Magnesium and its alloys have in recent years emerged as a promising alternative to titanium‐based implants for medical applications due to favorable degradation properties and good biocompatibility. The degradation of materials is currently investigated by studying different samples of the same material at different time points after degradation in a medium. This study is presenting a high‐resolution time‐lapse investigation of Mg‐2Ag in culture medium using synchrotron radiation‐based micro‐computed tomography over the course of 5 days. The design of the custom‐built corrosion cell and bioreactor are described. The computed degradation rate after 5 days is in agreement with the literature. SRµCT enables the segmentation of cracks forming in the degradation layer due to stresses and hydrogen development.
Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatgerm agglutinin (WGA) affinity separation. Two different porous supports, a silica-based material and a polymer-based material, were used for the immobilization of WGA. The prepared WGA adsorbents were characterized and process parameters of the affinity separation of fetuin were investigated and optimized. WGA was immobilized on silica and polymer supports with coupling yields of 99.6 and 99.4% respectively and amounts of coupled ligand of 7.9 and 9.2 mg of WGA/ml respectively. It has been shown that the specific capacities for fetuin were 5.1 mg/ml on WGA-silica, 1.8 mg/ml on WGA-polymer and 4.1 mg/ml on WGA-agarose. All three adsorbents proved to be suitable for the biospecific separation of fetuin. The polymer-based WGA adsorbent was successfully applied in the purification of fetuin from fetal bovine serum in a one-step separation process. The identity and purity of the isolated product was verified by SDS/PAGE. Under optimized conditions up to 21.6 mg of fetuin could be isolated from 1 ml of serum. The procedure described was designed to be easily scaled-up for the production of fetuin.
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