Quantitative analysis of mebeverine in dosage forms by HPLC

Abstract

Key WordsColumn liquid chromatography Mebeverine in dosage forms SummaryA simple and rapid, high performance liquid chromatographic (HPLC) procedure for determination of mebeverine in dosage forms (tablet and liquid) is described. Reversed-Phase chromatography was carried out using a mobile phase containing 0.05 M ammonium acetate buffer and acetonitrile, [(45 %, v/v) pH 5.2] with UV-detection (263 nm). Replicate regression analyses of three standard plots in the concentration range of 0.5-10 meg mL -1 obtained on three different days gave a correlation coefficient > 0.9995 and the coefficient of variation of the slopes < 2.2 %. The assay was precise within day and between days as indicated by ANOVA test. The recoveries from 10 replicate tablets of two commercial mebeverine brands and liquid were in order 99.3,100.5 and 100.1% of the label amount and their coefficient of variations were 1.41, 0.89 and 0.69 %, respectively. The limit of quantitation of mebeverine was 5 ng mL-1.

Quantitative HPLC analysis of mebeverine, mesalazine, sulphasalazine and dispersible aspirin stored in a Venalink monitored dosage system with co-prescribed medicines

“…It has been used for the quantitative determination of mebeverine HCl [11][12][13][14] and its stability in tablets [15], for the determination of 5-aminosalicylic acid (5-ASA) [16][17][18][19][20] and the stability of 5-ASA and its metabolites in plasma [21,22], for sulphasalazine [23,24] and for aspirin [25][26][27][28][29].…”

Potentiometric flow injection analysis of mebeverine hydrochloride in serum and urine

“…The reported methods for the determination of mebeverine, in the main, are chromatographic which, in spite of their good sensitivities, are very expensive, time consuming and require special training. They include high performance liquid chromatography [3][4][5][6], online micellar electrokinetic chromatography [7], highperformance thin layer chromatography [8,9], supercriticalfluid chromatography-mass spectrometry [10], and online reversed-phase liquid chromatography-gas chromatography [11]. Other alternatives include, spectrophotometry [12][13][14][15] and first-derivative UV-spectrophotometry [16].…”

“…The formed precipitates were filtered off, washed thoroughly with distilled water, dried at room temperature and ground to a fine powders. The chemical compositions of the precipitates were found to be [Mv 4 Table 1.…”

Fiber‐Based Liquid‐Phase Micro‐Extraction of Mebeverine Enantiomers Followed by Chiral High‐Performance Liquid Chromatography Analysis and Its Application to Pharmacokinetics Study in Rat Plasma

“…7 British Pharmacopoeia described a nonaqueous titrimetric method for the determination of MEB in pure form. 8 Several spectrophotometric methods, [9][10][11][12][13] electrochemical methods, [14][15][16] and chromatographic methods [17][18][19][20][21][22][23] were reported for its determination either directly or in pharmaceutical preparations and biological fluids.…”