Simultaneous determination of cyclosporine and tacrolimus in human whole blood by ultra-high performance liquid chromatography tandem mass spectrometry and comparison with a chemiluminescence microparticle immunoassay

Mei, Shenghui; Wang, Jiaqing; Chen, Di; Zhu, Lina; Zhao, Ming; Xia, Tian; Hu, Xin; Zhao, Zhigang

doi:10.1016/j.jchromb.2018.04.028

Cited by 21 publications

(21 citation statements)

References 40 publications

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“…The recoveries were 86.8 to 92.5%; these values were similar to those published in other studies (75.7 to 112.0%) using the one-step preparation procedure (23)(24)(25)(26). The variation of ISTD-normalized MF was <5.5%; this was lower than the values estimated by previous studies (6.3 to 10.3%) (23,24). CsA was stable in human whole blood under the tested conditions, which were similar to the conditions used in previous studies (23)(24)(25)(26).…”

Section: Discussionsupporting

confidence: 90%

“…Furthermore, the LLOQ was 5 ng/ml; this was lower than that reported by other HRMS studies (24.2-28 ng/ml) (5,6), meaning higher sensitivity. The recoveries were 86.8 to 92.5%; these values were similar to those published in other studies (75.7 to 112.0%) using the one-step preparation procedure (23)(24)(25)(26). The variation of ISTD-normalized MF was <5.5%; this was lower than the values estimated by previous studies (6.3 to 10.3%) (23,24).…”

Section: Discussionsupporting

confidence: 88%

“…The variation of ISTD-normalized MF was <5.5%; this was lower than the values estimated by previous studies (6.3 to 10.3%) (23,24). CsA was stable in human whole blood under the tested conditions, which were similar to the conditions used in previous studies (23)(24)(25)(26).…”

Section: Discussionsupporting

confidence: 61%

“…AM1, AM1c and AM9 possess partial immunosuppressive activity; on the other hand, AM1 and AM9 are related to renal transplantation rejection and exhibit nephrotoxic effects (27,28). Although numerous researchers have reported the overestimation of immunoassays with regards to CsA analysis when compared with MS (23)(24)(25)(26)(29)(30)(31)(32), the potential influence of drug-to-metabolite ratios on positive bias has remained to be determined. In the present study, semiquantitative determination of the metabolites HCsA was performed by comparing the integration of the peaks, from HCsA to the area of CsA according to the calculation method of a previous study (11), as standards for the metabolites were not available.…”

Section: Discussionmentioning

confidence: 99%

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Validation of a novel UPLC‑HRMS method for human whole‑blood cyclosporine and comparison with a CMIA immunoassay

et al. 2021

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

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Validation of a novel UPLC‑HRMS method for human whole‑blood cyclosporine and comparison with a CMIA immunoassay

et al. 2021

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“…The traditional analytical methods used to measure sirolimus mainly include high-performance liquid chromatography (HPLC) 13,14 and liquid chromatography-mass spectrometry (LC-MS). 11,15,16 Most clinical laboratories use LC-MS/MS as the first choice for drug detection, due to its high sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Jiang

Zeng

et al. 2022

Analyst

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Sensitive, wide‐range and high‐throughput quantification of cyclosporine in whole blood using ultra‐performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody‐conjugated magnetic immunoassay

Watanabe

Tanaka

Ono

et al. 2021

Biomedical Chromatography

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Because either trough or peak concentration at 2 h after administration is measured in routine therapeutic drug monitoring for cyclosporine A (CyA), a quantification method with a wide-range calibration curve capable of simultaneously measuring both concentrations is required. We developed a sensitive, wide-range and highthroughput quantification method for CyA in whole blood using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and compared patients' blood CyA levels measured by UPLC-MS/MS and antibody-conjugated magnetic immunoassay (ACMIA). Whole blood samples were prepared by solid-phase extraction using Oasis HLB μElution plate. The UPLC-MS/MS assay showed excellent linearity over a wide calibration range of 5-2500 ng/mL. Within-batch accuracy and precision as well as batch-to-batch accuracy and precision fulfilled the criteria of US Food and Drug Administration guidelines. The blood CyA concentrations measured by the UPLC-MS/MS assay correlated strongly with those measured by ACMIA. A Bland-Altman plot showed a fixed error between CyA concentrations measured by the two methods, and the concentrations measured by the UPLC-MS/MS method were consistently lower than those measured by ACMIA. We have succeeded to develop a sensitive, wide-range and high-throughput quantification method for CyA in whole blood using UPLC-MS/MS. K E Y W O R D Santibody-conjugated magnetic immunoassay, cyclosporine, therapeutic drug monitoring, ultraperformance liquid chromatography-tandem mass spectrometry | INTRODUCTIONCyclosporine A (CyA) is a calcineurin inhibitor and exhibits immunosuppressive effect by inhibiting cytokines, particular interleukin-2, which are secreted by T lymphocytes (Taylor et al., 2005). Like tacrolimus, CyA is an important immunosuppressant used in various organ transplantations and autoimmune diseases (Kaufman et al., 2004). CyA has strong pharmacological activity at low doses, but its therapeutic window is narrow. Furthermore, due to the large intra-and inter-individual variability in pharmacokinetics as well as significant disposition in erythrocytes,

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Simultaneous determination of cyclosporine and tacrolimus in human whole blood by ultra-high performance liquid chromatography tandem mass spectrometry and comparison with a chemiluminescence microparticle immunoassay

Cited by 21 publications

References 40 publications

Validation of a novel UPLC‑HRMS method for human whole‑blood cyclosporine and comparison with a CMIA immunoassay

Validation of a novel UPLC‑HRMS method for human whole‑blood cyclosporine and comparison with a CMIA immunoassay

A gold-based immunochromatographic strip for the detection of sirolimus in human whole blood

Sensitive, wide‐range and high‐throughput quantification of cyclosporine in whole blood using ultra‐performance liquid chromatography coupled to tandem mass spectrometry and comparison with an antibody‐conjugated magnetic immunoassay

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