Purification and Characterization of Pepsin-Solubilized Collagen from Skin and Connective Tissue of Giant Red Sea Cucumber (Parastichopus californicus)

Abstract

Pepsin-solubilized collagen (PSC) was extracted from giant red sea cucumbers ( Parastichopus californicus ) and characterized for denaturation temperature (T(d)), maximum transition temperature (T(m)), enzyme-digested peptide maps, and gel-forming capability. SDS-PAGE showed that PSCs from giant red sea cucumber skin and connective tissue were both type I collagens, consisting of three alpha(1) chains of approximately 138 kDa each. The amino acid composition and peptide maps of PSCs digested by V8 protease were different from those of calf skin type I collagen. The T(d) and T(m) are 18.5 and 33.2 degrees C, respectively, for skin PSC and are 17.9 and 32.7 degrees C, respectively, for connective tissue PSC. Both skin and connective tissue PSCs exhibited good gel-forming capability at pH 6.5 and at an ionic strength of 300 mM salt (NaCl). Collagen isolated from giant red sea cucumbers might be used as an alternative to mammalian collagen in the food and pharmaceutical industries.

Isolation, Characterization and Evaluation of Collagen from Jellyfish Rhopilema esculentum Kishinouye for Use in Hemostatic Applications

“…It had been reported that the collagen isolated from marine invertebrate animals, such as Red Sea cucumber ( Stichopus japonicus ) [55], sea urchin ( Asthenosoma ijima ) [56], and starfish ( Asterias amurensis ) [57], was classified into type I collagen. Moreover, the marine vertebrate collagen, such as scales and skin of fish also belongs to type I collagen [58–60]. SDS-PAGE profiles of ASC and PSC from jellyfish showed a similar pattern, demonstrated that the ASC and PSC extracted from jellyfish mesoglea were similar to type I collagen.…”

Amino acid composition and functional properties of giant red sea cucumber (Parastichopus californicus) collagen hydrolysates

“…Collagen in the skin of giant red sea cucumbers was extracted as previously described (Liu et al, 2010). The proximate compositions of moisture, lipid, ash and protein contents of the collagen were determined by the AOAC methods (AOAC, 1995).…”

Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)

“…Several collagens including Type IeXXVIII from animal origin have been reported. Different types possess varying molecular properties (Liu, Oliveira, & Su, 2010;Shoulders & Raines, 2009). Amongst all collagens, type I collagen has been found as the most common in fish and mammal.…”