No abstract

Bioassay-guided fractionation of a methanol extract of Albizia subdimidiata using the engineered yeast strains 1138, 1140, 1353, and Sc7 of Saccharomyces cerevisiae as the bioassay tool resulted in the isolation of the two active saponins 1 and 2; one of these, albiziatrioside A (1), is described for the first time. The structures of 1 and 2 were established on the basis of HRMS, 1D and 2D NMR spectral data, and GC--MS analysis of the sugar units. Both isolated compounds showed significant cytotoxicity against the A2780 cell line.

Temperature-dependent solubility data of bioactive compound hesperidin has not been reported in any solvent in the literature so far. Therefore, the aim of the current study was to measure the solubility of bioactive compound hesperidin in six different pure solvents namely water, ethanol, isopropyl alcohol (IPA), propylene glycol (PG), poly­(ethylene glycol)-400 (PEG-400), and 1-butanol from (298.15 to 333.15) K using the shake flask method. The experimental solubilities of hesperidin were regressed by Apelblat equation with root-mean-square deviations in the range of 6.32·10–7 to 0.184 in all solvents investigated. The correlation coefficients in pure solvents were observed in the range of 0.995 to 0.999. The mole fraction solubility of hesperidin was found to be higher in PEG-400 (6.33·10–3 at 298.15 K) and PG (5.35·10–4 at 298.15 K) as compared to water (1.47·10–7 at 298.15 K), ethanol (3.45·10–5 at 298.15 K), IPA (1.53·10–5 at 298.15 K), and 1-butanol (3.15·10–4 at 298.15 K). The data of the current study could be useful in crystallization/purification and formulation development of hesperidin in the chemical/pharmaceutical industry.