Properties of hydrodynamic J-type countercurrent chromatography for protein separation using aqueous two-phase systems: With special reference to constructing conical columns

Ding, Jianjian; Li, Shifei; Zhao, Yuqing; Guan, Yue; Deng, Li Gang; Deng, Qiuyun

doi:10.1016/j.chroma.2017.03.076

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…For this purpose, the top phase of the prepared ATPS can be used as the solvent to extract the solute or as a reaction medium for the investigation of specific reactions. − The upper phase of PEG-ammonium sulfate ATPSs has been used as a mobile phase in studies using countercurrent chromatography to separate nucleosides . Other studies have used the upper or lower phases of the ATPSs in countercurrent chromatography to investigate the separation of myoglobin and lysozyme and the retention of the stationary phase, as well as understand the hydrodynamic phase distribution and retention . In another study, the upper and lower phases were used for sample solution preparation, respectively .…”

Section: Introductionmentioning

confidence: 99%

“…Polymer salts are commonly used in two types of ATPSs. − In particular, polymer-based sulfate salt has been frequently studied as sulfate salt can easily split phases and has a relatively larger binodal area in the formed ATPS. − The PEG-sulfate ammonium ATPS is often used to separate hydrophilic substances, such as phenols, nucleosides, etc. − In this study, emphasis was placed on the dissolubility of the top phase of the ATPS. Therefore, an easy splitting process with a relatively highly hydrophilic ATPS is required.…”

Section: Introductionmentioning

confidence: 99%

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Solubility of Naphthalene in the Top Phase of Polyethylene Glycol and Ammonium Sulfate Aqueous Two-Phase Systems

Liu

Zhu

et al. 2023

J. Chem. Eng. Data

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In this study, the solubility of naphthalene was used to demonstrate the dissolubility of three top aqueous two-phase systems (ATPSs) comprising polyethylene glycol (PEG) 1000, PEG 1500, and PEG 2000, respectively, mixed with ammonium sulfate (NH 4 ) 2 SO 4 . First, the liquid−liquid equilibrium data were obtained for the three individual ATPSs. Under the condition of 1 mg naphthalene in a 1 mL individual top phase of the ATPSs at 298 K, the measured dissolubilities with compositions of 20 wt % ammonium sulfate and 20 wt % PEG 1000, 1500, and 2000 against naphthalene were 4.19, 4.80, and 5.04 mmol•L −1 , respectively. It was demonstrated that the dissolubility of naphthalene in the top phase of ATPS positively correlates with the contents of PEG, salt, and both (PEG and salt), with average correlation coefficients of 0.9817, 0.9978, and 0.9588, respectively. The dissolubilities of the top phases of the three ATPSs against naphthalene were compared and revealed to be within 98−163% of those of 13 selected common solvents, indicating that their performance can be further fine-tuned. The results obtained in this study highlight characteristics of the ATPSs that render them suitable for use as solvents, in addition to their well-known use as separation candidates.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Solubility of Naphthalene in the Top Phase of Polyethylene Glycol and Ammonium Sulfate Aqueous Two-Phase Systems

Liu

Zhu

et al. 2023

J. Chem. Eng. Data

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“…Article Related Abbreviations: RA, Rebaudioside A; S f , the retention rate of the stationary phase; ST, Stevioside In past years, the researchers have made a lot of efforts in different ways including: instrument improvement [4][5][6], coupling technique [7,8], new elution mode [9][10][11], solventmodified [12,13], new separation strategy, and the inner structure of CCC column [14][15][16] to overcome the deficiency of CCC. Recently, a graphene oxide modified novel CCC column that combined the separation mode of partition and adsorption chromatography has been prepared by our group to improve the CCC capability on separating the compounds with the similar structures [17].…”

Section: Introductionmentioning

confidence: 99%

Counter‐current chromatography melamine‐modified column and its separation mechanism

Quan

Huang

Gong

et al. 2018

J of Separation Science

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In this work, to further verify and develop the novel counter‐current chromatography modified column separate mode, a melamine modified counter‐current chromatography column was prepared. Meanwhile, the modified counter‐current chromatography column was used to separate stevioside and rebaudioside A with the same partition coefficient in chosen solvent system to evaluate its separation efficiency. The results show that because of the presence of intermolecular forces between melamine and model compounds, better separation could be achieved on the modified column while it's almost impossible to be separated on the unmodified column. So the results of this research further show that column modified method is a possible approach to further increase the separation ability of counter‐current chromatography. Take advantage of large sample handing capacity of counter‐current chromatography, the mothed may have great potential to be an efficient method of separation and preparation enantiomer compounds.

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Ionic Liquids as Modifying Agents for Protein Separation in Centrifugal Partition Chromatography

2019

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Aqueous two‐phase systems (ATPSs) can be applied in centrifugal partition chromatography (CPC) to separate biomolecules. Two ionic liquids (ILs) served as modifying agents to tune partition coefficients in the separation of two model proteins, myoglobin and lysozyme. Myoglobin was not affected by the ILs, while the partition coefficient of lysozyme was lowered with addition of IL. High stationary phase retention was achieved with ATPSs in a CPC column with cell size and geometry specially designed for unstable biphasic systems. When ILs were used as modifying agents, no notable decrease in stationary phase retention was observed. The injection volume was increased to 20 % of the total column volume and process simulations indicate that a further increase in the injection volume is possible.

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Properties of hydrodynamic J-type countercurrent chromatography for protein separation using aqueous two-phase systems: With special reference to constructing conical columns

Cited by 6 publications

References 44 publications

Solubility of Naphthalene in the Top Phase of Polyethylene Glycol and Ammonium Sulfate Aqueous Two-Phase Systems

Solubility of Naphthalene in the Top Phase of Polyethylene Glycol and Ammonium Sulfate Aqueous Two-Phase Systems

Counter‐current chromatography melamine‐modified column and its separation mechanism

Ionic Liquids as Modifying Agents for Protein Separation in Centrifugal Partition Chromatography

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