Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

Guan, Xiong; Yao, Yanting; Chen, Xujie; Lin, Xiaorong; Yang, Xiaobing; Ho, Chi‐Tang; Li, Bin; Chen, Zhongzheng

doi:10.3389/fnut.2022.987807

Cited by 9 publications

(3 citation statements)

References 43 publications

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“…These findings support the hypothesis that cells are driven out of the cell cycle under the combined effect of Sor-PDNP and PTT. Our results are in line with other outcomes found in the literature, such as the study conducted by Rahman et al on zinc oxide nanoparticles for CRC treatments or the recently published study by Gao et al on lentinan-functionalized selenium nanoparticles . It is crucial to emphasize that Sor-PDNPs, aside from yielding similar outcomes, are entirely organic: this characteristic significantly reduces concerns related to biodegradability or extended retention of the material within the body.…”

Section: Discussionsupporting

confidence: 91%

Drug-Loaded Polydopamine Nanoparticles for Chemo/Photothermal Therapy against Colorectal Cancer Cells

Carmignani,

Battaglini,

Marino

et al. 2024

ACS Appl. Bio Mater.

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Colorectal cancer (CRC) is a common and deadly malignancy, ranking second in terms of mortality and third in terms of incidence on a global scale. The survival rates for CRC patients are unsatisfactory primarily because of the absence of highly effective clinical strategies. The efficacy of existing CRC treatments, such as chemotherapy (CT), is constrained by issues such as drug resistance and damage to healthy tissues. Alternative approaches such as photothermal therapy (PTT), while offering advantages over traditional therapies, suffer instead from a low efficiency in killing tumor cells when used alone. In this context, nanostructures can efficiently contribute to a selective and targeted treatment. Here, we combined CT and PTT by developing a nanoplatform based on polydopamine nanoparticles (PDNPs), selected for their biocompatibility, drug-carrying capabilities, and ability to produce heat upon exposure to near-infrared (NIR) irradiation. As a chemotherapy drug, sorafenib has been selected, a multikinase inhibitor already approved for clinical use. By encapsulating sorafenib in polydopamine nanoparticles (Sor-PDNPs), we were able to successfully improve the drug stability in physiological media and the consequent uptake by CRC cells, thereby increasing its therapeutic effects. Upon NIR stimulus, Sor-PDNPs can induce a temperature increment of about 10 °C, encompassing both PTT and triggering a localized and massive drug release. Sor-PDNPs were tested on healthy colon cells, showing minimal adverse outcomes; conversely, they demonstrated excellent efficacy against CRC cells, with a strong capability to hinder cancer cell proliferation and induce apoptosis. Obtained findings pave the way to new synergistic chemo-photothermal approaches, maximizing the therapeutic outcomes against CRC while minimizing side effects on healthy cells.

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Section: Discussionsupporting

confidence: 91%

Drug-Loaded Polydopamine Nanoparticles for Chemo/Photothermal Therapy against Colorectal Cancer Cells

Carmignani,

Battaglini,

Marino

et al. 2024

ACS Appl. Bio Mater.

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“…A variety of oligosaccharides, including chitosan [ 44 , 45 ], dextran [ 46 ], mushroom polysaccharides [ 3 , 6 ], and protein [ 4 ] have been developed for coating SeNPs. Among them, LNT is one of the most attempting coating materials with a clearly demonstrated effectiveness for treating various types of tumor cells or xenografts, such as melanoma [ 47 ], hepatocellular carcinoma [ 7 ], malignant ascites and ovarian adenocarcinoma [ 11 ], lung cancer [ 48 ], and colon carcinoma [ 49 ], in preclinical studies. However the conventional approach of constructing image-guided or theranostic SeNPs requires chemical conjugation of imaging probes to the surface of nanoparticles, which will increase the complexity of preparation procedures and impose additional components to the system, thus unfortunately hampering future clinical translation substantially.…”

Section: Discussionmentioning

confidence: 99%

Theranostic Cancer Treatment Using Lentinan-Coated Selenium Nanoparticles and Label-Free CEST MRI

Liu

Ling

et al. 2022

Pharmaceutics

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Selenium nanoparticle (SeNP)-based nanotherapeutics have become an emerging cancer therapy, while effective drug delivery remains a technical hurdle. A theranostic approach, through which imaging companions are integrated with SeNPs, will allow image-guided drug delivery and, therefore, is highly desirable. Traditional methods require the chemical conjugation of imaging agents to the surface of nanoparticles, which may impede the later clinical translation. In this study, we developed a label-free strategy in which lentinan-functionalized SeNPs (LNT-SeNPs) are detected using MRI by the hydroxyl protons carried on LNT molecules. The in vitro phantom study showed that LNT and LNT-SeNPs have a strong CEST signal at 1.0 ppm apart from the water resonance, suggesting an in vivo detectability in the µM concentration range. Demonstrated on CT26 colon tumor cells, LNT-SeNPs exert a strong anticancer effect (IC50 = 4.8 μM), prominently attributed to the ability to generate intracellular reactive oxygen species. However, when testing in a mouse model of CT26 tumors, administration of LNT-SeNPs alone was found unable to deliver sufficient drugs to the tumor, leading to poor treatment responses. To improve the drug delivery, we co-injected LNT-SeNPs and TNF-α, a previously reported drug that could effectively damage the endothelial cells in the tumor vasculature, thereby increasing drug delivery to the tumor. Our results revealed a 75% increase in the intratumoral CEST MRI signal, indicating a markedly increased delivery efficiency of LNT-SeNPs when combined with TNF-α. The combination therapy also resulted in a significantly enhanced treatment outcome, as revealed by the tumor growth study. Taken together, our study demonstrates the first label-free, SeNP-based theranostic system, in which LNT was used for both functional surface coating and CEST MRI signal generating. Such a theranostic LNT-SeNP system is advantageous because it requires chemical labeling and, therefore, has high biocompatibility and low translatable barriers.

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“…Lentinan is used in the treatment of various malignancies, including liver cancer [ 69 ], gastric cancer [ 70 ], lung cancer [ 71 ], colorectal cancer [ 72 ], ovarian cancer [ 73 ], pancreatic cancer [ 74 ], and others. It plays a significant role in enhancing the effectiveness of chemotherapy and radiotherapy, as well as improving the quality of life during tumor treatment.…”

Section: Clinical Application Of Polysaccharide Preparation In the Tr...mentioning

confidence: 99%

Review on active components and mechanism of natural product polysaccharides against gastric carcinoma

Liu,

Sun,

Jin

et al. 2024

Heliyon

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Lentinan-functionalized selenium nanoparticles induce apoptosis and cell cycle arrest in human colon carcinoma HCT-116 cells

Cited by 9 publications

References 43 publications

Drug-Loaded Polydopamine Nanoparticles for Chemo/Photothermal Therapy against Colorectal Cancer Cells

Drug-Loaded Polydopamine Nanoparticles for Chemo/Photothermal Therapy against Colorectal Cancer Cells

Theranostic Cancer Treatment Using Lentinan-Coated Selenium Nanoparticles and Label-Free CEST MRI

Review on active components and mechanism of natural product polysaccharides against gastric carcinoma

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