The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Abstract

In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum moldavica and Their Anti‐Complementary Activity

“…The absorption peaks at 902.45 cm −1 and 812.03 cm −1 were attributed to the α ‐ and β ‐type glycosidic bond, respectively [25] . In addition, no absorption peak was found at about 1730 cm −1 , indicating that they do not contain uronic acid, which is consistent with the results of chemical composition [26] …”

“…[25] In addition, no absorption peak was found at about 1730 cm À 1 , indicating that they do not contain uronic acid, which is consistent with the results of chemical composition. [26]…”

Purification, Structural Characterization, and Antitumor Activity of a Polysaccharide from Perilla Seeds

“…The ampoules were sealed under nitrogen purge, and the samples were hydrolyzed at 120 • C for 3 h. The acid hydrolysis solution was transferred to a glass tube through aspiration, and 10 mL of deionized water was added before mixing; then, 100 µL of the solution was aspirated, and 900 µL of deionized water was added. The supernatant was centrifuged at 12,000× g for 5 min, and the monosaccharide composition of PFSP-2-1 was determined using an ion chromatograph equipped with a Dionex Carbopac TMPA20 (3 × 150) column [45].…”

A Review of Development and Utilization for Edible Fungal Polysaccharides: Extraction, Chemical Characteristics, and Bioactivities

“…In addition to autophagy, tumor cell proliferation can be inhibited by a variety of mechanisms, including cell cycle arrest, cell apoptosis, secondary necrosis, and stimulation of macrophages [ 159 ]. Niu et al [ 160 ] isolated a novel cold water-soluble polysaccharide fraction (LGP) from Leucopaxillus giganteus and found that LGP could effectively protect the thymus and spleen immune organs and inhibit the proliferation of the hepatoma H22 solid tumor in a dose-dependent manner. Further studies indicated that the antitumor mechanism of LGP may be the induced apoptosis of H22 cells through S-phase arrest and the mitochondria-mediated apoptosis pathway.…”