Purification, cDNA structure and biological significance of a single insulin-like growth factor-binding domain protein (SIBD-1) identified in the hemocytes of the spider Cupiennius salei

Insulin-like growth factor binding protein 7, a member of insulin-like growth factor signal pathway, involved in immune response of small abalone Haliotis diversicolor

“…Analysis of conserved domains revealed the presence of an IGFBP N-terminal domain (IB) between amino acids 22 and 88, a kazal-type serine proteinase inhibitor domain (KI) between amino acids 89 and 128, and an immunoglobulin-like C2 domain (IgC2) between amino acids 144 and 223, is consistent with human IGFBP7, spider CsaIGFBP7 and tick AamIGFBP7 sequence features [8,22,32]. Additionally, two IGFBP7 characteristic motifs were identified from the putative amino acid sequence of saIGFBP7: IB domain signature (xCGCCxxC) and KI domain signature (CGxDxxTYxN) (Fig.…”

“…These proteins, which are likely to be a member of the IGFBP superfamily, are participated in the shrimp and crab immune responses by bacterial challenge [20,21]. In addition, a decrease of the temporal SIBD-1 expression was detected in hemocytes after a mixture of Escherichia coli and Staphylococcus aureus was injected into spider Cupiennius salei [22]. These SIBDs cited above have presented high similarities with the N-terminal conserved IB domain of IGFBPs and then were led to the proposal of the invertebrate counterparts of IGFBPs [20].…”

Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

“…The Sv-SIBD2 and 4 branch alongside the crustacean hematopoietic factor (CHF) of Pacifastacus leniusculus, expressed in hematopoietic tissue and responsible for preventing hemocyte apoptosis (Lin et al, 2011). Neighbouring SIBDs were isolated from shrimp (L. vannamei) hemocytes, (Castellanos et al, 2008) and spider (C. salei) hemocytes, nerve mass and muscle (Kuhn-Nentwig et al, 2011) which were up and down-regulated, respectively, in response to bacterial challenge; indication of an immune response (Castellanos et al, 2008;Kuhn-Nentwig et al, 2011;Trachsel et al, 2012). The more distant SIBD of the crab E. sinensis, was found to be broadly expressed and was once again up-regulated under immune stress (Gai et al, 2010).…”

An economic dilemma between weapon systems may explain an arachno-atypical venom in wasp spiders (Argiope bruennichi)

“…This class of protein has not been detected in other spider venoms. The A. bruennichi IGFBP is closely related to a protein found in the venom of the tiger wandering spider Cupiennius salei (Kuhn-Nentwig et al, 2011). Such proteins are commonly found in arachnid venoms but also in scorpions (Superstitia donensis, Hadrurus spadix and Centruroides hentzi) and ticks of the genus Amblyomma (Santibanez-Lopez et al, 2016;Esteves et al, 2017;Rokyta and Ward, 2017;Ward et al, 2017).…”