Protein Binding Capacity of Different Forages Tannin

Yusiati, Lies Mira; Kurniawati, Asih; Hanim, Chusnul; Anas, Muhsin Al

doi:10.1088/1755-1315/119/1/012007

Cited by 13 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is because the complex bonds of condensed tannins and proteins will decompose at an acidic pH, and proteins can be digested in the abomasum. Yusiati et al (2018) stated that condensed tannins could increase protein that escapes rumen degradation due to its ability to bind protein at neutral pH conditions. Still, at acidic pH conditions such as in the abomasum, protein will be released to digested in the abomasum and intestine.…”

Section: Resultsmentioning

confidence: 99%

“…Mahogany leaves contain 10,03 mg/100 mg DM condensed tannins, and the ability of mahogany to bind protein reaches 1.217 mg BSA/mg DM (Yusiati et al 2018). Crude protein of soybean meal reached 38,90% or equivalent to 389 mg of CF, so it was found that every 1 mg of condensed tannins in mahogany leaves could bind 336.89 mg of protein or every 1gram DM of soybean meal could be bound with condensed tannins of 3%.…”

Section: Methodsmentioning

confidence: 98%

“…The process of protein digestion depends on the separation of the tannin-protein complex in the abomasum because the bonds of the tannin-protein complex cannot wholly release each other, it is related to the different tannins and protein structures (Díaz Carrasco et al 2017). Yusiati et al, (2018) found that mahogany leaves had a total tannin content of 11.9 mg/100 mg DM, consisting of condensed tannins 9.241±0.02mg/100mg DM and hydrolyzed tannins of 2.707±0.06 mg/100 mg DM. The ability of the tannin to bind protein reaches 1,217 mg BSA/mg leaf DM, so that mahogany leaves have the potential to be used as a source of condensed tannins in protein protection for ruminant feed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products

Ifani

Rimbawanto

Suhartati

2021

JITV

View full text Add to dashboard Cite

The study was aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro based on a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (T0); 1.5% (T1); 3% (T2); and 4.5% (T3). Data obtained were analyzed by analysis of variance and tested using orthogonal polynomials. Results showed that addition of protected soybean meal with mahogany leaf extract had a cubical effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effecton N-NH3, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products

Ifani

Rimbawanto

Suhartati

2021

JITV

View full text Add to dashboard Cite

show abstract

“…In the in vitro studies, as proposed in this work, it is described that the substances that cause precipitation of proteins on the surface of the epithelium promote healing, as observed in the case of tannins [21][22][23][24][25][26] . Therefore, since the test was performed using fixed histological sections, it was not possible to test the stimulation of these substances by the tincture; however, the precipitation capacity (proteolysis) of collagen and elastic fibers was studied, which is directly related to the effectiveness of the healing process.…”

Section: Discussionmentioning

confidence: 99%

Employing optical microscopy technique to determine the tissue action of Hamamelis virginianaL. (Hamamelidaceae), Maytenus ilicifoliaMart. ex Reissek (Celastraceae) and Casearia sylvestris Sw. (Salicaceae) tinctures on skin-fixed histological sections of Wistar rats

Dezena

Ireno

Silva

2021

Rev. Ciênc. Farm. Básica Apl.

View full text Add to dashboard Cite

Objective: In this study, we aimed to determine the action of the tinctures of Hamamelis virginiana, Maytenus ilicifolia, and Casearia sylvestris on tissues. For this, we investigated the histological sections of fixed skin tissue of Wistar rats for the changes in collagen and elastic fibers, epithelial cells, conjunctive cells, epidermal attachments, pigments, and granules using the optical microscopy technique. Since in the literature and published articles, the use of in vivo models, such as Wistar rats, is predominant to evaluate the healing action of herbal medicines. Methods: The tinctures of Hamamelis virginiana, Maytenus ilicifolia, and Casearia sylvestris, and ethanol 70% (blank) were used. The tinctures were obtained at 10% (w/v) through percolation using 70% ethanol (v/v) as the extraction liquid. This study was conducted in duplicate for each tincture and different incubation times (4, 24, and 48 h) at 37 °C in an oven. The slides used in this study (Wistar rat skin) were previously processed at the histology laboratory since the waste material was donated to conduct this experiment. The Research Ethics Committee approved the use of animals of the Life Sciences Center of the Pontifical Catholic University of Campinas (PUC-CAMPINAS) under the protocol approval number 367/08. Before initiating the staining process, the slides were removed from the oven, and the tincture deposited on the slides was removed. Weigert staining was performed subsequently. For semi-quantitative analysis, the histological sections were carefully observed, and the number of collagen and elastic fibers was evaluated based on the following scale: (+) normal fiber presence, (−) slight decrease, (− −) moderate decrease, and (− − −) intense decrease. The images were digitally captured to obtain the results using a photomicroscope. Results: The degradation of collagen fibers was best evidenced upon using Hamamelis virginiana tincture, which is concordant with the existing reports in the literature on its healing action via the precipitation of dermal proteins. Maytenus ilicifolia and Casearia sylvestris tinctures exhibited low proteolytic capacity as they only caused degradation of elastic fibers that are more delicate and very different from collagen in their constitution. Therefore, the application of the latter two as healing agents (which acts through precipitating proteins) is limited. Conclusions: This experimental histological model using the optical microscopy technique exhibits the advantage of agility and high efficiency as a simple and powerful approach.

show abstract

“…La concentración de taninos es muy variable, dependiendo de la especie, tipo de tejido, cultivo, estado de desarrollo y condiciones ambientales. El contenido de taninos totales de especies forrajeras, principalmente de la familia Fabaceae, oscilan entre 1,57 a 9,16 g ATE/100 g de muestra seca 17,18 . El contenido de 2 a 5 % de taninos expresado como ácido tánico equivalente se consideran de moderada concentración, a partir de 6 % se considera de alta concentración, en Desmodium molliculum se halló 5,17 ± 0,10 g ATE/100 g de planta seca 19 .…”

Section: Cuantificación De Taninosunclassified

ESTUDIO FITOQUÍMICO CUALITATIVO PRELIMINAR Y CUANTIFICACIÓN DE FLAVONOIDES Y TANINOS DEL EXTRACTO ETANÓLICO DE HOJAS DE Desmodium vargasianum Schubert

Zevallos

2018

rsqp

View full text Add to dashboard Cite

En este trabajo se investigó los principales metabolitos secundarios presentes en el extracto etanólico de hojas de Desmodium vargasianum Schubert “pie de perro”. El tamizaje fitoquímico realizado en el extracto etanólico sugiere la presencia de compuestos fenólicos (flavonoides, taninos) y alcaloides. Se realizó la cromatografía en capa fina, dando positivo flavonoides, taninos y alcaloides. Finalmente se cuantificaron flavonoides totales y taninos totales. En la cuantificación de flavonoides totales por espectrofotometría UV-VIS se obtuvo 202,05 ± 1,10 mg QE/g de extracto etanólico (EE). En cuanto a la cuantificación de taninos totales por espectrofotometría UV-VIS se obtuvo 178,50 ± 0,75 mg ATE/g de EE.

show abstract

Protein Binding Capacity of Different Forages Tannin

Cited by 13 publications

References 8 publications

Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products

Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products

Employing optical microscopy technique to determine the tissue action of Hamamelis virginianaL. (Hamamelidaceae), Maytenus ilicifoliaMart. ex Reissek (Celastraceae) and Casearia sylvestris Sw. (Salicaceae) tinctures on skin-fixed histological sections of Wistar rats

ESTUDIO FITOQUÍMICO CUALITATIVO PRELIMINAR Y CUANTIFICACIÓN DE FLAVONOIDES Y TANINOS DEL EXTRACTO ETANÓLICO DE HOJAS DE Desmodium vargasianum Schubert

Contact Info

Product

Resources

About