Proof-of-concept demonstration of a total internal reflection based module for fluorescence and absorbance detection using a 3D-printed syringe pump

Verschooten, Tom; Ottevaere, Heidi; Vervaeke, Michael; Erps, Jürgen Van; Thienpont, Hugo

doi:10.1117/12.2052082

Cited by 2 publications

(12 citation statements)

References 9 publications

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“…The first experiment involves setting up a calibration curve for the detection of Coumarin 480 dissolved in ethanol. This allows direct comparison of our current system's LIF/ABS performance to that of our previous work [11,12]. The second experiment investigates the system's sensitivity to turbidity.…”

Section: Calibrationmentioning

confidence: 98%

“…Misalignment of the chip should hence not deviate the excitation beam. (iii) The system presented in our previous work [11,12] was highly sensitive to presence of air bubbles. In case one or more air bubbles were present, the measurement had to be repeated.…”

Section: Optofluidic Chip Designmentioning

confidence: 98%

“…As we are aiming to outperform our previous work [11,12] we should select a chip design in which the interaction length exceeds 3 mm to achieve higher sensitivity for ABS and LIF. On the other hand, a chip with a longer interaction length will also exhibit a larger internal volume.…”

Section: Optofluidic Chip Designmentioning

confidence: 99%

“…We use two excitation laser diodes with respectively 405 nm and 450 nm wavelengths as these show good compatibility with coumarin 480 [11,12] and lubricant oils [1,10]. We show the properties of these laser diodes in table 1.…”

Section: Demonstrator Setupmentioning

confidence: 99%

“…Using this system we set up a LIF-ABS calibration curve for coumarin 480, allowing direct comparison with our previous multi-measurement system [11,12]. We also set up a turbidity calibration curve using microsphere standards to demonstrate the system's ability to detect various amounts of scattered light and the correlation to sample turbidity.…”

Section: Introductionmentioning

confidence: 99%

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Optofluidic multi-measurement system for the online monitoring of lubricant oil

Verschooten

Callewaert

Ciaccheri

et al. 2015

Meas. Sci. Technol.

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We show a detection system that simultaneously allows absorbance (ABS), laser-induced fluorescence (LIF) and scattering detection excited by two different laser sources at 405 nm and 450 nm. The heart of the system consists of a mass manufacturable polymer optofluidic chip. The chip is mounted in an optical detection assembly that aligns the chip to the rest of the system, seals the chip from leakage, fixes the position and connects the channels to the rest of the fluidic system. The fluidics exhibit a reduced susceptibility to perturbations caused by air bubbles, this is accomplished by making use of a serpentine channel layout. For coumarin 480, detection limits of 100 nM and 10 pM are observed for ABS and LIF respectively. An effective detection range of 4000 down to 1 nephelometric turbidity units is shown for the detection of scattered light. The viscous behaviour of the sample is analysed by a secondary FFT processing step of which the result is further processed by multivariate data analysis. This allows the identification of samples and prediction of their quality parameters. We apply this system for the monitoring of lubricant oil, demonstrating its ability to compete with spectroscopic detection techniques. The low-cost approach and multi-measurement architecture shown in this paper pave the way for miniaturized on-line monitoring of liquids in an industrial environment.

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Section: Calibrationmentioning

confidence: 98%

Section: Optofluidic Chip Designmentioning

confidence: 98%

Section: Optofluidic Chip Designmentioning

confidence: 99%

Section: Demonstrator Setupmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Verschooten

Callewaert

Ciaccheri

et al. 2015

Meas. Sci. Technol.

Self Cite

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Indirect Rapid Prototyping: Opening Up Unprecedented Opportunities in Scaffold Design and Applications

et al. 2016

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Over the past decades, solid freeform fabrication (SFF) has emerged as the main technology for the production of scaffolds for tissue engineering applications as a result of the architectural versatility. However, certain limitations have also arisen, primarily associated with the available, rather limited range of materials suitable for processing. To overcome these limitations, several research groups have been exploring novel methodologies through which a construct, generated via SFF, is applied as a sacrificial mould for production of the final construct. The technique combines the benefits of SFF techniques in terms of controlled, patient-specific design with a large freedom in material selection associated with conventional scaffold production techniques. Consequently, well-defined 3D scaffolds can be generated in a straightforward manner from previously difficult to print and even "unprintable" materials due to thermomechanical properties that do not match the often strict temperature and pressure requirements for direct rapid prototyping. These include several biomaterials, thermally degradable materials, ceramics and composites. Since it can be combined with conventional pore forming techniques, indirect rapid prototyping (iRP) enables the creation of a hierarchical porosity in the final scaffold with micropores inside the struts. Consequently, scaffolds and implants for applications in both soft and hard tissue regeneration have been reported. In this review, an overview of different iRP strategies and materials are presented from the first reports of the approach at the turn of the century until now.

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Proof-of-concept demonstration of a total internal reflection based module for fluorescence and absorbance detection using a 3D-printed syringe pump

Cited by 2 publications

References 9 publications

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Indirect Rapid Prototyping: Opening Up Unprecedented Opportunities in Scaffold Design and Applications

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