Ionic Liquids as Modifying Agents for Protein Separation in Centrifugal Partition Chromatography

Abstract

Aqueous two‐phase systems (ATPSs) can be applied in centrifugal partition chromatography (CPC) to separate biomolecules. Two ionic liquids (ILs) served as modifying agents to tune partition coefficients in the separation of two model proteins, myoglobin and lysozyme. Myoglobin was not affected by the ILs, while the partition coefficient of lysozyme was lowered with addition of IL. High stationary phase retention was achieved with ATPSs in a CPC column with cell size and geometry specially designed for unstable biphasic systems. When ILs were used as modifying agents, no notable decrease in stationary phase retention was observed. The injection volume was increased to 20 % of the total column volume and process simulations indicate that a further increase in the injection volume is possible.

Counteracting Bleeding in Centrifugal Partition Chromatography: Redosing of the Stationary Phase

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Continuous Industrial-Scale Centrifugal Partition Chromatography with Automatic Solvent System Handling: Concept and Instrumentation

“…Even with today’s solvent restrictions, there are plenty of preferred solvents − to choose from and try out. Also, emerging new solvents, such as fluorinated solvents, deep eutectic solvents, , or ionic liquids, − are opening up new possibilities for the solvent system selection.…”

Generalizing property prediction of ionic liquids from limited labeled data: a one-stop framework empowered by transfer learning

“…In recent years, ILs have attracted remarkable attention in various applications, both in chemistry and engineering, 1,2 due to their unique physicochemical properties such as negligible vapor pressure, high thermal and electrochemical stability, wide liquidus range, etc. 2,3 More importantly, ILs also offer great potential to tune their physical and chemical properties by judicious selection of the cations and anions. For this reason, ILs could be designed to offer desirable properties to meet specific requirements for arbitrary given applications.…”