Prospects for application of breakthrough technologies in breeding: The CRISPR/Cas9 system for plant genome editing

Хлесткина, Е. К.; Shumny, V. K.

doi:10.1134/s102279541607005x

Cited by 21 publications

(13 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plant genome editing uses sequence-specific nucleases ( SSNs ) for stably inherited and predetermined gene modification by introducing favorable alleles into our crop of interest that results in a transgene-free desired genome ( 66 ). SSNs cause certain changes at the chromosomal level, which results in deletion, insertion, or substitution of specific nucleotide sequence at particular loci.…”

Section: Breeding Strategies To Develop Pst -Resismentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

View full text Add to dashboard Cite

Puccinia striiformis (Pst) is a devastating biotrophic fungal pathogen that causes wheat stripe rust. It usually loves cool and moist places and can cause 100% crop yield losses in a single field when ideal conditions for disease incidence prevails. Billions of dollars are lost due to fungicide application to reduce stripe rust damage worldwide. Pst is a macrocyclic, heteroecious fungus that requires primary (wheat or grasses) as well as secondary host ( Berberis or Mahonia spp.) for completion of life cycle. In this review, we have summarized the knowledge about pathogen life cycle, genes responsible for stripe rust resistance, and susceptibility in wheat. In the end, we discussed the importance of conventional and modern breeding tools for the development of Pst -resistant wheat varieties. According to our findings, genetic engineering and genome editing are less explored tools for the development of Pst -resistant wheat varieties; hence, we highlighted the putative use of advanced genome-modifying tools, i.e., base editing and prime editing, for the development of Pst -resistant wheat.

show abstract

Section: Breeding Strategies To Develop Pst -Resismentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The application of molecular markers was successful in the study of wheat genes controlling such traits as 1,000-grain weight, protein and gluten content (Zhang et al, 2018), grain hardness (Nirmal et al, 2016), flour production from grain milling (Nirmal et al, 2017), and bread quality (Henry, Furtado & Rangan, 2018). Genome editing using CRISPR/Cas9 technology represents a novel method in plants (Khlestkina & Shumny, 2016; Liang et al, 2018; Borisjuk et al, 2019), for production of wheat with low gluten content (Sánchez-León et al, 2018), as required by people allergic to some components of gliadin in traditional wheat cultivars (Palosuo et al, 2001; Pastorello et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Utebayev

Dashkevich

Bome

et al. 2019

PeerJ

View full text Add to dashboard Cite

Background Spring bread wheat (Triticum aestivum L.) represents the main cereal crop in Northern Kazakhstan. The quality of wheat grain and flour strongly depends on the structure of gluten, comprised of gliadin and glutenin proteins. Electrophoresis spectra of gliadins are not altered by environmental conditions or plant growth, are easily reproducible and very useful for wheat germplasm identification in addition to DNA markers. Genetic polymorphism of two Gli loci encoding gliadins can be used for selection of preferable genotypes of wheat with high grain quality. Methods Polyacrylamide gel electrophoresis was used to analyse genetic diversity of gliadins in a germplasm collection of spring bread wheat from Northern Kazakhstan. Results The highest frequencies of gliadin alleles were found as follows, in Gli1: -A1f (39.3%), -B1e (71.9%), and -D1a (41.0%); and in Gli-2: -A2q (17.8%), -B2t (13.5%), and -D2q (20.4%). The combination of these alleles in a single genotype may be associated with higher quality of grain as well as better adaptation to the dry environment of Northern Kazakhstan; preferable for wheat breeding in locations with similar conditions.

show abstract

“…The authors compare various techniques of optimizing the CRISPR/Cas9 system that can expand its capabilities: in silico selection of target sites, vector design, vector transfer techniques, transfer efficiency and expression estimation, etc. (Khlestkina and Shumnyi, 2016;Gerasimova et al, 2017). Another survey of this team analyzes the use of the CRISPR/Cas9 modification of agricultural plants.…”

Section: Gene Engineering As a Methods Of Modifying Plant Resourcesmentioning

confidence: 99%

Gene Engineering of Lamiaceae Family: Basic and Applied Research

Cherednichenko¹,

Polivanova²,

Khlebnikova³

et al. 2021

revistageintec

View full text Add to dashboard Cite

Gene engineering is a convenient way of solving basic and applied tasks related to the system of secondary metabolism of medicinal and aromatic plants, such as a large and economically important plant family Lamiaceae Martinov (Labiatae Juss.) that includes such major species, as Agastache, Dracocephalum, Lavandula, Mentha, Ocimum, Salvia, Satureja, Thymus, etc. The basic value of this method consists in studying the genetic mechanism of secondary metabolism, which includes key enzyme genes, transcription factors, etc. Applied research consists in producing valuable secondary metabolites with biological activity, including active pharmaceutical ingredients in both, the familyʹs plants proper and the heterologous systems using relevant plant genes. This overview considers a set of diverse gene engineering studies.

show abstract

Prospects for application of breakthrough technologies in breeding: The CRISPR/Cas9 system for plant genome editing

Cited by 21 publications

References 97 publications

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Gene Engineering of Lamiaceae Family: Basic and Applied Research

Contact Info

Product

Resources

About