Physicochemical and antioxidant properties of Lycium barbarum seed dreg polysaccharides prepared by continuous extraction

Zhang, Xiuxiu; Ni, Zhi‐Jing; Zhang, Fan; Thakur, Kiran; Zhang, Jianguo; Khan, Mohammad Rizwan; Busquets, Rosa; Wei, Zhao‐Jun

doi:10.1016/j.fochx.2022.100282

Cited by 26 publications

(14 citation statements)

References 39 publications

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“…FT-IR spectra of LNT, LNT-SeNPs, and SeNPs were shown in Figure 2F . The adsorption peak of LNT at 3369.3 cm –1 was assigned to the –OH stretching vibrations ( 25 ), which was shifted to 3325.1 cm –1 for LNT-SeNPs, suggesting the strong bonding interaction between hydroxyl groups of LNT and SeNPs. No obvious shifts of other absorption peaks were observed.…”

Section: Resultsmentioning

confidence: 99%

Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

et al. 2022

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Selenium nanoparticles (SeNPs) have gained extensive attention for their excellent biological activity and low toxicity. However, SeNPs are extremely liable to aggregate into non-bioactive or gray elemental selenium, which limits their application in the biomedicine field. This study aimed to prepare stable SeNPs by using lentinan (LNT) as a template and evaluate its anti-colon cancer activity. The average particle diameter of obtained lentinan-selenium nanoparticles (LNT-SeNPs) was approximately 59 nm and presented zero-valent, amorphous, and spherical structures. The monodisperse SeNPs were stabilized by LNT through hydrogen bonding interactions. LNT-SeNPs solution remained highly stable at 4°C for at least 8 weeks. The stability of LNT-SeNPs solution sharply decreased under high temperature and strong acidic conditions. LNT-SeNPs showed no obvious cytotoxic effect on normal cells (IEC-6) but significantly inhibited the proliferation of five colon cancer cells (HCT-116, HT-29, Caco-2, SW620, and CT26). Among them, LNT-SeNPs exhibited the highest sensitivity toward HCT-116 cells with an IC50 value of 7.65 μM. Also, LNT-SeNPs displayed better cancer cell selectivity than sodium selenite and selenomethionine. Moreover, LNT-SeNPs promoted apoptosis of HCT-116 cells through activating mitochondria-mediated apoptotic pathway. Meanwhile, LNT-SeNPs induced cell cycle arrest at G0/G1 phase in HCT-116 cells via modulation of cell cycle regulatory proteins. The results of this study indicated that LNT-SeNPs possessed strong potential application in the treatment of colorectal cancer (CRC).

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Section: Resultsmentioning

confidence: 99%

Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

et al. 2022

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“…Previous research has indicated that the biological activity of polysaccharides was dependent on their chemical composition [ 7 , 26 , 27 ]. We analyzed the total saccharide, protein, and polyphenol contents of LBPs with various molecular weights.…”

Section: Resultsmentioning

confidence: 99%

“…Using the phenol-sulfuric acid method with glucose as the standard, the total saccharide content was calculated [ 26 ]. The protein concentration was measured by a BCA protein assay kit (P0012S, Beyotime Biotechnology Corp., Shanghai, China), using BSA as the standard [ 74 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides

et al. 2023

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The molecular weight, the triple-helix conformation, the monosaccharide content, the manner of glycosidic linkages, and the polysaccharide conjugates of polysaccharides all affect bioactivity. The purpose of this study was to determine how different molecular weights affected the bioactivity of the Lycium barbarum polysaccharides (LBPs). By ethanol-graded precipitation and ultrafiltration membrane separation, one oligosaccharide (LBPs-1, 1.912 kDa) and two polysaccharides (LBPs-2, 7.481 kDa; LBPs-3, 46.239 kDa) were obtained from Lycium barbarum. While the major component of LBPs-1 and LBPs-2 was glucose, the main constituents of LBPs-3 were arabinose, galactose, and glucose. LBPs-2 and LBPs-3 exhibited triple-helix conformations, as evidenced by the Congo red experiment and AFM data. Sugar residues of LBPs-2 and LBPs-3 were elucidated by NMR spectra. The polysaccharides (LBPs-2 and LBPs-3) exhibited much higher antioxidant capacities than oligosaccharide (LBPs-1). LBPs-3 showed higher oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD) activity than LBPs-2, but a lower capability for scavenging ABTS+ radicals. In zebrafish, LBPs-2 and LBPs-3 boosted the growth of T-lymphocytes and macrophages, enhanced the immunological response, and mitigated the immune damage generated by VTI. In addition to the molecular weight, the results indicated that the biological activities would be the consequence of various aspects, such as the monosaccharide composition ratio, the chemical composition, and the chemical reaction mechanism.

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“…Peony is widely planted in China, and peony seeds contain high omega-3 fatty acids (Qu et al, 2017;Wang et al, 2022). The polysaccharides extracted from peony seed dreg displayed high anti-oxidant (Shi et al, 2016a;Shi et al, 2016b;Zhang et al, 2022), and anti-cancerous properties (Zhang et al, 2017). After chemical modifications, the antioxidant activities (Li et al, 2018b;Li et al, 2021) and antibacterial activities (Li et al, 2018a;Liu et al, 2022) were increased significantly.…”

Section: Introductionmentioning

confidence: 99%

Insights into sub-chronic toxicity effects of enzymatic hydrolysate of peony seed meal derived Maillard reaction products in SD rats

et al. 2023

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Despite the use of Maillard reaction products (MRPs) as food flavoring and coloring agents, they are also known to constitute acrylamide, heterocyclic amines, advanced glycosylation end products, and precursors of low molecular weight compounds. The very little is known about the toxicology of peony seed meal-derived MRPs. In this study, sub-chronic toxicity of peony seed meal derived MRPs was evaluated in Sprage-Dawley (SD) rats. Results showed that the body weight, hematology and serum biochemistry, organ coefficient and histopathology of SD rats with daily intake of peony seed meal-derived MRPs lower than 0.45 g/kg were not affected. After feeding with peony seed meal-derived MRPs for 13-weeks, there were no significant toxic effect on either male or female rats. This study provides a theoretical basis for the safety of MRPs derived from the reactants of enzymatic hydrolysis of peony seed meal.

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Physicochemical and antioxidant properties of Lycium barbarum seed dreg polysaccharides prepared by continuous extraction

Cited by 26 publications

References 39 publications

Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides

Insights into sub-chronic toxicity effects of enzymatic hydrolysate of peony seed meal derived Maillard reaction products in SD rats

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