Quantitative Analysis of the Distribution of&lt;i&gt; cis&lt;/i&gt;-Eicosenoic Acid Positional Isomers in Marine Fishes from the Indian Ocean

Senarath, Samanthika; Yoshinaga, Kazuaki; Nagai, Toshiharu; Yoshida, Akihiko; Beppu, Fumiaki; Jayasinghe, Chamila; Devadawson, Chandravathany; Gotoh, Naohiro

doi:10.5650/jos.ess16155

Cited by 10 publications

(4 citation statements)

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“…The c 15‐20:1 isomer may act through suppressing adipogenesis or by inhibition of TG accumulation via decreased activity of the fatty acid synthase enzyme. The c 15‐20:1 isomer is present in small quantities in fish oil . The presence of different positional isomers even in small quantities in a mixture may exhibit synergistic effects and contributes to the functional properties or health benefits of particular compounds.…”

Section: Discussionmentioning

confidence: 99%

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Differential Effect of cis‐Eicosenoic Acid Positional Isomers on Adipogenesis and Lipid Accumulation in 3T3‐L1 Cells

Senarath

Yoshinaga²,

Nagai³

et al. 2018

Euro J Lipid Sci & Tech

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Cis‐eicosenoic acid‐rich fish oil have shown beneficial health effects toward obesity‐related metabolic dysfunctions. Six bioavailable cis‐eicosenoic acid positional isomers (c‐20:1 PIs) are examined for their effects on adipogenesis and lipid accumulation using 3T3‐L1 preadipocytes. Cells are cultured using experimental (c‐20:1 PIs‐added, 50 µM) or control (oleic acid (c9‐18:1, 50 µM) or no fatty acids) medium. The treatment of c‐20:1 PIs, significantly (p < 0.05) decreases the cellular triglyceride content compared to that of c9‐18:1. Cells absorbed c‐20:1 PIs at significantly (p < 0.05) different percentages and has altered the fatty acid metabolism. The c15‐20:1 comparatively down‐regulated the transcriptional factors for adipogenesis (peroxisome proliferative‐activated receptor gamma and CCCAT enhancer binding protein alpha) and lipogenesis (sterol regulatory element binding protein‐1); thus, show comparative anti‐adipogenic and anti‐lipogenic effect. In conclusion, c‐20:1 PIs affect differently on adipogenesis and lipid accumulation in 3T3‐L1 cells and the position of the double bond in c‐20:1 influences on their functionality. Practical applications: The control of the adipogenesis and lipid accumulation in adipocytes via improving the composition of dietary fat is useful as a prevention measure for obesity and subsequent diseases. This study demonstrates the beneficial effect of cis‐eicosenoic acid (c‐20:1) positional isomers particularly c15‐20:1 in controlling the adipogenesis and subsequent lipid accumulation in adipocytes. Thus, food rich in 20:1 positional isomers, such as fish oil, plant seeds, and nuts are useful as functional foods for the prevention of obesity and could also use as effective weight loss supplements. The effect of cis‐eicosenoic acid positional isomers (c‐20:1 PIs) on the adipogenesis and lipid accumulation is studied using 3T3‐L1 preadipocytes. Cells are cultured using experimental (c‐20:1 PIs‐added, 50 μM) or control (oleic acid (c9‐18:1, 50 μM) or no fatty acids) medium. The treatment of c‐20:1 PIs, particularly c15‐20:1 significantly (p < 0.05) decreases the cellular triglyceride content compared to that of c9‐18:1 and subsequently reduces the lipid accumulation in cells.

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Section: Discussionmentioning

confidence: 99%

“…The occurrence and distribution of c ‐20:1 PIs are significantly varied among fishes in different ocean. The predominant c ‐20:1 isomer in the Indian and Atlantic Oceanic fishes is the c 11‐20:1, while in the Pacific Ocean – fishes c 9 is the main isomer …”

Section: Introductionmentioning

confidence: 99%

Differential Effect of cis‐Eicosenoic Acid Positional Isomers on Adipogenesis and Lipid Accumulation in 3T3‐L1 Cells

Senarath

Yoshinaga²,

Nagai³

et al. 2018

Euro J Lipid Sci & Tech

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“…Fase cair bebas air diinjeksikan sebanyak 1ml untuk dianalisis dengan menggunakan alat gas kromatografi (Senarath, et al, 2017). Instrumen GC (Gas Chromatography) yang digunakan adalah RTX-5 DB-23, panjang 30 meter, diatur suhu kolom yaitu 40-250°C laju kenaikan suhu 20°C/menit, suhu detector 260°C, gas pembawa nitrogen, laju kolom 0,72ml/menit laju alir 37,7 ml/menit (Zhang, et al, 2018).…”

Section: Metode Penelitianunclassified

Analisis Kandungan Asam Lemak pada Minyak Ikan Belut

Pandiangan¹,

Panjaitan

Bangun

2021

RETIPA

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Kualitas lemak dan minyak ditentukan oleh kandungan asam lemak dan distribusinya pada molekul gliserol. Penelitian ini bertujuan untuk mengetahui kandungan asam lemak pada minyak ikan belut. Minyak ikan diambil bagian ikan yang dapat dikomsumsi, yaitu dengan diekstraksi dengan cara sokletasi menggunakan pelarut n-heksan. Setelah diperoleh minyak ikan dilakukan analisis komponen asam lemak dengan GC dan analisis karakteristik sifat fisika kimia. Hasil penelitian menunjukkan bahwa kandungan asam lemak tak jenuh lebih tinggi dibandingkan asam lemak jenuh. Total asam lemak jenuh sebesar 40,438%, total asam lemak tak jenuh sebesar 59,562% yang terdiri dari MUFA sebesar 16,298% dan PUFA sebesar 43,272%. Ditemukan asam lemak omega 3 yaitu asam linolenat, dan asam omega 6 yaitu asam linoleat. Dari analisis karakteristik sifat fisika kimia ditemukan masih dalam batas yang dipersyaratkan. Perbandingan omega 3 dan omega 6 pada minyak ikan belut masih dalam rentang syarat perbandingan yang dianjurkan. Dilihat dari kandungan asam lemak minyak ikan belut mengandung asam lemak omega 3 dan omega 6 sehingga sangat baik dikomsumsi untuk meningkatkan kesehatan manusia.

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“…Distribution of 13 isomers of c-18:1 in dietary fats and oils, with double bond positions from ∆4 to ∆16, have been reported by researchers 6 . Furthermore, six PIs of c-20:1 and five of c-22:1 were reported in marine fish lipids 7,8 .…”

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confidence: 99%

Comparison of the Effects of Long-chain Monounsaturated Fatty Acid Positional Isomers on Lipid Metabolism in 3T3-L1 Cells

et al. 2019

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Introduction Long chain monounsaturated fatty acids LC-MUFAs constitute an important group of fatty acids FAs in dietary lipids. Most of the health foods are rich with approximately 40-60 of the total fatty acid content in LC-MUFAs, particularly cis-9-octadecenoic acid c9-18:1, oleic acid. In plant-based foods, c9-18:1 is the predominant LC-MUFA 1. However, some marine fish and plants belonging to Cruciferae family are reported to have lipids with high content of cis-eicosenoic acid c-20:1 and cis-docosenoic acid c-22:1 2, 3. Thus, the total content of LC-MUFAs varies in the lipids of plants and animals, particularly fishes. A recent study conducted using different n-3 polyunsaturat

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Quantitative Analysis of the Distribution of<i> cis</i>-Eicosenoic Acid Positional Isomers in Marine Fishes from the Indian Ocean

Cited by 10 publications

References 24 publications

Differential Effect of cis‐Eicosenoic Acid Positional Isomers on Adipogenesis and Lipid Accumulation in 3T3‐L1 Cells

Differential Effect of cis‐Eicosenoic Acid Positional Isomers on Adipogenesis and Lipid Accumulation in 3T3‐L1 Cells

Analisis Kandungan Asam Lemak pada Minyak Ikan Belut

Comparison of the Effects of Long-chain Monounsaturated Fatty Acid Positional Isomers on Lipid Metabolism in 3T3-L1 Cells

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