Evaluation of HPV and EBV in OSCC and the expression of p53, p16, E‐cadherin, COX‐2, MYC, and MLH1

Lima, Marcos Antônio Pereira de; Cavalcante, Roberta Barroso; Silva, Cláudio Gleidiston Lima da; Nogueira, Renato Luiz Maia; Macedo, Geamberg Einstein Cruz; Galiza, Lara Eduardo de; Pinheiro, Juliana Viana; Filho, Pedro Hugo Bezerra Maia; Santos, Sarah Ferreira; Rabenhorst, Sílvia Helena Barem

doi:10.1111/odi.13814

Cited by 11 publications

(8 citation statements)

References 182 publications

(330 reference statements)

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“…Interestingly, it has been speculated that the p16 concentration could be higher in OSCC patients with HPV infection [ 53 ]. The correlation is attributed to the effect of the viral protein E7 that is able to inhibit tumour suppressors, particularly Rb1 protein, which could result in an increased p16 level [ 47 , 54 ]. Consequently, multiple studies have attempted to evaluate the utility of p16 as a surrogate marker for HPV infection in OSCC.…”

Section: Discussionmentioning

confidence: 99%

The Potential Association between E2F2, MDM2 and p16 Protein Concentration and Selected Sociodemographic and Clinicopathological Characteristics of Patients with Oral Squamous Cell Carcinoma

Świętek

Gołąbek

Hudy

et al. 2023

CIMB

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Background: E2F transcription factor 2 (E2F2), murine double minute 2 (MDM2) and p16 are some of the key proteins associated with the control of the cell cycle. The aim of this study was to evaluate E2F2, MDM2 and p16 concentrations in the tumour and margin samples of oral squamous cell carcinoma and to assess their association with some selected sociodemographic and clinicopathological characteristics of the patients. Methods: The study group consisted of 73 patients. Protein concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Results: There were no statistically significant differences in the levels of E2F2, MDM2 or p16 in the tumour samples as compared to the margin specimens. We found that patients with N0 showed significantly lower E2F2 concentrations than patients with N1 in the tumour samples and the median protein concentration of E2F2 was higher in HPV-negative patients in the tumour samples. Moreover, the level of p16 in the margin samples was lower in alcohol drinkers as compared to non-drinkers. Similar observations were found in concurrent drinkers and smokers compared to non-drinkers and non-smokers. Conclusions: E2F2 could potentially promote tumour progression and metastasis. Moreover, our results showed a differential level of the analysed proteins in response to alcohol consumption and the HPV status.

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Section: Discussionmentioning

confidence: 99%

The Potential Association between E2F2, MDM2 and p16 Protein Concentration and Selected Sociodemographic and Clinicopathological Characteristics of Patients with Oral Squamous Cell Carcinoma

Świętek

Gołąbek

Hudy

et al. 2023

CIMB

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“…Staining of tissue sections showed that FAM3B expression decreased in OSCC tissues. Mouse experiments showed that knockdown of FAM3B promotes cell apoptosis by upregulating p53 in mice [ 27 ], and the expression of p53 protein increases in OSCC [ 28 ]. Therefore, FAM3B might cause OSCC by upregulating p53 protein.…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers Associated with Cancerous Change in Oral Leukoplakia Based on Integrated Transcriptome Analysis

Chun-shen

Shi

Zuo

et al. 2022

Journal of Oncology

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Objective. Oral leukoplakia (OLK) is the most common precancerous lesion in the oral cavity. This study aimed to explore key biomarkers for monitoring OLK for early diagnosis of oral squamous cell carcinoma (OSCC) and screen small-molecule drugs for the prevention of OSCC. Method. The Gene Expression Omnibus (GEO) database was explored to extract two microarray datasets, namely, GSE85195 and GSE25099. The data of the normal group, OLK group, and OSCC group were analyzed by weighted gene coexpression network analysis (WGCNA) to identify the most significant gene module and differentially expressed genes (DEGs). The intersection genes were extracted as the key genes of OLK carcinogenesis. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed in the module. Connectivity Map and molecular docking were used to screen small-molecule drugs. The diagnostic values of four key genes were identified and verified in the GSE26549 dataset. Results. WGCNA obtained the red module (r = −0.91, p < 0.05 ) with the strongest correlation with cancerous phenotype. GO enrichment analysis showed 60 pathways, including 28 biological processes, 11 cell components, and 21 molecular functions, and KEGG enrichment analysis showed 4 pathways ( p < 0.05 ). In the differential expression analysis, there was no intersection between the upregulated genes and the red module genes. However, the intersection of the downregulated genes and the red module genes yielded 4 key genes: dopachrome tautomerase (DCT), keratin 3 (KRT3), keratin 76 (KRT76), and FAM3 metabolic regulation signal molecule B (FAM3B). The area under the curve of the diagnostic model constructed by these four genes was 0.963 (CI = 0.913–1.000). The sensitivity was 0.933, and the specificity was 0.923. The diagnostic model was successfully verified in GSE26549 (AUC = 0.745, CI = 0.638–0.851). Compared with the diagnostic models of the previous studies, the diagnostic efficiency of this model was the highest. The small-molecule drugs, selumetinib and benidipine, were selected according to the gene expression profile and showed binding activity when docking with the above molecules. Conclusions. This study provides new targets and drugs for OLK. These targets could be used as the key diagnostic molecules for long-term follow-up of OLK. The small-molecule drugs selumetinib and benidipine could be used for the prevention and treatment of OSCC.

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“…Although P53 protein is targeted by E6 and degraded in many cancers, this protein was not reduced in OSCCs in a number of studies. The justification for this event states that because of the increase in mutant p53 in HPV-negative OSCC patients, as well as the fact that this event occurs prior to HPV infection, E6 loses its ability to bind to this mutated protein and cannot degrade it [ 105 ].…”

Section: Head and Neck Cancermentioning

confidence: 99%

“…Although the results regarding the expression of P16 in HPV-positive OSCC patients are contradictory, an increase in the expression of this protein has been reported in several studies as a result of Rb suppression by E7. As a result, this increased expression suggests a strong link between HPV infection and the presence of P16, which has been proposed as a surrogate marker for HPV-HR in a number of cancer studies [ 105 – 107 ]. c-MYC and MLH1 were other genes targeted by the HPV in this cancer, which have been reported to increase and decrease their expression, respectively.…”

Section: Head and Neck Cancermentioning

confidence: 99%

“…c-MYC and MLH1 were other genes targeted by the HPV in this cancer, which have been reported to increase and decrease their expression, respectively. The increase in c-MYC expression by E7 results from the inhibition of Rb and E6 to activate telomerase, and this increase in c-MYC expression will eventually be effective in tumor progression [ 105 ]. The E2Fs factor, which is activated by the E7 oncoprotein, targets and suppresses MLH1 as a DNA repair enzyme and tumor suppressor [ 105 ].…”

Section: Head and Neck Cancermentioning

confidence: 99%

See 1 more Smart Citation

The complexity of human papilloma virus in cancers: a narrative review

Sofiani

Veisi

Rukerd

et al. 2023

Infect Agents Cancer

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Among human tumorigenic viruses, the role of Human papillomavirus (HPV) has been proven as one of the most important oncoviruses that are associated with a large number of cancers. Most cancers of the genital area such cervical and anal cancer as are caused by HPV, and in many other cancers, such as colorectal, gastric, liver, esophageal, urinary bladder, and head and neck cancers, it is considered as one of the important risk factors. Our search was conducted for published researches between 2000 and 2022 by using several international databases including Scopus, PubMed, and Web of Science as well as Google scholar. We also evaluated additional evidence from relevant published articles. It has been demonstrated that HPV can promote tumorigenesis via focusing on genes, proteins, and signaling pathways, by using E6 and E7 oncoproteins and inhibiting two crucial tumor suppressors, P53 and Rb. The following study was performed to investigate different malignant cancers under the influence of HPV infection and changes in molecular factors caused by HPV infection.

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Evaluation of HPV and EBV in OSCC and the expression of p53, p16, E‐cadherin, COX‐2, MYC, and MLH1

Cited by 11 publications

References 182 publications

The Potential Association between E2F2, MDM2 and p16 Protein Concentration and Selected Sociodemographic and Clinicopathological Characteristics of Patients with Oral Squamous Cell Carcinoma

The Potential Association between E2F2, MDM2 and p16 Protein Concentration and Selected Sociodemographic and Clinicopathological Characteristics of Patients with Oral Squamous Cell Carcinoma

Identification of Biomarkers Associated with Cancerous Change in Oral Leukoplakia Based on Integrated Transcriptome Analysis

The complexity of human papilloma virus in cancers: a narrative review

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