Prooxidant-antioxidant state of the organism during oxidative stress and correction of the L-arginine-NO system

Glebov, Anastasia; Зинчук, В. В.

doi:10.1007/s10517-006-0178-0

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Oxidative damage plays a key role in inflammatory reactions and can induce several injuries (e.g., septic shock, atherosclerose) induced by PMA and/or LPS which are known to enhance the formation of reactive oxygen species (ROS) (Glebov and Zinchuk 2006;Swindle et al 2002). PMA/LPS can induce the activation of TLR4 in the cell wall, which can induce ROS, e.g., superoxide (Swindle et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Actovegin® reduces PMA-induced inflammation on human cells

et al. 2020

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Purpose The effect of Actovegin® was investigated on PMA- and LPS-induced human peripheral blood mononuclear cells (PBMCs). Methods PBMCs (1 × 106 cells/ml) from five blood donors (2 f, 3 m; 45–55 years) were grown in medium and exposed to Actovegin® in the presence or absence of PMA or LPS. Supernatants were collected to assess the concentration of cytokines (TNF-α, IL-1beta, IL-6 and IL-10). The reactive oxygen species (ROS) were assessed by a ROS-GloTM H2O2 assay. Results Stimulation of cells by PMA or LPS (without Actovegin®) significantly increased the secretion of IL-1beta, IL-6, IL-10 and TNF-α from PBMCs, compared to controls. Pre-treatment of cells with Actovegin® (1, 5, 25, 125 µg/ml) plus PMA significantly decreased the secretion of IL-1beta from PBMCs, compared to controls (PMA without Actovegin®). In contrast, addition of Actovegin® (1, 5, 25, 125 and 250 µg/ml) plus LPS did not alter the IL-1beta production, compared to controls (LPS without Actovegin®). TNF-α, IL-6 and IL-10 do not contribute to the reduction of inflammatory reactions with Actovegin®. Conclusions Actovegin® can reduce the PMA-induced IL-1beta release and the ROS production from PBMCs. These findings may help to explain the clinically known positive effects of Actovegin® on athletic injuries with inflammatory responses (e.g., muscle injuries, tendinopathies).

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Section: Discussionmentioning

confidence: 99%

Actovegin® reduces PMA-induced inflammation on human cells

et al. 2020

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“…However, because the stress mechanism is insufficiently understood, the application of this therapy is intuitive; in one set of cases it induces sensitization. 2,3 The remaining basic contradiction concerns the interpretation of the role of constancy and variability of the genome in adaptation. In consequence, the research of the mechanism of gene stress under conditions of exposure to various kinds of stressors is important Address for correspondence: S. S. Sherlina According to recent concepts, a reaction at the level of the genome and participation of genetic elements in intercellular signaling (horizontal transfer of genes) are some of the early manifestations of exposure to stress.…”

Section: Introductionmentioning

confidence: 99%

“…At present, positive data on the correction of this state were obtained by means of antioxidative therapy. However, because the stress mechanism is insufficiently understood, the application of this therapy is intuitive; in one set of cases it induces sensitization 2,3 . The remaining basic contradiction concerns the interpretation of the role of constancy and variability of the genome in adaptation.…”

Section: Introductionmentioning

confidence: 99%

The Involvement of Different Mobile LINE Copies of Blood Plasma and Extrachromosomal DNA of Liver Cells in Systemic Adaptive Response

Sherlina¹,

Vladimirov²

2008

Annals of the New York Academy of Sciences

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The aim of this work was to investigate the dynamics of the quantity of LINE in the DNA composition of blood plasma and extrachromosomal DNA of liver cells under conditions of either immobilization or exposure to ionizing radiation or a combination of both factors. It has been established that post-stress changes in the quantity of genetic elements may be interpreted as an activation of the mechanisms of retroposition and intercellular transfer of genetic elements that ensure the formation of adaptive responses at the systemic level. The formation of adaptive responses is expressed through the reduction of the content of the low-molecular-weight fraction of the extracellular DNA.

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Anti-inflammatuar and anti-oxidative effects of Nigella sativa L.: 18FDG-PET imaging of inflammation

et al. 2014

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Inflammation has an important role in many diseases such as cystic fibrosis, allergies and cancer. The free radicals produced during inflammation, can induce gene mutations and posttranslational modifications of cancer related proteins. Nigella sativa L. (N. sativa) is herbaceous plant and commonly used as a natural food. It has many pharmacological effects including antibacterial, antifungal, antitumor, analgesic, antipyretic activity. The aim of this study was to investigate the anti-inflammatuar and anti-oxidant activity of N. sativa in acute inflammation. Thus we used the experimental lipopolysaccharides (LPS)-induced model. Intraperitoneal LPS 1 mg/kg was administered to groups. N. sativa (500 mg/kg) and essential oil (5 ml/kg) were given orally to treatment groups, after 24-h of intraperitoneal LPS-injection. To determine the lung inflammation, 18F-fluoro-deoxy-D-glucose (0.8 ml/kg) was administrated under the anesthesia before the 1 h of PET-scanning. After the FDG-PET, samples were collected. Lung and liver 18F-FDG-uptake was calculated. Serum AST, ALT, LDH and hcCRP levels were determined and liver, lung and erythrocyte SOD, MDA and CAT levels were measured. Liver and lung NO and DNA fragmentation levels were determined. MDA levels were decreased in treated inflammation groups whereas increased in untreated inflammation group. SOD and CAT activities in untreated inflammation group were significantly lower. According to the control group, increased AST and ALT levels were found in untreated inflammation group. 18F-FDG uptake of inflammation groups were increased when compare the control group. We found increased 18F-FDG uptake, DNA fragmentation and NO levels in LPS-induced inflammation groups. We conclude that, in LPS-induced inflammation, N. sativa have therapeutic and anti-oxidant effects.

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Prooxidant-antioxidant state of the organism during oxidative stress and correction of the L-arginine-NO system

Cited by 3 publications

References 5 publications

Actovegin® reduces PMA-induced inflammation on human cells

Actovegin® reduces PMA-induced inflammation on human cells

The Involvement of Different Mobile LINE Copies of Blood Plasma and Extrachromosomal DNA of Liver Cells in Systemic Adaptive Response

Anti-inflammatuar and anti-oxidative effects of Nigella sativa L.: 18FDG-PET imaging of inflammation

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