Quality Standards and Antioxidant Activity of Siddha Formulation Cuvacakutori Chooranam

Abstract

Objectives: To evaluate quality standards and to screen antioxidant activity of Cuvacakutori Chooranam (CC) a poly herbomineral Siddha formulation. Methods: The drug was prepared with authenticated ingredients. Powder microscopic, phytochemical, physicochemical, HPTLC fingerprint profiling (n-hexane, successive chloroform, successive ethanol), total phenol and flavonoid estimation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and superoxide scavenging activities of CC were studied. Results: Microscopic studies revealed diagnostic features to identify the formulation using microscope. Preliminary phytochemical screening revealed the presence of different secondary metabolites, unique chromatographic fingerprint and antioxidant activities. Conclusion: The powder microscopic characters and the evolved physicochemical data arrived could serve as a reference tool for quality control. The presence of different categories of phytochemicals and antioxidant activity of the drug gives broad scope for further efficacy studies of this drug.

Powder microscopic, physicochemical and chromatographic approach for the quality control of anti-hypertensive drug Rattha Piththathirku Kudinir Chooranam

“…4) of revealed 11 peaks with Rf 0.01 (area 13.59%), 0.09 (2.54%), 0.14 (0.48%), 0.17 (0.95%), 0.24 (3.96%), 0.26 (6.23%), 0.34 (0.71%), 0.52 (22.87%), 0.66 (31.70%),0.70 (13.75%) and 0.79 (3.22%) under short UV; 11 peaks with Rf 0.02 (area 48.05%), 0.08 (3.30%), 0.11 (1.18%), 0.16 (11.53%), 0.20 (1.26%), 0.59 (8.85%), 0.66 (9.52%), 0.67 (9.80%), 0.82 (5.63%), 0.96 (0.35%) and 0.97 (0.51%) under long UV and 12 peaks with Rf 0.01 (area 6.28%), 0.06 (10.88%), 0.12 (22.52%), 0.29 (6.39%), 0. (32)(33)(34).…”

Quality Assessment and Antioxidant Activities of the Blossoms ofInula NervosaWall

“…Hence, study of antioxidants of greater efficacy and lower toxicity was worthy of considerable attention. To evaluate antioxidant effects, there are many efforts spent in establishing relevant detection methods, among which 2,2 0 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferric reducing antioxidant potential (FRAP), and 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assays, were frequently employed (6)(7)(8)(9).…”