Purification of polysaccharide from Lentinus edodes water extract by membrane separation and its chemical composition and structure characterization

Tang, Wei; Liu, Cencen; Liu, Jinjin; Hu, Liuyun; Huang, Yifei; Lu, Yuan; Liu, Fangwei; Pan, Shijie; Chen, Shuping; Bian, Shuigen; Huang, Xiao‐Jun; Yin, Jingdong; Nie, Shaoping

doi:10.1016/j.foodhyd.2020.105851

Cited by 59 publications

(38 citation statements)

References 39 publications

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“…The signal at δ 4.99/98.97 ppm was attributed to α‐D‐Gal [30] and the signal at δ 5.22/102.17 ppm was considered to α‐L‐Fuc [31] . In addition, the signal peak at δ 1.23/15.91 ppm was appointed to H‐6/C‐6 of fucose, [30,32] which was in compliance with the results of monosaccharide composition determination.…”

Section: Resultssupporting

confidence: 76%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

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Section: Resultssupporting

confidence: 76%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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“…Referring to the calibration curve of dextran series standard solutions, the molecular weights of four polysaccharides (LBSDPs) were determined. Chromatographic conditions: column: BRT105-104-102 tandem gel column (8 × 300 mm); mobile phase: 0.05 M NaCl solution; and detector: RI-10A differential detector at 40 °C with a flow rate of 0.6 mL/min and injection volume of 20 µL ( Tang et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Physicochemical and antioxidant properties of Lycium barbarum seed dreg polysaccharides prepared by continuous extraction

Zhang

et al. 2022

Food Chemistry: X

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“…It could be confirmed by the results of the physicochemical properties and monosaccharide composition of CYP. The absorption peak at 1250 and 1070 might be caused by the stretching vibration of C–O [ 39 ], indicating the presence of the pyranose ring [ 40 ]. Combined with the above analysis, CYP had a clear polysaccharide characteristic.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Xiao

Xie

et al. 2022

Foods

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This study aimed to characterize the structure of Chinese yam (Dioscoreae Rhizoma) polysaccharide (CYP) and to investigate its protective effect against H2O2-induced oxidative damage in IEC-6 cells. The chemical composition and structural characteristics of the samples were analyzed by chemical and instrumental methods, including high-performance gel permeation chromatography, high-performance anion-exchange chromatography (HPAEC), Fourier transformed infrared (FT-IR), ultraviolet (UV), and scanning electron microscopy (SEM). Antioxidant activity was evaluated by establishing a cellular model of oxidative damage. The molecular weight of CYP was 20.89 kDa. Analysis of the monosaccharide composition revealed that CYP was primarily comprised of galactose (Gal), glucose (Glu), and galacturonic acid (GalA), and the ratio between them was 28.57:11.28:37.59. Pretreatment with CYP was able to improve cell viability, superoxide dismutase (SOD) activity, and reduce intracellular reactive oxygen species (ROS) production and malondialdehyde (MDA) content after H2O2 injury. CYP also attenuated oxidative damage in cells through the mitogen-activated protein kinase (MAPK) signaling pathway. This study showed that CYP was an acidic heteropolysaccharide with a good protective effect against oxidative damage, and it thus has good prospects in food and biopharmaceutical industries.

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Purification of polysaccharide from Lentinus edodes water extract by membrane separation and its chemical composition and structure characterization

Cited by 59 publications

References 39 publications

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Physicochemical and antioxidant properties of Lycium barbarum seed dreg polysaccharides prepared by continuous extraction

Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

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