Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

Chandler, Jennifer C.; Aizen, Joseph; Elizur, Abigail; Hollander-Cohen, Lian; Battaglene, SC; Ventura, Tomer

doi:10.1016/j.ygcen.2014.08.018

Cited by 61 publications

(64 citation statements)

References 52 publications

(102 reference statements)

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“…The lengthy transition, the large-sized larvae and their transparency enable clear molt staging by gut retraction. The availability of transcriptomic data for both the larval metamorphic transition at high resolution [35], alongside tissues of juveniles and adults from this species [44][45][46][47][48][49] enables thorough examination of expression patterns and correlation with spatial-temporal expression.…”

Section: Metamorphosis In Spiny Lobstersmentioning

confidence: 99%

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Ventura

Bose

Fitzgibbon

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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, CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development, Journal of Steroid Biochemistry and Molecular Biologyhttp://dx.doi.org/10. 1016/j.jsbmb.2017.04.007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

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Section: Metamorphosis In Spiny Lobstersmentioning

confidence: 99%

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Ventura

Bose

Fitzgibbon

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

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“…Lobsters were reared as previously described in Fitzgibbon and Battaglene [2012], under which conditions the genital openings (gonopores) appear post-metamorphosis in the juvenile at ∼ 1 g ( Sv-IAG can first be identified at that stage), testis development starts to become evident between 200-500 g, and an individual is considered to be fully mature at ∼ 2 kg. Total RNA was isolated from the testis, ovary [Chandler et al, 2015], male and female brain, eyestalk and antennal gland of 2 mature S. verreauxi males (M1 weighing 3.11 kg and M2 weighing 3.34 kg) and 2 mature females (intermolt, 2.76 and 3.11 kg). Androgenic glands (AG1 and AG2) were taken from M1 and M2 respectively; the gland from M1 (AG1) was hypertrophied via eyestalk-ablation 14 days before cull in order to remove the central neuroendocrine system, preventing its regulatory effects on the AG [Khalaila et al, 2002;Webster et al, 2012].…”

Section: Sample Preparation and Sequencingmentioning

confidence: 99%

“…All culls were undertaken in April 2013. For brain and eyestalk samples [Chandler et al, 2015;Ventura et al, 2014b], tissue from 2 additional juveniles of each sex (each weighing 5 g), was also included to increase RNA extraction quantity. RNA extracted from the muscular region of the fifth walking-leg was taken from male and female juveniles (each weighing 5 g).…”

Section: Sample Preparation and Sequencingmentioning

confidence: 99%

“…RNA was extracted from those tissues described above, as well as from male and female hepatopancreas and male muscle (to add further description to the spatial expression profile than was provided through sequence data). The cDNA was prepared and the RT-PCR conducted as described in Chandler et al [2015]. 16S was used as a housekeeping gene (positive control) to ensure the quality of each cDNA sample.…”

Section: Rt-pcr Validationmentioning

confidence: 99%

“…These proteins are characterized as large plasma proteins that chaperone proteins such as insulin and insulin-like growth factors, preventing their proteolysis when in circulation [Borth, 1992]. Considering that orthologues of human insulin-binding proteins have been identified in S. verreauxi , Sv-IGFBP [Chandler et al, 2015] with proof that the IGFBP is involved in IAG binding [Rosen et al, 2013b], it may be that these AGenriched, plasma proteins function to the same end, regulating the transportation and bioavailability of IAG.…”

Section: Functional Characterization Of the Mature Androgenic Gland Dmentioning

confidence: 99%

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Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Chandler

Aizen²,

Elizur³

et al. 2015

Sex Dev

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The Eastern spiny lobster, Sagmariasus verreauxi, is commercially important in fisheries, with growing aquaculture potential, driving an interest to better understand male sexual differentiation. Amongst the Decapoda, the androgenic gland (AG) and the insulin-like androgenic gland hormone (IAG) have a well-defined function in male sexual differentiation. However, IAG is not a sex determinant and therefore must be considered as part of a broader, integrated pathway. This work uses a transcriptomic, multi-tissue approach to provide an integrated description of male-biased expression as mediated through the AG. Transcriptomic analyses demonstrate that IAG expression is stage- and eyestalk-regulated (low in immature, high in mature and 6-times higher in hypertrophied glands), with IAG being the predominant AG-specific factor. The low expression of this key factor in immature males suggests the involvement of other tissues in male sexual differentiation. Across tissues, the gonad (87.8%) and antennal gland (73.5%) show the highest male-biased differential expression of transcripts and also express 4 sex-determination regulators, known as Dmrts, with broader expression of Sv-Sxl and Sv-TRA-2. In order to better understand male sexual differentiation, tissues other than the AG must also be considered. This research highlights the gonad and antennal gland as showing significant male-biased expression patterns, including the Sv-Dmrts.

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Monosex in Aquaculture

Ventura

2018

Results and Problems in Cell Differentiation

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Monosex refers to the culture of either all-male or all-female populations, a sought after approach in aquaculture. This chapter reviews the advantages of monosex population culture and details the mechanisms to achieve it based on different modes of sex determination and sexual differentiation. A recent case study for an aquaculture biotechnology based on sexual manipulation in crustaceans serves in this chapter to identify the key elements for a successful application. This application which makes use of RNA interference with a key regulating hormone opens the pathway toward environmentally friendly applications in fish and additional aquacultured species. This chapter portrays the state of the art in sexual manipulations in aquacultured species, starting with vertebrate species, followed by the case study of the crustacean species and discussion on how the techniques used in this study are applicable for other species.

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Discovery of a novel insulin-like peptide and insulin binding proteins in the Eastern rock lobster Sagmariasus verreauxi

Cited by 61 publications

References 52 publications

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Monosex in Aquaculture

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