CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Ventura, Tomer; Bose, Utpal; Fitzgibbon, Quinn P.; Smith, Gregory G.; Shaw, P. Nicholas; Cummins, Scott F.; Elizur, Abigail

doi:10.1016/j.jsbmb.2017.04.007

Cited by 22 publications

(23 citation statements)

References 43 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2), making it the leading candidate. However, conclusive identification of the MIH receptor awaits a functional assay that shows MIH activation of MIH receptor candidates expressed in a heterologous reporting system [15, 153]. The identification of FMRFamide, serotonin, and octopamine receptors raise the possibility that the YO is controlled by direct innervation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle

et al. 2019

Self Cite

View full text Add to dashboard Cite

BackgroundG-protein coupled receptors (GPCRs) are ancient, ubiquitous, constitute the largest family of transducing cell surface proteins, and are integral to cell communication via an array of ligands/neuropeptides. Molt inhibiting hormone (MIH) is a key neuropeptide that controls growth and reproduction in crustaceans by regulating the molt cycle. It inhibits ecdysone biosynthesis by a pair of endocrine glands (Y-organs; YOs) through binding a yet uncharacterized GPCR, which triggers a signalling cascade, leading to inhibition of the ecdysis sequence. When MIH release stops, ecdysone is synthesized and released to the hemolymph. A peak in ecdysone titer is followed by a molting event. A transcriptome of the blackback land crab Gecarcinus lateralis YOs across molt was utilized in this study to curate the list of GPCRs and their expression in order to better assess which GPCRs are involved in the molt process.ResultsNinety-nine G. lateralis putative GPCRs were obtained by screening the YO transcriptome against the Pfam database. Phylogenetic analysis classified 49 as class A (Rhodopsin-like receptor), 35 as class B (Secretin receptor), and 9 as class C (metabotropic glutamate). Further phylogenetic analysis of class A GPCRs identified neuropeptide GPCRs, including those for Allatostatin A, Allatostatin B, Bursicon, CCHamide, FMRFamide, Proctolin, Corazonin, Relaxin, and the biogenic amine Serotonin. Three GPCRs clustered with recently identified putative CHH receptors (CHHRs), and differential expression over the molt cycle suggests that they are associated with ecdysteroidogenesis regulation. Two putative Corazonin receptors showed much higher expression in the YOs compared with all other GPCRs, suggesting an important role in molt regulation.ConclusionsMolting requires an orchestrated regulation of YO ecdysteroid synthesis by multiple neuropeptides. In this study, we curated a comprehensive list of GPCRs expressed in the YO and followed their expression across the molt cycle. Three putative CHH receptors were identified and could include an MIH receptor whose activation negatively regulates molting. Orthologs of receptors that were found to be involved in molt regulation in insects were also identified, including LGR3 and Corazonin receptor, the latter of which was expressed at much higher level than all other receptors, suggesting a key role in YO regulation.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5363-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent advances in omics approaches enable the depiction of the expression pattern of many genes and to estimate the regulatory interactions involved in metamorphosis in the prawn ( M. rosenbergii ) ( 33 ), the shrimp ( Neocaridina denticulata ) ( 34 ), and the spiny lobster ( Sagmariasus verreauxi ) ( 35 , 36 ). Although the aforementioned transcriptome studies provided various new insights, the number of unannotated genes has hampered the completion of more comprehensive analysis due to the lack of publicly available genomes.…”

Section: Discussionmentioning

confidence: 99%

Impacts of Methyl Farnesoate and 20-Hydroxyecdysone on Larval Mortality and Metamorphosis in the Kuruma Prawn Marsupenaeus japonicus

2020

View full text Add to dashboard Cite

Physiological functions of juvenile hormone (JH) and molting hormone have been demonstrated in insects. JH, molting hormone and their mimics (insect growth regulators, IGRs) show endocrine-disrupting effects not only on target pest insects but also on other arthropod species such as crustaceans. However, little is known about the endocrine-disrupting effects of IGRs on benthic crustaceans. In this study, laboratory experiments were conducted to investigate effects of representative innate JH in crustaceans (methyl farnesoate, MF) and molting hormone (20-hydroxyecdysone, 20E, active form of ecdysteroid) on larval stages of the kuruma prawn Marsupenaeus japonicus, which is a decapod crustacean living in warm seawater. Larval development of kuruma prawn progresses in the order of nauplius, zoea, mysis, and then post-larvae with molting and metamorphosis, but it is unknown whether both MF and 20E have crucial roles in metamorphosis and molting of this species. Treatments of either MF or 20E on shrimp larvae were attempted at each developmental stage and those effects were validated. In terms of EC 50 values between mortality and metamorphosis, there were apparent differences in the transition from nauplius to zoea (MF: 7.67 and 0.12 µM; 20E: 3.84 and 0.06 µM in survival and metamorphic rates, respectively). In contrast, EC 50 values in MF and 20E treatments showed high consistency in the transitions between zoea to mysis (EC 50 values for survival; MF: 1.25 and 20E: 0.22 µM), and mysis to post-larvae (EC 50 values for survival; MF: 0.65 and 20E: 0.46 µM). These data suggest that nauplius has strong resistance against exposure to MF and 20E. Moreover, both chemicals induced high mortality triggered by the disruption of molting associated with metamorphosis. To our knowledge, this is the first experimental evidence that investigates in vivo physiological functions of MF and 20E in the larval stages of kuruma prawn, shedding light on not only ecotoxicological impacts of IGRs released into nature, but also endocrine mechanisms underlying larval development with metamorphosis in benthic decapod crustaceans.

show abstract

“…In addition, a membrane guanylyl cyclase (GC-II) is considered as the receptor activated by MIH, resulting in immediate increase of the intracellular messenger cGMP level ( Kim et al., 2004 ; Lee et al., 2007 ). Therefore, conclusive identification of the CHH/MIH receptor awaits a functional assay that shows CHH/MIH activation of their putative receptor expressed in a heterologous reporting system ( Aizen et al., 2016 ; Ventura et al., 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Wang

et al. 2021

PeerJ

View full text Add to dashboard Cite

Neuropeptides and their G protein-coupled receptors (GPCRs) regulate multiple physiological processes. Currently, little is known about the identity of native neuropeptides and their receptors in Portunus trituberculatus. This study employed RNA-sequencing and reverse transcription-polymerase chain reaction (RT-PCR) techniques to identify neuropeptides and their receptors that might be involved in regulation of reproductive processes of P. trituberculatus. In the central nervous system transcriptome data, 47 neuropeptide transcripts were identified. In further analyses, the tissue expression profile of 32 putative neuropeptide-encoding transcripts was estimated. Results showed that the 32 transcripts were expressed in the central nervous system and 23 of them were expressed in the ovary. A total of 47 GPCR-encoding transcripts belonging to two classes were identified, including 39 encoding GPCR-A family and eight encoding GPCR-B family. In addition, we assessed the tissue expression profile of 33 GPCRs (27 GPCR-As and six GPCR-Bs) transcripts. These GPCRs were found to be widely expressed in different tissues. Similar to the expression profiles of neuropeptides, 20 of these putative GPCR-encoding transcripts were also detected in the ovary. This is the first study to establish the identify of neuropeptides and their GPCRs in P. trituberculatus, and provide information for further investigations into the effect of neuropeptides on the physiology and behavior of decapod crustaceans.

show abstract

CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Cited by 22 publications

References 43 publications

Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle

Characterization of G-protein coupled receptors from the blackback land crab Gecarcinus lateralis Y organ transcriptome over the molt cycle

Impacts of Methyl Farnesoate and 20-Hydroxyecdysone on Larval Mortality and Metamorphosis in the Kuruma Prawn Marsupenaeus japonicus

Identification and characterization of expression profiles of neuropeptides and their GPCRs in the swimming crab, Portunus trituberculatus

Contact Info

Product

Resources

About