Quantitation and Ultraviolet Spectrum Identification of Buflomedil in Whole Blood and Plasma by HPLC

Rop, Pok Phak; Bresson, M.; Antoine, J.; Spinazzola, J.; Fornaris, M; Viala, Alain

doi:10.1093/jat/14.1.18

Cited by 6 publications

(2 citation statements)

References 4 publications

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“…There have been few reports for its determination in formulation and in biological fluids using gas chromatography [4][5][6] , high-performance liquid chromatography 7,8 , reversed-phase ion-pair highperformance liquid chromatography 9,10 , reversedphase high-performance liquid chromatography, which mainly concerned with poising or toxicological cases 11,12 and thin layer chromatography (TLC-densitometry) 13 . Therefore, only few spectrophotometric methods for determination of BFM in presence of its degradation products were reported 13 .…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Spectrophotometric and Chemometric Methods for Determination of Buflomedil in Presence of its Acid Induced Degradation Products

Moustafa

Salem

Hegazy

et al. 2013

Analytical Chemistry Letters

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Simple, accurate, sensitive, selective and validated UV spectrophotometric univariate and multivariate methods were developed for determination of Buflomedil (BFM) in presence of BFM degradation products (BFM Deg). The univariate methods includes mean centering of ratio spectra (MCR), ratio difference spectrophotometric method (RDSM) and H-point standard addition method (HPSAM); the first one used the amplitude at 235.7 nm, the second utilized the difference in amplitudes at 250 nm and 282 nm; while, the third method utilized the absorbance values at 247 and 282.4 nm. Linearity was obtained for the proposed methods in the ranges of 10-100 μgmL-1. The percentage recoveries were 100.06 ± 0.963, 100.17 ± 0.962 and 100.90 ± 0.667 for the three methods in a respective order. Multivariate methods involves the application of three chemometric techniques, and classical least square (CLS) principal component regression (PCR) and partial least-squares regression (PLS), which were used for the simultaneous determination of the BFM and its degradation products. The results obtained from the proposed methods, together with the reported one, were statistically compared using one-way analysis of variance (ANOVA). These methods are suitable as stability indicating methods for the determination of BFM in presence of its degradation products either in bulk powder or in pharmaceutical formulations.

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Section: Introductionmentioning

confidence: 99%

Stability Indicating Spectrophotometric and Chemometric Methods for Determination of Buflomedil in Presence of its Acid Induced Degradation Products

Moustafa

Salem

Hegazy

et al. 2013

Analytical Chemistry Letters

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show abstract

“…There have been few reports for its determination in formulation and in biological fluids using gas chromatography [2][3][4], highperformance liquid chromatography [5][6][7] and reversed-phase ionpair high-performance liquid chromatography [8,9] which mainly concerned with poising or toxicological cases. Therefore, only few methods were determined of BFM in presence of its degradation products.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Validated Chromatographic Methods for Determination of Buflomedil in Presence of its Degradation Products

Salem¹,

Moustafa²,

Hegazy³

et al. 2013

Pharmaceut Anal Acta

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Simple, sensitive, selective and precise stability-indicating thin-layer chromatographic (TLC) andhigh-performance liquid chromatographic (HPLC) methodsfor the determination of Buflomedil (BFM) in pharmaceutical tablets were developed and validatedas per the International Conference on Harmonizationguidelines. The TLC method employs aluminum TLCplates precoated with silica gel 60F 254 as the stationary phase and Butanol: ammonia: triethylamine (8:0.5:0.5, v/v/v) as the mobile phase to give compact spots for Buflomedil (R f =0.55) and its degradation product (R f =0.05), the chromatogram was scanned at 272 nm. The HPLC method utilizes a C18 column and amobile phase consisting of methanol: water: acetonitrile: triethylamine (50:30:20:0.4, v/v, pH 6.5) at a flow rate of 0.7 mL min -1 for the separation of Buflomedil (t R =3.76) and its degradation product (t R =2.117). Quantitation was achieved with UV detection at 272 nm. The methods were validated in terms of accuracy, precision, linearity, limits of detection, and limits of quantification. Buflomedil was exposed to acid hydrolysis and analyzed by the proposed methods in presence of its degradation products. As the methods could effectively separate the drugs from their degradation products, these techniques can be employed as stability-indicating methods that have been successively applied to pharmaceutical formulations without interference from the excipients.

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Rapid HPLC measurement of buflomedil in plasma in poisoning cases

Forfar‐Bares

Péhourcq²,

Jarry

2002

Biomedical Chromatography

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A rapid high-performance liquid chromatographic method for the determination of buflomedil in human plasma is described. It requires a single liquid-liquid extraction step from 1 mL of plasma with diethyl ether followed by chromatography on a Nova Pak C(18) reversed-phase column and detection by ultaviolet light. Metoclopramide was used as internal standard. The method is sensitive with a quantification limit at 500 ng/mL. It was used for the determination of buflomedil in biological fluids in poisoning cases.

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Quantitation and Ultraviolet Spectrum Identification of Buflomedil in Whole Blood and Plasma by HPLC

Cited by 6 publications

References 4 publications

Stability Indicating Spectrophotometric and Chemometric Methods for Determination of Buflomedil in Presence of its Acid Induced Degradation Products

Stability Indicating Spectrophotometric and Chemometric Methods for Determination of Buflomedil in Presence of its Acid Induced Degradation Products

Stability Indicating Validated Chromatographic Methods for Determination of Buflomedil in Presence of its Degradation Products

Rapid HPLC measurement of buflomedil in plasma in poisoning cases

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