Comparison of the Effects of Long-chain Monounsaturated Fatty Acid Positional Isomers on Lipid Metabolism in 3T3-L1 Cells

Abstract

Introduction Long chain monounsaturated fatty acids LC-MUFAs constitute an important group of fatty acids FAs in dietary lipids. Most of the health foods are rich with approximately 40-60 of the total fatty acid content in LC-MUFAs, particularly cis-9-octadecenoic acid c9-18:1, oleic acid. In plant-based foods, c9-18:1 is the predominant LC-MUFA 1. However, some marine fish and plants belonging to Cruciferae family are reported to have lipids with high content of cis-eicosenoic acid c-20:1 and cis-docosenoic acid c-22:1 2, 3. Thus, the total content of LC-MUFAs varies in the lipids of plants and animals, particularly fishes. A recent study conducted using different n-3 polyunsaturat

Valerenic Acid Promotes Adipocyte Differentiation, Adiponectin Production, and Glucose Uptake via Its PPARγ Ligand Activity

“…TG Quantification Assay. The cellular concentration of triglyceride (TG) was determined using the method of Senarath et al 45 with a few modifications. Briefly, mature adipocytes obtained on the eighth day were rinsed twice with PBS; the cells were scraped into PIPES (Piperazine-N,N′bis(2-ethanesulfonic acid)) buffer with 0.1% Triton X and lysed by sonication on ice.…”