Anthocyanin rich extract of Brassica oleracea L. alleviates experimentally induced myocardial infarction

Jana, Sarmita; Patel, Disha; Patel, Shweta; Upadhyay, Kapil; Thadani, Jaymesh; Mandal, Rahul Shubhra; Das, Santasabuj; Devkar, Ranjitsinh

doi:10.1371/journal.pone.0182137

Cited by 22 publications

(22 citation statements)

References 41 publications

(46 reference statements)

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“…Apoptosis markedly shares in mediation of MI including cleaved caspase‐3, TNFα, and BAX/Bcl‐2 pathway. We found the same in ISO‐ induced MI group, and this is in line with Xu et al , Kumar et al , Jana et al , and Bin‐Jaliah et al .…”

Section: Discussionsupporting

confidence: 92%

“…The B‐cell lymphoma‐2 (Bcl‐2) family is a potent regulator of the apoptotic pathways. This family of proteins includes both anti‐apoptotic and pro‐apoptotic proteins such as Bcl‐2 associated X protein (Bax) and Bcl‐2, respectively . Moreover, oxidative stress has a key role in mediating MI and antioxidants represent the first line of defense against it.…”

Section: Introductionmentioning

confidence: 99%

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Cardioprotective effect of hemin in isoprenaline‐induced myocardial infarction: role of ATP‐sensitive potassium channel and endothelial nitric oxide synthase

Refaie

Rifaai

Bayoumi

et al. 2020

Fundamemntal Clinical Pharma

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A B S T R A C TIschemic heart disease is a common cardiac health problem. Despite the significant advances in prevention and treatment of this disorder, its incidences and complications are very serious. So, the search for more antioxidants and anti-inflammatory agents with cardioprotective effects is an urgent task. We aimed to evaluate the effects of a heme oxygenase 1 (HO1) inducer, hemin (HEM), on isoprenaline (ISO)induced myocardial damage. Forty-five Wistar albino rats were used. Animals were treated with HEM (25 mg/kg/day) i.p. for 5 days and injected with ISO (150 mg/ kg/day) i.p. on 4th and 5th day of the experiment. Detection of the role of ATPsensitive potassium channel (K ATP ) was performed by administration of glibenclamide (GP) (5 mg/kg/day) orally 2 h before HEM. Moreover, the role of endothelial nitric oxide synthase (eNOS) was detected by coadministration of Nitro-x-L-arginine (L-NNA) (25 mg/kg/day) for 5 days. The ISO group showed increase in heart weight, cardiac enzymes, tumor necrosis factor alpha (TNFa), and malondialdehyde (MDA) with decrease in reduced glutathione (GSH), HO1, and total antioxidant capacity (TAC). In addition, there were increases in Bcl-2 associated X protein (Bax) and cleaved caspase-3, but decreases in B-cell lymphoma-2 (Bcl-2) and eNOS. Moreover, the histopathological examination of the ISO group showed degeneration of the cardiac muscle fibers and marked infiltration of the inflammatory cells. The biochemical and histopathological changes induced by ISO were markedly ameliorated in the HEM plus ISO group. This protective effect was diminished with coadministration of GP or L-NNA; thus, K ATP and eNOS might mediate HEM cardioprotection. I N T R O D U C T I O NCardiovascular diseases including myocardial infarction (MI) are among the most severe illnesses that could lead to death. MI occurs due to the imbalance between oxygen supply and oxygen demand followed by myocardial hypoxia [1]. This imbalance with the excessive release of reactive oxygen species (ROS) is a major contributing factor in the development of MI. Free radicals attack membrane lipids and mediate a chain of reactions of lipid peroxidation associated with disruption of the structure and permeability of cell membranes [2,3].Isoprenaline (ISO), a synthetic b-adrenergic agonist, causes severe oxidative stress and decreases oxygen supply in the myocardium leading to infarct-like necrosis in the heart muscles. The induction of MI using ISO is experimentally used to evaluate many cardiac ª 2019 Soci et e Franc ßaise de Pharmacologie et de Th erapeutique 302

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Cardioprotective effect of hemin in isoprenaline‐induced myocardial infarction: role of ATP‐sensitive potassium channel and endothelial nitric oxide synthase

Refaie

Rifaai

Bayoumi

et al. 2020

Fundamemntal Clinical Pharma

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“…Interestingly, anthocyanins from cabbage have strong antioxidant activity (22.0 mmol Fe(II)/g fw), which is approximately 10-fold higher than green cabbage (Yuan et al, 2009), suggesting for similar studies with purple cauliflower. Further, Jana et al (2017) reported total anthocyanin content in red cabbage extract was found to be 86.004 ± 3.103 mg/100 g and elucidated the mechanism of ARCE mediated prevention of experimentally induced myocardial damage in rodent model. These investigations suggests for further studies to understand the potential of purple color Brassica vegetables for good health.…”

Section: Discussionmentioning

confidence: 94%

Genetics and Expression Analysis of Anthocyanin Accumulation in Curd Portion of Sicilian Purple to Facilitate Biofortification of Indian Cauliflower

et al. 2020

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The present study was undertaken to know the genetics of purple color of cauliflower curds using a Sicilian purple 'PC-1' and a white curding mid-late group genotype of Indian cauliflower. For this, a cross was attempted between 'DC-466' (white curd) and 'PC-1' (purple curd) and observed intermediate level of purple pigmentation on curds in F 1 plants. Segregation of F 2 population (173) revealed that the purple color of the curd was governed by a single gene dominant over white, but the expression of trait was incomplete. It was substantiated by segregation of plants of BC 1 and F 2:3(intermediate) generations into 1 (white):1(intermediate) and 1(white):2(intermediate):1(intense), respectively. The F 2 , B 1 , and B 2 generations segregated into purple(intermediate to intense): white curding plants in the ratio of 126: 47, 26:24, and 40:0, respectively fitting well with the Mendelian ratio of single gene for purple curds. However, purple pigmentation on curds ranged from very light to intense, which corroborated with the wide range of anthocyanin content in F 2 (3.81-48.21 mg/100 g fw). Out of three molecular markers from high resolution map of Pr gene in purple color cauliflower 'Graffiti', only BoMYB3 marker could distinguish purple and white curding parents but did not show co-segregation while investigated in F 2 population. Expression of BoMYB1 gene was up regulated in both the purple curd genotypes 'PC-1' and 'Graffiti' in comparison to white curded 'DC-466', while BoMYB2 gene was slightly upregulated in 'PC-1' but down regulated in 'Graffiti'. Occurrence of 'broccoli type' F 2 individuals and their genetic stability in F 2:3 support the intermediate position of 'Sicilian purple' between broccoli (Calabrese) and cauliflower. There was not any correlation between curd coloration and pigmentation on apical leaf and stem portion, indicating difference of expression in 'PC-1' than 'Graffiti'. The information obtained is useful for breeding anthocyanin rich attractive purple curding 'specialty cauliflower' for better consumer health and growers' earnings.

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“…Hydrogen peroxide (H 2 O 2 ) is a major component of ROS in the cells activated by various external stimuli that trigger internal deleterious responses. In cardiomyocytes, H 2 O 2 -induced oxidative stress can stimulate MAPK signaling pathways that are closely associated with cell death and cell survival (Nam, Hah, Nam, Kim, & Park, 2016), which is why reduction of intracellular ROS production is a major target in regulating ischemic injury in cardiomyocytes (Jana et al, 2017).…”

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confidence: 99%

“…Isaak et al observed that lingonberry anthocyanins could protect cardiac cells from loss of membrane permeability and chromatin condensation (Isaak, Petkau, Blewett, Karmin, & Siow, 2017). Also, anthocyanin rich extract of Brassica oleracea L. prevented the depletion of enzymatic antioxidants (SOD and Catalase;Jana et al, 2017).…”

mentioning

confidence: 99%

Anthocyanidin attenuates myocardial ischemia induced injury via inhibition of ROS‐JNK‐Bcl‐2 pathway: New mechanism of anthocyanidin action

Syeda

Fasae

Yue

et al. 2019

Phytotherapy Research

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Despite treatment options available to date, myocardial ischemia (MI) remains the leading cause of death worldwide. Studies are focused on finding effective therapeutic strategies against MI injury. Growing interest has been developed in natural compounds possessing medicinal properties with scarcer side effects. Here, we have evaluated the cardioprotective potential of anthocyanidin against MI injury and explored its underlying protective mechanism. Left anterior descending coronary artery was ligated to induce MI in mice. Neonatal mice cardiomyocytes were treated with H2O2 to induce oxidative stress (a major contributor to MI injury) in vitro. Anthocyanidin pretreatment significantly reduced the infarct size, preserved the cell viability, and protected against ischemia‐induced cardiac injury in treatment groups compared with the H2O2‐treated group in vitro. Measurement of reactive oxygen species (ROS) validated the strong antioxidant potential of anthocyanidin, as significant reduction in oxidative stress was observed in anthocyanidin‐pretreated groups. Mechanistically, pretreatment with anthocyanidin significantly subdued the activation of JNK (to p‐JNK) and elevated Bcl‐2 levels. Both in vivo and in vitro findings suggest that anthocyanidin can induce a state of myocardial resistance against ischemic insult. We have provided the experimental evidence for inhibition of ROS/p‐JNK/Bcl‐2 pathway being the underlying mechanism of action of anthocyanidin. Our results support the use of anthocyanidin as therapeutic strategy against MI injury.

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Anthocyanin rich extract of Brassica oleracea L. alleviates experimentally induced myocardial infarction

Cited by 22 publications

References 41 publications

Cardioprotective effect of hemin in isoprenaline‐induced myocardial infarction: role of ATP‐sensitive potassium channel and endothelial nitric oxide synthase

Cardioprotective effect of hemin in isoprenaline‐induced myocardial infarction: role of ATP‐sensitive potassium channel and endothelial nitric oxide synthase

Genetics and Expression Analysis of Anthocyanin Accumulation in Curd Portion of Sicilian Purple to Facilitate Biofortification of Indian Cauliflower

Anthocyanidin attenuates myocardial ischemia induced injury via inhibition of ROS‐JNK‐Bcl‐2 pathway: New mechanism of anthocyanidin action

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