Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Zeller‐Plumhoff, Berit; Helmholz, Heike; Feyerabend, Frank; Dose, T.; Wilde, Fabian; Hipp, Alexander; Beckmann, Felix; Willumeit‐Römer, Regine; Hammel, Jörg U.

doi:10.1002/maco.201709514

Cited by 29 publications

(25 citation statements)

References 20 publications

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“…Since CT is non-invasive, sequences of images can be acquired to observe changes over time, termed timelapse μCT. For example, temporal studies are improving our understanding of biomaterial degradation in a fluidflow environment [98], water transport in plants [35] and plant root growth [99]. These techniques have also been applied to tissues that require mechanical stress for homeostasis, such as musculoskeletal tissues [25], or respond to internal pressure, such as arteries [34].…”

Section: Tracking Microstructural Changes Within a Sample Over Timementioning

confidence: 99%

X-ray computed tomography in life sciences

et al. 2020

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Recent developments within micro-computed tomography (μCT) imaging have combined to extend our capacity to image tissue in three (3D) and four (4D) dimensions at micron and sub-micron spatial resolutions, opening the way for virtual histology, live cell imaging, subcellular imaging and correlative microscopy. Pivotal to this has been the development of methods to extend the contrast achievable for soft tissue. Herein, we review the new capabilities within the field of life sciences imaging, and consider how future developments in this field could further benefit the life sciences community.

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Section: Tracking Microstructural Changes Within a Sample Over Timementioning

confidence: 99%

X-ray computed tomography in life sciences

et al. 2020

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“…Samples immersed in SBF show a significantly higher degradation depth than those degraded in DMEM+10%FBS. As the degradation layer depth for both media has been shown to equate to the degradation rate [ 12 , 48 ], this indicates a faster degradation in SBF. This may be due to the presence of proteins in DMEM+10%FBS in particular, as previously shown [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

Evaluating the morphology of the degradation layer of pure magnesium via 3D imaging at resolutions below 40 nm

Zeller‐Plumhoff

Laipple

Słomińska

et al. 2021

Bioactive Materials

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Magnesium is attractive for the application as a temporary bone implant due to its inherent biodegradability, non-toxicity and suitable mechanical properties. The degradation process of magnesium in physiological environments is complex and is thought to be a diffusion-limited transport problem. We use a multi-scale imaging approach using micro computed tomography and transmission X-ray microscopy (TXM) at resolutions below 40 nm. Thus, we are able to evaluate the nanoporosity of the degradation layer and infer its impact on the degradation process of pure magnesium in two physiological solutions. Magnesium samples were degraded in simulated body fluid (SBF) or Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) for one to four weeks. TXM reveals the three-dimensional interconnected pore network within the degradation layer for both solutions. The pore network morphology and degradation layer composition are similar for all samples. By contrast, the degradation layer thickness in samples degraded in SBF was significantly higher and more inhomogeneous than in DMEM+10%FBS. Distinct features could be observed within the degradation layer of samples degraded in SBF, suggesting the formation of microgalvanic cells, which are not present in samples degraded in DMEM+10%FBS. The results suggest that the nanoporosity of the degradation layer and the resulting ion diffusion processes therein have a limited influence on the overall degradation process. This indicates that the influence of organic components on the dampening of the degradation rate by the suppression of microgalvanic degradation is much greater in the present study.

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“…The zoom capability is of high interest for in situ experiments including the selection of the appropriate experimental methodology for understanding the degradation mechanisms of precipitation-hardenable Mg alloys in aqueous media. It has been shown previously that synchrotron-based X-ray imaging enables in situ testing in aqueous media at sufficiently high temporal resolution 35 , 36 , yet the step towards in situ imaging of degradation of Mg alloys at the sub-micron scale still remains to be taken.…”

Section: Resultsmentioning

confidence: 99%

Nanotomographic evaluation of precipitate structure evolution in a Mg–Zn–Zr alloy during plastic deformation

Zeller‐Plumhoff

Robisch

Pelliccia³

et al. 2020

Sci Rep

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Magnesium and its alloys attract increasingly wide attention in various fields, ranging from transport to medical solutions, due to their outstanding structural and degradation properties. These properties can be tailored through alloying and thermo-mechanical processing, which is often complex and multi-step, thus requiring in-depth analysis. In this work, we demonstrate the capability of synchrotron-based nanotomographic X-ray imaging methods, namely holotomography and transmission X-ray microscopy, for the quantitative 3D analysis of the evolution of intermetallic precipitate (particle) morphology and distribution in magnesium alloy Mg–5.78Zn–0.44Zr subjected to a complex multi-step processing. A rich history of variation of the intermetallic particle structure in the processed alloy provided a testbed for challenging the analytical capabilities of the imaging modalities studied. The main features of the evolving precipitate structure revealed earlier by traditional light and electron microscopy methods were confirmed by the 3D techniques of synchrotron-based X-ray imaging. We further demonstrated that synchrotron-based X-ray imaging enabled uncovering finer details of the variation of particle morphology and number density at various stages of processing—above and beyond the information provided by visible light and electron microscopy.

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Quantitative characterization of degradation processes in situ by means of a bioreactor coupled flow chamber under physiological conditions using time‐lapse SRµCT

Cited by 29 publications

References 20 publications

X-ray computed tomography in life sciences

X-ray computed tomography in life sciences

Evaluating the morphology of the degradation layer of pure magnesium via 3D imaging at resolutions below 40 nm

Nanotomographic evaluation of precipitate structure evolution in a Mg–Zn–Zr alloy during plastic deformation

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