Isolation, Characterization and Antioxidant Activity of Yam Polysaccharides

Abstract

This study aimed to characterize the structure of Chinese yam (Dioscoreae Rhizoma) polysaccharide (CYP) and to investigate its protective effect against H2O2-induced oxidative damage in IEC-6 cells. The chemical composition and structural characteristics of the samples were analyzed by chemical and instrumental methods, including high-performance gel permeation chromatography, high-performance anion-exchange chromatography (HPAEC), Fourier transformed infrared (FT-IR), ultraviolet (UV), and scanning electron microscopy (SEM). Antioxidant activity was evaluated by establishing a cellular model of oxidative damage. The molecular weight of CYP was 20.89 kDa. Analysis of the monosaccharide composition revealed that CYP was primarily comprised of galactose (Gal), glucose (Glu), and galacturonic acid (GalA), and the ratio between them was 28.57:11.28:37.59. Pretreatment with CYP was able to improve cell viability, superoxide dismutase (SOD) activity, and reduce intracellular reactive oxygen species (ROS) production and malondialdehyde (MDA) content after H2O2 injury. CYP also attenuated oxidative damage in cells through the mitogen-activated protein kinase (MAPK) signaling pathway. This study showed that CYP was an acidic heteropolysaccharide with a good protective effect against oxidative damage, and it thus has good prospects in food and biopharmaceutical industries.

Effects of compound of hawthorn (Crataegus pinnatifida) and Chinese yam (Dioscorea opposita Thunb.) extracts on growth performance, intestinal health, and immune function in weaned pigs

“…Flavonoids may play an important role in improving antioxidant capacity, as supplementation of hawthorn‐leaves flavonoids in the diet could improve the activity of antioxidant enzymes (T‐AOC and GSH‐Px) in aged breeder hens (Dai et al, 2021). Moreover, various studies reported that the polysaccharides extracted from Chinese yam by hot water method have the effect of anti‐lipid peroxidation and scavenging hydroxyl radical and superoxide anion radicals, and their antioxidant activity reached the level of vitamin C (Li et al, 2022; Zhou et al, 2021). Antioxidant phenolic compounds extracted from hawthorn were also able to improve antioxidant capacity by increasing the expression of the antioxidant enzymes including SOD, GSH‐Px, and CAT (He et al, 2021).…”

The Molecular Mechanism of Yam Polysaccharide Protected H2O2-Induced Oxidative Damage in IEC-6 Cells

“…The dialyzed solution was lyophilized after secondary alcohol precipitation with anhydrous ethanol to obtain the crude polysaccharide [21,22]. The contents of carbohydrate, uronic acid, and protein were 33.62%, 34.95%, and 5.26%, respectively, and the molecular weight was 20.89 kDa [23].…”

“…Our preceding study has illustrated that the phosphorylated expression of three proteins, JNK, ERK, and P38, in the MAPK pathway activated by H 2 O 2 was significantly reduced in the presence of CYP, which was able to inhibit the activation of the MAPK pathway [23]. In consequence, to bear out that activating the MAPK signaling pathway in RNA-seq results, ERK inhibitor (PD98059) was added before treating the cells with H 2 O 2 .…”

Effects of dietary Chinese yam polysaccharide copper complex on growth performance, immunity, and antioxidant capacity of broilers

“…Chinese yam polysaccharide (CYP) extracted from the Chinese yam , a very popular tuber crop for food and medical purposes in China ( 1 ), has received attention in recent years, owing to its positive nutritional and medicinal characteristics ( 2 – 5 ). Previous studies have shown that the pharmacological effects of CYP include promoting growth performance, lowering blood glucose and blood lipid, and enhancing anti-oxidation and immunity in animal ( 6 – 9 ).…”