the proliferation and migration ability of the TSCC cell lines infected with HPV16 E6E7 gene were significantly stronger than that of the blank group. Conclusion. TSCC cell lines infected with HPV16 E6E7 with significantly higher ability of proliferation and migration were more malignant than those not infected with HPV16 E6E7.

HPV16 E6 and E7 are involved in inhibition of autophagy and maintenance of cancer stem cell properties in HPV16‐positive oral squamous cell carcinoma cells

“…Ectopic transfection of HPV16 E6/E7 enhanced cell proliferation in tongue cancer cell lines 28 . Genetic deletion of HPV16 E7 causes inhibition of cell proliferation in HPV16‐positive oral cancer cell lines 29 .…”

“…Furthermore, 5-FU-induced LDH activity was significantly Ectopic transfection of HPV16 E6/E7 enhanced cell proliferation in tongue cancer cell lines. 28 Genetic deletion of HPV16 E7 causes inhibition of cell proliferation in HPV16-positive oral cancer cell lines. 29 In this study, we found that HPV16 E6/E7 knockdown attenuated cell proliferation in HPV16-positive UM-SCC 104 cells.…”

Retracted: Model of Human Tongue Squamous Cell Lines Stably Transfected with Human Papillomavirus (HPV)16 E6 and E7 Genes and Biological Characteristic Analysis

“…This article has been retracted by Hindawi, as publisher, following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of systematic manipulation of the publication and peer-review process.…”