Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

Abstract

This study assesses the efficacy of anthocyanin rich Brassica olerecea leaves (ARCE) and flavonoid rich Eugenia jambolana seed (EJSE) extracts as possible cardioprotective agents against hydrogen peroxide (H(2)O(2)) induced cytotoxicity in H9C2 cells. Presence of ARCE or EJSE resulted in a superior cell viability and cell integrity as revealed by cell viability and lactate dehydrogenase release assays and acridine orange and ethidium bromide staining of control and H(2)O(2) treated H9C2 cells. These extracts were also able to reduce the impact of H(2)O(2) induced lipid peroxidation and depletion of intracellular glutathione. Also, there was an increase in mitochondrial membrane potential and reduced generation of intracellular reactive oxygen species following ARCE or EJSE treatments. These results suggest that ARCE and EJSE are capable of cardioprotective activity due to the high number of anthocyanins and flavonoids in them that are instrumental in lowering intracellular oxidative stress, preventing depletion of cellular antioxidants and improving cell viability.

Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress

“…The AO/EB staining showed that maximum number of prominent fluorescent red cells following H2O2 induced toxicity of H9C2 cells. The Brassica olerecea and Eugenia jambolana extracts were able to restore the apoptosis cell and fragmented chromatin in H9C2 cells from H2O2 treatment [35]. Similar results were obtained in the present study confirming that sodium selenite and SeNPs significantly protect the ethanol induced apoptosis in H9C2 cells.…”

Anthocyanin rich extract of Brassica oleracea L. alleviates experimentally induced myocardial infarction

“…Early studies initiated in our lab had reported ARCE mediated decrement in intracellular oxidative stress and restoration of mitochondrial membrane potential in H 2 O 2 treated H9c2 cells [ 20 ]. Also, safety evaluations had revealed that ARCE was non-toxic to H9c2 cells (10–100 μg/ml) and Swiss albino mice (1000–3000 mg/kg) [ 20 , 31 ]. Keeping these findings as a background, the present study was initiated to decipher the underlying mechanism of ARCE mediated cardioprotection.…”

“…In the present study, a dose-dependent reduction in H 2 O 2 induced cytotoxicity and apoptosis of H9c2 cells clearly implicated at ARCE mediated prevention of apoptotic cascade in oxidatively stressed cells. These results are attributable to the free radical scavenging property of ARCE [ 20 ] that had accounted for reduced cytotoxicity and prevented trigger of apoptotic cascade.…”

“…Reports on its hepatoprotective [ 16 ], membrane stabilizing [ 17 ] and neuroprotective [ 18 ] potentials have been published wherein, therapeutic potential of red cabbage has been attributed to high content of anthocyanins. Previous studies in our lab had reported that co-supplementation of Anthocyanin rich Red Cabbage Extract (ARCE) prevents cardiac and hepatic oxidative stress in atherogenic diet fed rats [ 19 ] and improves mitochondrial membrane potential in oxidatively stressed cardiomyoblasts [ 20 ]. Though, consumption of anthocyanin in reducing cardiovascular risks and myocardial infarction [ 21 ] has been reported, ARCE has not been investigated in detail for its cardioprotection.…”

Preparation of a Noncytotoxic Hemocompatible Ion Gel by Self-Polymerization of HEMA in a Green Deep Eutectic Solvent

“…The color intensity indirectly refl ects the health of cells and functional mitochondria. [ 26 ] Because of the simplicity and effectiveness of this protocol MTT assay is widely used for assessing cytotoxicity of various therapeutic agents, [27][28][29][30][31] metal complexes, [32][33][34] and nanoparticles. [ 35 ] In our study interaction of ion gel with adherent A549 cells did not account for any mortality till the highest dose (200 μg mL −1 ) (Figure 3 ).…”