Quantification of Curcuminoids in Turmeric Using Visible Reflectance Spectra and a Decision-Tree Based Chemometric Approach

Abstract

For quantification of curcumin content in turmeric, a low-cost multivariate-analysis-based sensing system is desired. It can be realized by exploiting the spectra in the visible region, which enables the use of off-the-shelf, relatively inexpensive light sources and detectors. To address this, we propose a novel decision-tree method for improved prediction accuracy. Two sets of models with PLSR algorithm are developed with the measured reflectance spectra from 66 turmeric samples in the range of 360–750 nm, and their respective curcuminoids content are quantified by HPLC. A suite of a coarse-model for initial prediction of turmeric samples in the broad range of 1%–4%, and five finer-models for subsequent prediction (in the ranges 1%–2%, 2%–3%, 3%–4%, 1.5%–2.5%, and 2.5%–3.5%) constitute the proposed decision-tree approach. The method’s efficacy is substantiated from an improved coefficient of determination (R 2) for the finer models (0.90–0.96) as compared to the coarse-model’s 0.92. This is further corroborated with lower RMSECV of 0.06–0.13 and an RMSEP of 0.15–0.25 for finer models, as compared to 0.219 and 0.45 for the coarse model, respectively. Testing reveals that the method results in 46% reduction in prediction error. Realization of a robust prediction approach in the visible range sets the stage for the development of cost-effective field-deployable devices for on-site measurement of curcumin.

Optimization and Validation of HPLC-DAD Method for the Quantification of Curcuminoids in Turmeric (Curcuma Longa L.)

“…To determine curcumin and its derivatives in turmeric, several methods are used. These methods include UV-Vis, HPLC, LC/ MS, and the spectrofluorimetric procedure [6][7][8][9][10][11][12][13]. Among them, the most used technique is HPLC due to its high separation capability compared to the other methods [2,10,[12][13][14].…”