Quantitative analysis of benzyl isothiocyanate in Salvadora persica extract and dental care herbal formulations using reversed phase C18 high-performance liquid chromatography method

Abstract

Context:Benzyl isothiocyanate is the active antimicrobial agent in Salvadora persica (siwak) widely used in Islamic countries for oral hygiene.Aims:Quantification of benzyl isothiocyanate in the ethanol extract of S. persica and some dental care herbal formulations labeled to contain siwak.Settings and Design:Simple and sensitive high-performance liquid chromatography method was designed.Subjects and Methods:Separation was achieved on reverse phase C18 (250 mm × 4.6 mm, 5 μ) column with a mobile phase comprising acetonitrile and water (1:1). The detection was carried out at 190 nm using ultra violet-visible detector. The flow rate was kept at 1 mL/min.Results:A sharp and well-defined peak was obtained at the retention time of 9.322 ± 0.3 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response and concentration in the range of 0.5–500 μg/mL with a regression coefficient (r2) of 0.9977. The method was validated for accuracy, precision, robustness, and sensitivity. All the parameters examined met the current recommendations for the International Conference on Harmonization guidelines for method validation.Conclusions:The method was applied for the quantification of benzyl isothiocyanate in siwak extract, dental care powder, mouth wash, and toothpaste claimed to contain siwak. The developed method was found specific, simple, selective, and reliable for routine use in quality control analysis of different commercially available herbal care products.SUMMARY A simple, accurate and precise method was developed for the analysis of the antimicrobial agent benzyl isothiocyanate in Salvadora persica (Siwak) extract and selected dental care herbal formulations using RP18 HPLCAmount of benzyl isothiocyanate will indicate the efficacy of Siwak productsThe method subject to ICH validation guidelines. Abbreviations used: RP18: Reversed phase C18, HPLC: High performance liquid chromatography, UV: Ultra violet, r2: regression coefficient, ICH: international conference on harmonization, TLC: Thin layer chromatography, CHCl3: Chloroform, v/v: volume/volume, RSD: Relative standard deviation, LOD: Limit of detection, LOQ: Limit of quantification.

Optimization of the extraction condition for benzyl isothiocyanate contents in Salvadora persica roots “Siwak”

“…Our analysis of some marketed Siwak containing products revealed that none of them contain benzyl isothiocyanate (Abdel-Kader et al, 2017b, Abdel-Kader et al, 2017c, Abdel-Kader et al, 2018). In a previous study the effect of extraction solvents indicated that hydroxylated solvents such as methanol and ethanol react with benzyl isothiocyanate to form the corresponding inactive thiocarbamate derivatives (Abdel-Kader et al, 2019).…”

“…Plates were left to dry for 10 min and the Silica gel layers were covered tightly with 10 × 20 glass plates. Spots were detected under UV light at 191 nm (Abdel-Kader et al, 2017b).…”

“…The HPTLC and HPLC methods were validated for linearity, precession, accuracy, robustness, specificity, limit of detection (LOD) and limit of quantification (LOQ) following the international conference on harmonization (ICH) guide lines (ICH, 2005) as described earlier (Abdel-Kader et al, 2017b, Abdel-Kader et al, 2017c).…”

Effect of hydroxylated solvents on the active constituents of Salvadora persica root “Siwak”

“…Analysis of some marketed Siwak products recommended for dental care revealed that non of them contain benzyl isothiocyanate, the major active agent of Siwak (Abdel-Kader et al, 2017b, Abdel-Kader et al, 2017c, Abdel-Kader et al, 2018). That is most likely due to the decomposition of the active compound during extraction.…”

HPLC-PDA-MS/MS PROFILE, ANTIMICROBIAL ACTIVITY OF INSTANTLY USED MISWAK (Salvadora persica L.) ROOT VERSUS ITS AQUEOUS EXTRACT AND BENZYL ISOTHIOCYANATE QUANTIFICATION

“…The antibacterial activity of S. persica different organs and extracts was previously studied 5 . However, very little work was performed on the antiviral activity 6 and methods of analysis of BICT in S. persica 7 . The objective of this study is to apply metabolomics tools (HPLC-PDA-ESI-MS/MS) and to investigate the difference in metabolites and antimicrobial activity between instantly used powder (IUP) and aqueous extract (AE) of S. persica root; as well as to establish a simple and validated method for determination of BITC in miswak dichloromethane extract (MDE) using HPLC-UV.…”