Anti-inflammatuar and anti-oxidative effects of Nigella sativa L.: 18FDG-PET imaging of inflammation

Abstract

Inflammation has an important role in many diseases such as cystic fibrosis, allergies and cancer. The free radicals produced during inflammation, can induce gene mutations and posttranslational modifications of cancer related proteins. Nigella sativa L. (N. sativa) is herbaceous plant and commonly used as a natural food. It has many pharmacological effects including antibacterial, antifungal, antitumor, analgesic, antipyretic activity. The aim of this study was to investigate the anti-inflammatuar and anti-oxidant activity of N. sativa in acute inflammation. Thus we used the experimental lipopolysaccharides (LPS)-induced model. Intraperitoneal LPS 1 mg/kg was administered to groups. N. sativa (500 mg/kg) and essential oil (5 ml/kg) were given orally to treatment groups, after 24-h of intraperitoneal LPS-injection. To determine the lung inflammation, 18F-fluoro-deoxy-D-glucose (0.8 ml/kg) was administrated under the anesthesia before the 1 h of PET-scanning. After the FDG-PET, samples were collected. Lung and liver 18F-FDG-uptake was calculated. Serum AST, ALT, LDH and hcCRP levels were determined and liver, lung and erythrocyte SOD, MDA and CAT levels were measured. Liver and lung NO and DNA fragmentation levels were determined. MDA levels were decreased in treated inflammation groups whereas increased in untreated inflammation group. SOD and CAT activities in untreated inflammation group were significantly lower. According to the control group, increased AST and ALT levels were found in untreated inflammation group. 18F-FDG uptake of inflammation groups were increased when compare the control group. We found increased 18F-FDG uptake, DNA fragmentation and NO levels in LPS-induced inflammation groups. We conclude that, in LPS-induced inflammation, N. sativa have therapeutic and anti-oxidant effects.

BLACK CUMIN (Nigella sativa) AND ITS ACTIVE COMPONENT OF THYMOQUINONE: EFFECTS ON HEALTH

“…Nigella sativa is used in the treatment of many diseases in many countries globally. Its beneficial effects on health, especially against diseases such as cancer, diabetes and cardiovascular disease have been highlighted (Bamosa, 2015;Entok et al, 2014;Leong et al, 2013;Shafiq et al, 2014;Singh et al, 2014). The effects of N. sativa on health are shown in Figure 1.…”

“…It has also been highlighted that the active substances of N. sativa have antibacterial, antifungal, antidiabetic, immunomodulator, anti-inflammatory, analgesic, antiviral, antioxidant, anticonvulsant, antihypertensive, anticancer and antihyperlipidemic effects (Entok et al, 2014;Leong, Rais Mustafa, & Jaarin, 2013;Shafiq, Ahmad, Masud, & Kaleem, 2014;Singh et al, 2014). Due to these effects, N. sativa seed and oil have been used globally in the treatment of many diseases such as asthma, diarrhoea, dysentery, dyspepsia, fever, icterus, apoplexy, hemorrhoids and cardiovascular, digestive, immune-system, liver, respiratory and kidney diseases (Forouzanfar, Bazzaz, & Hosseinzadeh, 2014).…”

Flexural and Surface Properties of PMMA Denture Base Material Modified with Thymoquinone as an Antifungal Agent

“…9 Thymoquinone (TQ) is extracted from Nigella sativa seeds, also called black seeds or black cumin; they grow in South and Southwest Asia. 10,11 The extracts of Nigella sativa (essential oil, fixed oil, proteins, alkaloids, and saponins) have shown anti-inflammatory, antibacterial, antioxidant, and antitumor effects. [12][13][14] Their effect against dental caries, oral ulcers, and periodontal diseases has been tested.…”

Recycling and Standardization Aspects of Nigella sativa in the Food Industr

“…The growth of scientific interest in black cumin and the popularity of black cumin oil among the population noted in recent years should probably be attributed to the popularization of scientific data on the pronounced physiological properties of both the oil itself and the seeds as a whole. The number of publications annually confirms the pronounced therapeutic effect of seeds and black cumin oil on a number of diseases associated with the respiratory, gastrointestinal, immune, cardiovascular and nervous systems, including type 2 diabetes and cancer [10,[12][13][14][15][16].…”