Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isoenzyme loci of 47 reference varieties

Abstract

Fifty-nine spring and 7 winter barley varieties in 'The Danish List of Varieties of Agricultural Crops, 1983/84' were examined for variation at 39 isoenzyme and two hordein loci. Twenty-three isoenzyme loci had one allele only, and 16 loci had from two to five alleles. One hordein locus had 12 and the other 15 alleles. The variation in the 16 enzyme loci permitted the division of the 66 varieties into 63 groups, while the two hordein loci produced 34 groups. A study of 20 individuals from each variety showed that 22 varieties were polymorphic in at least one locus. Eight starch gel electrophoresis with various buffer systems, one agar gel electrophoresis (for amylases), and one polyacrylamide gel electrophoresis (for hordein) were performed to develop the patterns associated with the 4 1 loci. The polyacrylamide gel electrophoresis developing hordein patterns was clearly the most powerful single system for identifying barley varieties because of the large number of alleles.

Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

“…X canadensis (Rajora, 1986 ;Rajora & Zsuffa, 1989), and in Cryptomeria japonica (Miyazaki & Miyajima, 1981) . Isozyme markers have been found to be appropriate for identification of cultivars, and commercial and botanical varieties of several agricultural and related plants (e .g ., Fedak, 1974 ;Bassiri & Rouhani, 1977 ;Bassiri & Adams, 1978 ;Cardy & Kannenberg, 1982 ;Ignart & Weeden, 1984 ;Weeden, 1984 ;Nielsen, 1985 ;Nielsen & Johansen, 1986 ; among others) .…”

“…Alternately, the application of biochemical markers, especially those under Mendelian genetic control, such as allozymes offers powerful tools for such studies (Adams, 1983 ;Bailey, 1983 ;Nielsen, 1985) . In recent years, isozyme polymorphism has been widely applied by plant breeders for identification and classification of cultivars and varieties of agricultural and related plants (e .g ., Fedak, 1974 ;Bassiri & Rouhani, 1977 ;Werner & Sink, 1977 ;Bassiri & Adams, 1978 ;Cardy & Kannenberg, 1982 ;Ignart & Weeden, 1984 ;Weeden, 1984 ;Nielsen, 1985 ;Nielsen & Johansen, 1986 ;Glaszmann, 1987 ;Ramirez et al ., 1987) . In forest trees, the application of isozyme analysis for identification of clones, cultivars and varieties has been limited (Miyazaki & Miyajima, 1981 ;Adams, 1983 ;Cheliak & Pitel, 1984 ;Rajora, 1986) .…”

Variation among pollen specific isozymes in barley

“…7). This isozyme is designated NDH-5 and migrates cathodally relative to the four previously described NDH isozymes (BROWN 1983;NIELSEN and JOHANSEN 1986).…”

Genetic profiles and diversity of Baltic spring barley material

“…Eight isozyme loci where characterised. The designation of alleles at the Aco-1 and Pgd-2 loci followed that of Nielsen and Johansen (1986), at Est-1; Est-2, Est-4 and Est-5 loci Hvid followed Nielsen (1977) and at Aco-2 locus - Liu et al (1999). …”