Quantitative changes in protein expression of cadmium‐exposed poplar plants

Abstract

Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification. Additionally, growth and other physiological parameters were monitored during the experiment. Treated plants exhibited an inhibition of growth and visual symptoms appeared after 7 days. A significant accumulation of Cd in all organs was recorded by ICP-MS analysis. A number of changes in the expression of proteins with various functions were identified; in particular a decreased abundance of oxidative stress regulating proteins, whereas pathogenesis-related proteins showed a drastic increase in abundance. Furthermore, a large number of proteins involved in carbon metabolism showed a decrease in abundance, while proteins involved in remobilizing carbon from other energy sources were upregulated. In conclusion, the negative effect of Cd could be explained by a deleterious effect on protein expression from the primary carbon metabolism and from the oxidative stress response mechanism. Accumulation of Cd in stems of poplar, coupled with a low impact of Cd on physiological parameters, promotes the use of poplar trees for phytoremediation purposes.

A Transcriptomic Network Underlies Microstructural and Physiological Responses to Cadmium in Populus × canescens

“…Furthermore, damage to chloroplast grana and mitochondrial cristae in the bark of cadmium-exposed P. 3 canescens is probably linked with degradation of membrane lipids and proteins caused by overexpression of a number of genes (Supplemental Table S3). In fact, membrane proteolysis is induced in pea and poplar leaves exposed to cadmium Kieffer et al, 2008Kieffer et al, , 2009b. These findings indicate that some transcriptional changes may result from impaired metabolism due to cadmium toxicity in P. 3 canescens bark.…”

“…Thus, it is essential to understand cadmium toxicity and detoxification in the bark of P. 3 canescens to restrict recycling of cadmium to roots, particularly in terms of using P. 3 canescens to remediate cadmium-polluted soils. Although responses of poplars to cadmium stress have been investigated at the proteomic and physiological levels (Schützendübel et al, 2002;Kieffer et al, 2008Kieffer et al, , 2009aKieffer et al, , 2009bDurand et al, 2010;He et al, 2011), the microstructural, transcriptomic, and physiological mechanisms underlying cadmium toxicity and detoxification remain unknown in the bark of poplars.…”

Proteomic analysis of saffron (Crocus sativus L.) grown under conditions of cadmium toxicity

“…CAO et al (2014) found that transcripts of V-ATPase were significantly elevated in a Cd-tolerant barley genotype but were not affected in a Cd-sensitive genotype. Kieffer et al (2008) found that pathogenesis-related proteins showed a dramatic increase in expression. In our study, these proteins were all significantly up-regulated under Cd stress.…”

Heavy Metal Toxicity in Plants: Proteomics Approach

“…Coomassie brilliant blue ve gümüş nitrat gibi boyama ajanları bitki proteomundaki ağır metal teşvikli değişimlerin analizinde sıklıkla kullanılmaktadır (Ahsan et al 2010;Lee et al 2010;Zhao et al 2011;Sharmin et al 2012 (Tonge et al 2001;Kieffer et al 2008;Evlard et al 2013;Printz et al 2013). 2-D DIGE tekniği, jeller arasında gözlenen varyasyonları azaltmakta ve üç farklı boyanın (Cy1, Cy2 ve Cy3) kullanımıyla tek bir jel üzerinde üç protein örneğinin karşılaştırılmasına olanak sağlamaktadır.…”

“…(2007), çeltik bitkilerinde 27 glukanaz geninin ekspresyon seviyesini farklı gelişim evrelerinin yanı sıra biyotik ve abiyotik faktörlere cevap olarak karakterize etmiştir. Bununla birlikte, glukanaz ekspresyonunun ağır metaller tarafında da düzenlendiği proteomik yaklaşımla gösterilmiştir (Führs et al 2008;Kieffer et al 2008;Ahsan et al 2010;Lee et al 2010). Glukanazların ağır metal cevaplarındaki rolleri tam olarak açık olmamasına karşın, genel stres cevabı olarak düzenlendiği ileri sürülmüştür ).…”