Buflomedil, a vasodilating agent, was determined in whole blood or plasma by HPLC with papaverine as internal standard after absorption of the alkaline sample on an Extrelut column and elution with diethylether-methylene chloride (70:30, v/v). The eluate was evaporated and the residue was dissolved in 100 microL of the mobile phase; 20 microL of this solution were injected into a mu Bondapak C18 column (10 microns) using acetonitrile-0.125M potassium dihydrogen phosphate (40:60, v/v) as mobile phase and UV detection at 280 nm, followed by UV spectrum identification (between 200 and 350 nm) with a photodiode array detector. The method is rapid (giving response within 20 min), reproducible, selective, and sensitive. It can be applied for pharmacokinetic studies and for both clinical pharmacology and forensic toxicology.
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