Protein adsorption, cell viability and corrosion properties of Ti6Al4V alloy treated by plasma oxidation and anodic oxidation

Bayrak, Özgü; Asl, Hojjat Ghahramanzadeh; Ak, Ayşe

doi:10.1007/s12613-020-2020-5

Cited by 17 publications

(17 citation statements)

References 46 publications

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“…Higher anodizing voltages resulted, on the other hand, in a rutile layer, which is less biocompatible than anatase. On similar surfaces, no enhancement of adsorbed protein was found when high protein concertation solution as FBS was used [ 135 ]. This is a useful reminder that protein adsorption on surfaces is not only dictated by the biomaterials properties but also, and in a significant manner, by the adsorption environment.…”

Section: How the Characteristics Of Titanium Based Biomaterials Inmentioning

confidence: 99%

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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Titanium and its alloys, specially Ti6Al4V, are among the most employed materials in orthopedic and dental implants. Cells response and osseointegration of implant devices are strongly dependent on the body–biomaterial interface zone. This interface is mainly defined by proteins: They adsorb immediately after implantation from blood and biological fluids, forming a layer on implant surfaces. Therefore, it is of utmost importance to understand which features of biomaterials surfaces influence formation of the protein layer and how to guide it. In this paper, relevant literature of the last 15 years about protein adsorption on titanium-based materials is reviewed. How the surface characteristics affect protein adsorption is investigated, aiming to provide an as comprehensive a picture as possible of adsorption mechanisms and type of chemical bonding with the surface, as well as of the characterization techniques effectively applied to model and real implant surfaces. Surface free energy, charge, microroughness, and hydroxylation degree have been found to be the main surface parameters to affect the amount of adsorbed proteins. On the other hand, the conformation of adsorbed proteins is mainly dictated by the protein structure, surface topography at the nano-scale, and exposed functional groups. Protein adsorption on titanium surfaces still needs further clarification, in particular concerning adsorption from complex protein solutions. In addition, characterization techniques to investigate and compare the different aspects of protein adsorption on different surfaces (in terms of roughness and chemistry) shall be developed.

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Section: How the Characteristics Of Titanium Based Biomaterials Inmentioning

confidence: 99%

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

2021

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“…The depletion of Al taking place on HCPN samples may be a beneficial biological effect, once aluminum has been assumed to be detrimental to a wide range of cells, while also being commonly correlated with neurological diseases in patients who received implants, but it may entail loss of mechanical properties to the alloy.…”

Section: Results and Discussionmentioning

confidence: 99%

“…66 For the HCPN, the N-containing zone is not easily distinguishable by looking at the N profile, because of this smoothly nitrogen-enriched interface. 3,67 The depletion of Al taking place on HCPN samples may be a beneficial biological effect, once aluminum has been assumed to be detrimental to a wide range of cells, 22 while also being commonly correlated with neurological diseases in patients who received implants, 23 but it may entail loss of mechanical properties to the alloy. The diffusion events that take place during HCPN processing form a bilayer consisting of δ-TiN, followed by ε-Ti 2 N, arranged in this order because of nitrogen interstitial filling of Ti lattices, either totally or partially.…”

Section: Chemical Profile By Glow Discharge Optical Emission Spectros...mentioning

confidence: 99%

“…Plasma surface engineering of consolidated biomaterials demands special attention to biological properties, such as cell adhesion, distribution, and proliferation. The topography of Ti6Al4V, for instance, plays a strong role in cell interactions, where rough surfaces contribute to enhanced cell adhesion, as pointed out by prior research. − Works that contemplate osseointegration report that bone growth is governed by initial stages of cell interaction with the foreign body. − Further investigating these interactions, a model has been proposed by earlier studies, in which competitive behavior between fibroblastic and osteoblastic cells is displayed, with osteoblast-like cell adhesion and proliferation being preferred for stronger bone growth and binding. , …”

Section: Introductionmentioning

confidence: 97%

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Comparative Study of Physicochemical Properties and Biocompatibility (L929 and MG63 Cells) of TiN Coatings Obtained by Plasma Nitriding and Thin Film Deposition

Fontoura

Bertele

Rodrigues

et al. 2021

ACS Biomater. Sci. Eng.

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Ti6Al4V is one of the most lightweight, mechanically resistant, and appropriate for biologically induced corrosion alloys. However, surface properties often must be tuned for fitting into biomedical applications, and therefore, surface modification is of paramount importance to carry on its use. This work compares the interaction between two different cell lines (L929 fibroblasts and osteoblast-like MG63) and medical grade Ti6Al4V after surface modification by plasma nitriding or thin film deposition. We studied the adhesion of these two cell lines, exploring which trends are consistent for cell behavior, correlating with osseointegration and in vivo conditions. Modified surfaces were analyzed through several physicochemical characterization techniques. Plasma nitriding led to a more pronounced increase in surface roughness, a thicker aluminum-free layer, made up of diverse titanium nitride phases, whereas thin film deposition resulted in a single-phase pure titanium nitride layer that leveled the ridged topography. The selective adhesion of osteoblast-like cells over fibroblasts was observed in nitrided samples but not in thin film deposited films, indicating that the competitive cellular behavior is more pronounced in plasma nitrided surfaces. The obtained coatings presented an appropriate performance for its use in biomedical-aimed applications, including the possibility of a higher success rate in osseointegration of implants.

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“…Recently, extensive research efforts have focused on the analysis of protein adsorption to synthetic surfaces (Keselowsky et al, 2003). Findings have showed that altering the surface of titanium-implant materials affects protein adsorption, cell-substrate interactions, and tissue integration (Rapuano et al, 2012;Isoshima et al, 2019;Yao et al, 2019;Zeng et al, 2019;Bayrak et al, 2020;Lin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Surface Free Energy of Titanium Disks Enhances Osteoblast Activity by Affecting the Conformation of Adsorbed Fibronectin

et al. 2022

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This study evaluated the influence of surface free energy (SFE) of titanium disks on the adsorption and conformation of fibronectin (FN) and the biological behavior of osteoblasts cultured on the FN-treated modified surfaces. High [H]-SFE titanium disks were irradiated by a 30 W UV light, while low (L)-SFE titanium disks received no treatment. The surface characteristics of the titanium disks were examined using scanning electron microscope, optical surface profilometer, X-ray photoelectron spectroscopy, and contact angle measurements. Adsorbed FN on different groups was investigated using attenuated total reflection-Fourier transform infrared spectroscopy. MG-63 cells were cultured on FN-treated titanium disks to evaluate the in vitro bioactivity. The experiment showed H-SFE titanium disks adsorbed more FN and acquired more ß-turn content than L-SFE group. MG-63 cells cultured on FN-treated H-SFE titanium disks showed better osteogenic responses, including adhesion, proliferation, alkaline phosphatase activity and mineralization than that on FN-treated L-SFE titanium disks. Compared to L-SFE titanium disks, integrin-β1, integrin-α5 and Rac-1 mRNA levels were significantly higher in MG-63 cells on FN-treated H-SFE after 3 h of culture. These findings suggest that the higher SFE of H-SFE compared to L-SFE titanium disks induced changes in the conformation of adsorbed FN that enhanced the osteogenic activity of MG-63 cells.

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Protein adsorption, cell viability and corrosion properties of Ti6Al4V alloy treated by plasma oxidation and anodic oxidation

Cited by 17 publications

References 46 publications

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Titanium and Protein Adsorption: An Overview of Mechanisms and Effects of Surface Features

Comparative Study of Physicochemical Properties and Biocompatibility (L929 and MG63 Cells) of TiN Coatings Obtained by Plasma Nitriding and Thin Film Deposition

Surface Free Energy of Titanium Disks Enhances Osteoblast Activity by Affecting the Conformation of Adsorbed Fibronectin

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