Isolation, Characterization and Evaluation of Collagen from Jellyfish Rhopilema esculentum Kishinouye for Use in Hemostatic Applications

Cheng, Xigao; Shao, Ziyu; Li, Chengbo; Yu, Lejun; Raja, Mazhar Ali; Liu, Chenguang

doi:10.1371/journal.pone.0169731

Cited by 122 publications

(102 citation statements)

References 82 publications

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“…The absorbance of the resulting hemoglobin solution was measured at 540 nm by using a Lambda 25 UV‐visible spectrophotometer (Perkin Elmer). The blood clotting index (BCI) was calculated on the basis of Equation

BCI = \frac{D_{normals}}{D_{0}},

where D s is the absorbance of the resulting hemoglobin solution mentioned above, and D 0 is the absorbance of 100 μL of whole blood hemolyzed with 2.5 mL of deionized water.…”

Section: Methodsmentioning

confidence: 99%

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Xing

et al. 2019

J Biomed Mater Res

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Developing a small‐diameter vascular graft with a satisfactory performance in terms of mechanical and biological properties remains a challenging issue because of comprehensive requirements from clinical applications. Polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels exhibit many desirable characteristics for small‐diameter vascular grafts because of their tunable mechanical properties, especially high compliance. However, poor cells adhesion hinders their application for endothelialization in situ. Therefore, in the present work, polydopamine (PDA) and tetrapeptide Arg‐Glu‐Asp‐Val (REDV) were used to functionalize the hydrogels surface and improve cells adhesion. A series of characterizations were systematically conducted to examine the applicability of coated hydrogels to small‐diameter vascular grafts. Results showed that bare and coated hydrogels have appropriate structural stability, and no significant differences in tensile properties could be found after being coated with PDA or PDA‐REDV. The hydrophilicity of the hydrogels decreased with the coatings of PDA and especially PDA‐REDV to improve protein adsorption, porcine iliac artery endothelial cells (PIECs) adhesion, viability, proliferation, and spreading on the hydrogels. Lower hemolysis percentages and higher blood clotting index values were attained for the hydrogels, suggesting their satisfactory hemocompatibility. Overall, the present work provided insights into the development of a novel hydrogel‐based small‐diameter vascular graft. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:117–127, 2020.

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BCI = \frac{D_{normals}}{D_{0}},

where D s is the absorbance of the resulting hemoglobin solution mentioned above, and D 0 is the absorbance of 100 μL of whole blood hemolyzed with 2.5 mL of deionized water.…”

Section: Methodsmentioning

confidence: 99%

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Xing

et al. 2019

J Biomed Mater Res

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“…Recently, the jellyfish R. esculentum has received some attention as the type I collagen extracted from this species is quite similar to the human type, making it suitable for different applications in the biomedical field [10]. It was cross-linked with 1-Ethyl 3-(3-Dimethylaminoprophyl)-Carbodiimide (EDC) to form collagen-based sponges which showed hemostatic properties as blood clotting after tail amputation in rats, suggesting that it may be a suitable candidate for hemostatic material and wound-dressing applications [31]. Moreover, peptides derived from R. esculentum collagen were involved in wound-healing processes in vivo, by increasing the production of chemotactic factors TGF-β1 and β-FGF [124].…”

Section: Jellyfishmentioning

confidence: 99%

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

et al. 2020

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Due to its unique properties, collagen is used in the growing fields of pharmaceutical and biomedical devices, as well as in the fields of nutraceuticals, cosmeceuticals, food and beverages. Collagen also represents a valid resource for bioplastics and biomaterials, to be used in the emerging health sectors. Recently, marine organisms have been considered as promising sources of collagen, because they do not harbor transmissible disease. In particular, fish biomass as well as by-catch organisms, such as undersized fish, jellyfish, sharks, starfish, and sponges, possess a very high collagen content. The use of discarded and underused biomass could contribute to the development of a sustainable process for collagen extraction, with a significantly reduced environmental impact. This addresses the European zero-waste strategy, which supports all three generally accepted goals of sustainability: sustainable economic well-being, environmental protection, and social well-being. A zero-waste strategy would use far fewer new raw materials and send no waste materials to landfills. In this review, we present an overview of the studies carried out on collagen obtained from by-catch organisms and fish wastes. Additionally, we discuss novel technologies based on thermoplastic processes that could be applied, likewise, as marine collagen treatment.2 of 23 protective colloid functions and film-forming capacity. Also, collagen is a good surface-active agent, with its ability to penetrate lipid-free interfaces [2].Collagen can be utilized in a variety of applications because of its biocompatibility and excellent degradability [3,4]. Furthermore, it is known that collagen is a molecule with weak immunogenicity, which decreases the possibilities of rejection when it is ingested or injected into a different body. Although this molecule has already low antigenicity, this property can be enhanced by modifying it to suppress any immune response [5,6]. Additionally, collagen peptides and gelatin (denatured collagen) have been widely utilized in different fields such as food, medicine, cosmetics, leather and film industries, diagnostic imaging, and therapeutic delivery [7].For many years, most available collagen was extracted from discards from the bovine and porcine processing industries, but during the last few decades the use of collagen from these sources has been limited. The use of porcine and bovine-derived products is occasionally prevented by dietary regimes, due to specific needs or personal choices. It is forbidden by religious constraints, to Muslims, Hindus and Jews who make up 38.4% of the global population [8]. Moreover, the use of bovine-derived products became a concern for a wider section of the population during the bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE) and foot-and-mouth disease (FMD) crises that occurred over the last few decades in all areas of the world, mostly in the United Kingdom and Asia. Bovine-derived products might be a vehicle of transmission of these diseases ...

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“…Other sources such as marine collagen are arising as a relevant alternative to their mammalian counterparts. Several organisms such as marine sponges [5,6], jellyfish [7,8], squid [9,10], and fishes [11][12][13][14] have been exploited for the extraction of marine collagen. About 75% of a fish's…”

Section: Introductionmentioning

confidence: 99%

Cosmetic Potential of Marine Fish Skin Collagen

et al. 2017

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Many cosmetic formulations have collagen as a major component because of its significant benefits as a natural humectant and moisturizer. This industry is constantly looking for innovative, sustainable, and truly efficacious products, so marine collagen based formulations are arising as promising alternatives. A solid description and characterization of this protein is fundamental to guarantee the highest quality of each batch. In the present study, we present an extensive characterization of marine-derived collagen extracted from salmon and codfish skins, targeting its inclusion as component in cosmetic formulations. Chemical and physical characterizations were performed using several techniques such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier Transformation Infrared (FTIR) spectroscopy rheology, circular dichroism, X-ray diffraction, humidity uptake, and a biological assessment of the extracts regarding their irritant potential. The results showed an isolation of type I collagen with high purity but with some structural and chemical differences between sources. Collagen demonstrated a good capacity to retain water, thus being suitable for dermal applications as a moisturizer. A topical exposure of collagen in a human reconstructed dermis, as well as the analysis of molecular markers for irritation and inflammation, exhibited no irritant potential. Thus, the isolation of collagen from fish skins for inclusion in dermocosmetic applications may constitute a sustainable and low-cost platform for the biotechnological valorization of fish by-products.

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Isolation, Characterization and Evaluation of Collagen from Jellyfish Rhopilema esculentum Kishinouye for Use in Hemostatic Applications

Cited by 122 publications

References 82 publications

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

Cosmetic Potential of Marine Fish Skin Collagen

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