Quantitative Determination of Cyclosporine in Human Whole Blood by Ultra-Performance Liquid Chromatography with Triple Quadrupole Tandem Mass Spectrometry

Abstract

Cyclosporine is an immunosuppressant drug used in organ transplants or for the treatment of autoimmune diseases. We developed and validated a simple, sensitive, and specific method using UPLC-MS/MS to determine cyclosporine levels in human whole blood. MS/MS detection was performed in the positive electrospray ionization mode with multiple reaction monitoring. Cyclosporine was extracted from whole-blood samples using ascomycin as an internal standard. The mass transitions m/z 1203.49 → 1185.53 and m/z 814.71 → 796.67 were used to assay the analyte and IS. This method was validated with respect to linearity, specificity, accuracy, precision, recovery, and stability. The method exhibited a linear response from 10 to 1000 ng mL -1 with correlation coefficient values >0.99. The precision and the accuracy values were within 15%, except at the lower limit of quatification (LLOQ). Cyclosporine was stable in whole blood with no evidence of degradation. This method was successfully applied to a pharmacokinetic study of cyclosporine in healthy volunteers following oral administration.Keywords Cyclosporine, UPLC-MS/MS, human whole blood

Dopamine‐Capped Silver Nanoparticles as a Colorimetric Probe for On‐Site Detection of Cyclosporine

“…Table presents the comparison of this work with the reported methods. Although the level of detection limit of this sensor is not comparable with that of LC–MS,,, the superior advantage of this method is to detect cyclosporine with minimized sample pre‐treatment and very small size of samples. The LLOQ of present method covered well TDM measurements.…”